##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727394.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	954755
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39444045854696	33.0	31.0	34.0	31.0	34.0
2	32.54294557242434	34.0	31.0	34.0	31.0	34.0
3	32.63446748118627	34.0	31.0	34.0	31.0	34.0
4	36.154267848819856	37.0	35.0	37.0	35.0	37.0
5	35.96624369602673	37.0	35.0	37.0	35.0	37.0
6	35.97813260993658	37.0	35.0	37.0	35.0	37.0
7	35.941470848542295	37.0	35.0	37.0	35.0	37.0
8	35.90051793392022	37.0	35.0	37.0	35.0	37.0
9	37.739348838183616	39.0	37.0	39.0	35.0	39.0
10	37.57138742399883	39.0	37.0	39.0	35.0	39.0
11	37.68043424752947	39.0	37.0	39.0	35.0	39.0
12	37.60157108368115	39.0	37.0	39.0	35.0	39.0
13	37.672308602730546	39.0	37.0	39.0	35.0	39.0
14	38.94261878701866	40.0	38.0	41.0	36.0	41.0
15	38.871832040680594	40.0	38.0	41.0	36.0	41.0
16	38.84692303261046	40.0	38.0	41.0	35.0	41.0
17	38.780211677341306	40.0	38.0	41.0	35.0	41.0
18	38.747391739242005	40.0	38.0	41.0	35.0	41.0
19	38.79131819157794	40.0	38.0	41.0	35.0	41.0
20	38.754451141915986	40.0	38.0	41.0	35.0	41.0
21	38.72046964928175	40.0	38.0	41.0	35.0	41.0
22	38.67918575969751	40.0	38.0	41.0	34.0	41.0
23	38.61772077653429	40.0	38.0	41.0	34.0	41.0
24	38.5786782996685	40.0	38.0	41.0	34.0	41.0
25	38.47401898916476	40.0	38.0	41.0	34.0	41.0
26	38.37683489481595	40.0	38.0	41.0	34.0	41.0
27	38.29236296222591	40.0	38.0	41.0	34.0	41.0
28	38.20966844897382	40.0	38.0	41.0	34.0	41.0
29	38.14143837947955	40.0	38.0	41.0	34.0	41.0
30	38.087345968337424	40.0	38.0	41.0	33.0	41.0
31	38.00388057669245	40.0	37.0	41.0	33.0	41.0
32	37.91988834831972	40.0	37.0	41.0	33.0	41.0
33	37.83293829306995	40.0	37.0	41.0	33.0	41.0
34	37.748561672889906	40.0	37.0	41.0	33.0	41.0
35	37.699460070908245	40.0	37.0	41.0	33.0	41.0
36	37.61928871804808	40.0	37.0	41.0	33.0	41.0
37	37.546329686673545	40.0	37.0	41.0	33.0	41.0
38	37.46320050693633	40.0	37.0	41.0	32.0	41.0
39	37.36751836858671	40.0	37.0	41.0	32.0	41.0
40	37.268265680724376	40.0	37.0	41.0	31.0	41.0
41	37.17488465627307	39.0	36.0	41.0	31.0	41.0
42	37.07417609753288	39.0	36.0	41.0	31.0	41.0
43	35.428071075825734	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	0.0
14	1.0
15	4.0
16	5.0
17	9.0
18	14.0
19	39.0
20	84.0
21	168.0
22	329.0
23	598.0
24	1054.0
25	1773.0
26	2714.0
27	4181.0
28	6183.0
29	8732.0
30	11711.0
31	15877.0
32	20558.0
33	27235.0
34	36998.0
35	51734.0
36	76785.0
37	130565.0
38	261386.0
39	296014.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.84580337364036	18.141303266282975	12.056286691350136	25.956606668726533
2	16.521725468837555	20.424716288471913	37.54366303397207	25.50989520871847
3	17.926902713261516	22.646961786007928	31.09488821739609	28.331247283334466
4	12.791344376305963	15.157710616859823	36.65882870474624	35.39211630208797
5	13.62632298338317	37.660761137674065	35.56703028525643	13.145885593686337
6	34.654335405418145	37.28401527093338	14.961325156715596	13.100324166932879
7	29.5491513529649	31.32143848421847	21.428115066168807	17.701295096647833
8	27.24133416426204	36.91439165021393	18.99429696623741	16.849977219286625
9	26.629973134469054	13.340176275589025	18.717890977266418	41.3119596126755
10	14.345669831527461	27.982780922854555	35.43359291127043	22.237956334347555
11	37.047095851815385	21.046760687296743	22.330545532623553	19.57559792826432
12	20.658598279139675	25.190965221444245	32.25141528454944	21.89902121486664
