FastQCFastQC Report
Thu 9 Feb 2017
SRR2727394.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727394.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences954755
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT56760.5944980649485994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24240.2538871228744547No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21920.22958769527260922No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15190.15909840744484188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGAC806.95758E-720.81253
TATACTG1850.018.05
TAGACTG1705.456968E-1217.4117645
CTTATAC6400.017.3437537
GGGTAAG2250.017.2666651
GCGACGT650.001579668617.07692533
GGCGAAA650.001579668617.07692510
CAGGACT3250.017.0769234
GGTATCA13550.016.2472321
GTTACGG700.002592062815.85714225
GTACTAA700.002592062815.8571421
GCCTAAA1059.339703E-615.8571421
TAGAGTG1659.731593E-1015.696975
GCGAAAG957.057675E-515.57894811
ACCCGAC2150.015.48837211
GTATTGG2055.456968E-1215.3414631
ATTAGAC855.36297E-415.2352943
GAATCCG855.36297E-415.2352945
CTAGTAC855.36297E-415.2352943
CCTACAC2201.8189894E-1215.1363643
Produced by FastQC (version 0.11.5)