13	31.196275484286545	19.427078150939245	25.057004152897868	24.319642211876342
14	21.326832538190427	19.398379689030172	27.63431456237464	31.640473210404764
15	24.483034914716338	28.624725714973998	22.647380741656235	24.244858628653425
16	24.15185047472912	26.715440086723817	24.806049719561564	24.3266597189855
17	23.564265177977596	26.977706322564426	26.326439767270138	23.13158873218784
18	23.73991233353059	26.152258956486218	26.782263512628894	23.325565197354294
19	24.62286136233903	26.05317594566145	26.655424690103747	22.668538001895776
20	23.970652156836046	25.918795921466764	26.83107184565674	23.27948007604045
21	23.745149279134438	25.93733470890438	26.850029588742665	23.467486423218524
22	24.065231394441504	25.500259228807394	26.590277086791897	23.844232289959205
23	23.567826300988212	25.585516703238003	26.833690318458665	24.012966677315124
24	23.60427544239098	25.811124319851693	26.795565354462664	23.78903488329467
25	23.93556462129028	25.486747909149464	26.72308602730544	23.85460144225482
26	23.51514262821352	25.790490754172534	26.990379730925735	23.70398688668821
27	23.875863441406434	26.12397945022545	26.467732559661904	23.532424548706214
28	23.752481002979824	25.93115511309184	26.73691156369959	23.57945232022875
29	23.677906897581057	26.303816162261523	26.69847238296736	23.319804557190064
30	23.67403155783421	26.188393881152756	26.640342286764668	23.49723227424837
31	23.809249493325513	25.505600913323313	26.89464836528743	23.790501228063743
32	23.207524443443607	26.176244167351836	26.792108970364126	23.824122418840435
33	23.42904724248629	25.673497389382614	26.84364051510597	24.05381485302512
34	23.6831438431849	25.662395064702466	27.220595859670805	23.433865232441832
35	23.621871579619903	25.813638053741535	26.958120146006042	23.60637022063252
36	23.498279663369136	25.58321244717231	27.04128284219512	23.877225047263433
37	23.4403590449906	25.21599782143063	27.097003943420038	24.24663919015873
38	23.176626464380913	25.190336787971784	27.535336290461952	24.09770045718535
39	23.11105990542076	25.176406512665555	27.695796303763792	24.01673727814989
40	23.21412299490445	25.035008981361713	27.960052578933865	23.790815444799975
41	22.848793669580154	24.849516368073484	28.21865295285178	24.083037009494582
42	22.614911678912392	25.009662164639096	28.105011233248323	24.270414923200192
43	22.353483354368397	24.552267335599183	28.193306136129166	24.900943173903254
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	127.0
1	148.5
2	170.0
3	641.5
4	1113.0
5	1113.0
6	1379.0
7	1645.0
8	1464.0
9	1283.0
10	1632.5
11	1982.0
12	1982.0
13	3208.0
14	4434.0
15	7041.5
16	9649.0
17	7961.5
18	6274.0
19	6274.0
20	7174.5
21	8075.0
22	7444.5
23	6814.0
24	8282.0
25	9750.0
26	9750.0
27	11985.5
28	14221.0
29	16444.5
30	18668.0
31	21695.5
32	24723.0
33	24723.0
34	28189.5
35	31656.0
36	35980.5
37	40305.0
38	45124.0
39	49943.0
40	49943.0
41	54266.5
42	58590.0
43	62860.5
44	67131.0
45	71011.5
46	74892.0
47	74892.0
48	78406.0
49	81920.0
50	82805.0
51	83690.0
52	81460.5
53	79231.0
54	79231.0
55	75059.5
56	70888.0
57	65325.0
58	59762.0
59	54287.0
60	48812.0
61	48812.0
62	42431.0
63	36050.0
64	30237.5
65	24425.0
66	20002.5
67	15580.0
68	15580.0
69	12540.5
70	9501.0
71	7594.5
72	5688.0
73	4557.5
74	3427.0
75	3427.0
76	2640.0
77	1853.0
78	1496.0
79	1139.0
80	894.0
81	649.0
82	649.0
83	507.5
84	366.0
85	272.0
86	178.0
87	136.0
88	94.0
89	94.0
90	63.5
91	33.0
92	22.5
93	12.0
94	10.0
95	8.0
96	8.0
97	5.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	954755.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.93740102415649
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.01685203923867	60.15795075903768
2	7.670007676480342	10.72840805456729
3	2.4913551675747705	5.227167163448439
4	1.1374546401389858	3.1820248525675146
5	0.6825525078349584	2.3867974230248596
6	0.4433512692016511	1.8604101305234777
7	0.3047679992771195	1.4920277249341618
8	0.22207450749882787	1.2425051110550058
9	0.18035672609213044	1.1352312612098348
>10	0.8213649980941491	9.631674480275837
>50	0.02536245383602782	1.177219859106237
>100	0.0036000122148761382	0.39623161349807096
>500	3.0000083918292435E-4	0.14330213555305926
>1k	4.5000125877438647E-4	0.6436007332489155
>5k	1.5000041959146218E-4	0.5954486979496079
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5676	0.5944980649485994	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2424	0.2538871228744547	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2192	0.22958769527260922	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1519	0.15909840744484188	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	1.0473891207692026E-4
8	0.0	0.0	0.0	0.0	1.0473891207692026E-4
9	0.0	0.0	0.0	0.0	1.0473891207692026E-4
10	0.0	0.0	0.0	0.0	1.0473891207692026E-4
11	0.0	0.0	0.0	0.0	1.0473891207692026E-4
12	0.0	0.0	0.0	1.0473891207692026E-4	1.0473891207692026E-4
13	0.0	0.0	0.0	2.094778241538405E-4	1.0473891207692026E-4
14	0.0	0.0	0.0	2.094778241538405E-4	1.0473891207692026E-4
15	0.0	0.0	0.0	2.094778241538405E-4	1.0473891207692026E-4
16	0.0	0.0	0.0	2.094778241538405E-4	1.0473891207692026E-4
17	0.0	0.0	0.0	3.142167362307608E-4	1.0473891207692026E-4
18	0.0	0.0	0.0	3.142167362307608E-4	2.094778241538405E-4
19	0.0	0.0	0.0	4.18955648307681E-4	2.094778241538405E-4
20	0.0	1.0473891207692026E-4	0.0	9.426502086922823E-4	2.094778241538405E-4
21	0.0	1.0473891207692026E-4	0.0	0.001675822593230724	2.094778241538405E-4
22	0.0	1.0473891207692026E-4	0.0	0.003351645186461448	2.094778241538405E-4
23	0.0	1.0473891207692026E-4	0.0	0.00418955648307681	2.094778241538405E-4
24	0.0	1.0473891207692026E-4	0.0	0.0058653790763075345	2.094778241538405E-4
25	0.0	1.0473891207692026E-4	0.0	0.007122246021230578	2.094778241538405E-4
26	0.0	1.0473891207692026E-4	0.0	0.008483851878230541	2.094778241538405E-4
27	0.0	1.0473891207692026E-4	0.0	0.01623453137192264	2.094778241538405E-4
28	0.0	1.0473891207692026E-4	0.0	0.058758529675152264	2.094778241538405E-4
29	0.0	1.0473891207692026E-4	0.0	0.13794114720530398	2.094778241538405E-4
30	0.0	1.0473891207692026E-4	0.0	0.2378620693266859	2.094778241538405E-4
31	0.0	1.0473891207692026E-4	0.0	0.5538593670627543	2.094778241538405E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAAGAC	80	6.95758E-7	20.8125	3
TATACTG	185	0.0	18.0	5
TAGACTG	170	5.456968E-12	17.411764	5
CTTATAC	640	0.0	17.34375	37
GGGTAAG	225	0.0	17.266665	1
GCGACGT	65	0.0015796686	17.076925	33
GGCGAAA	65	0.0015796686	17.076925	10
CAGGACT	325	0.0	17.076923	4
GGTATCA	1355	0.0	16.247232	1
GTTACGG	70	0.0025920628	15.857142	25
GTACTAA	70	0.0025920628	15.857142	1
GCCTAAA	105	9.339703E-6	15.857142	1
TAGAGTG	165	9.731593E-10	15.69697	5
GCGAAAG	95	7.057675E-5	15.578948	11
ACCCGAC	215	0.0	15.488372	11
GTATTGG	205	5.456968E-12	15.341463	1
ATTAGAC	85	5.36297E-4	15.235294	3
GAATCCG	85	5.36297E-4	15.235294	5
CTAGTAC	85	5.36297E-4	15.235294	3
CCTACAC	220	1.8189894E-12	15.136364	3
>>END_MODULE