##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727393.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	832211
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.301685510044926	33.0	31.0	34.0	30.0	34.0
2	32.41442014104596	34.0	31.0	34.0	31.0	34.0
3	32.504924832764765	34.0	31.0	34.0	30.0	34.0
4	36.02554520428112	37.0	35.0	37.0	35.0	37.0
5	35.851562884893376	37.0	35.0	37.0	35.0	37.0
6	35.89219320580958	37.0	35.0	37.0	35.0	37.0
7	35.850333629331985	37.0	35.0	37.0	35.0	37.0
8	35.82572088088237	37.0	35.0	37.0	35.0	37.0
9	37.645297887194474	39.0	37.0	39.0	35.0	39.0
10	37.47753995080574	39.0	37.0	39.0	35.0	39.0
11	37.598535707891386	39.0	37.0	39.0	35.0	39.0
12	37.529899268334596	39.0	37.0	39.0	35.0	39.0
13	37.61344418663055	39.0	37.0	39.0	35.0	39.0
14	38.74101760250706	40.0	38.0	41.0	35.0	41.0
15	38.669456423911726	40.0	38.0	41.0	35.0	41.0
16	38.65487959183428	40.0	38.0	41.0	34.0	41.0
17	38.63771207061671	40.0	38.0	41.0	34.0	41.0
18	38.65029902272381	40.0	38.0	41.0	34.0	41.0
19	38.71652020941804	40.0	38.0	41.0	34.0	41.0
20	38.675003094167224	40.0	38.0	41.0	34.0	41.0
21	38.62502057771407	40.0	38.0	41.0	34.0	41.0
22	38.55714115771121	40.0	38.0	41.0	34.0	41.0
23	38.467184403955244	40.0	38.0	41.0	34.0	41.0
24	38.414257922570116	40.0	38.0	41.0	34.0	41.0
25	38.28825622348179	40.0	38.0	41.0	34.0	41.0
26	38.14105677526493	40.0	37.0	41.0	34.0	41.0
27	38.02200643827106	40.0	37.0	41.0	33.0	41.0
28	37.889388628605005	40.0	37.0	41.0	33.0	41.0
29	37.73046378863053	40.0	37.0	41.0	33.0	41.0
30	37.60093413809719	39.0	37.0	41.0	33.0	41.0
31	37.4208908558046	39.0	36.0	41.0	33.0	41.0
32	37.262206339498036	39.0	35.0	41.0	32.0	41.0
33	37.0929728157883	39.0	35.0	41.0	32.0	41.0
34	36.91341138244989	39.0	35.0	41.0	31.0	41.0
35	36.75226475016552	39.0	35.0	41.0	31.0	41.0
36	36.59302869104109	39.0	35.0	41.0	31.0	41.0
37	36.423936958295435	39.0	35.0	41.0	30.0	41.0
38	36.2597886833988	39.0	35.0	40.0	30.0	41.0
39	36.0693045393536	39.0	35.0	40.0	29.0	41.0
40	35.85366211213262	39.0	35.0	40.0	28.0	41.0
41	35.64382590472849	39.0	35.0	40.0	26.0	41.0
42	35.41942848628533	39.0	35.0	40.0	25.0	41.0
43	33.83321657608467	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	4.0
13	4.0
14	5.0
15	6.0
16	11.0
17	21.0
18	27.0
19	66.0
20	95.0
21	276.0
22	498.0
23	871.0
24	1477.0
25	2330.0
26	3835.0
27	5718.0
28	8225.0
29	11247.0
30	14474.0
31	18102.0
32	22645.0
33	29178.0
34	38291.0
35	51518.0
36	76376.0
37	129087.0
38	205400.0
39	212419.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.63878631741229	17.678209011897223	13.563267008006383	23.119737662684102
2	16.098801866353604	24.262837189126316	36.136748973517534	23.501611971002546
3	19.144183386184512	24.952926601546963	31.430130099217624	24.472759913050897
4	12.83304354304377	15.319191887634265	35.03510527979083	36.81265928953114
5	14.43612256987711	38.95418349433017	33.43707304998372	13.172620885809009
6	33.574778511699556	36.439196309589754	15.096892494812012	14.889132683898676
7	27.99374197168747	31.175026525724846	22.338685741957267	18.492545760630417
8	28.89615734471186	35.21835207657673	19.59406929252317	16.29142128618824
9	26.304266586238345	13.916422638008871	19.83271069476371	39.94660008098908
10	14.844552643500267	29.45082437026187	33.81546266511738	21.889160321120485
11	35.032461719443745	22.64581938955385	22.03191257986256	20.289806311139845
12	20.537459850927227	27.259553166204242	31.907653227366616	20.29533375550191
13	32.17224958574208	19.35855209796554	25.87552916267629	22.593669153616087
14	20.9378390816752	20.798090868782076	30.353119581452297	27.910950468090423
15	24.166587560125976	29.621574336316154	24.448727546259303	21.76311055729857
16	21.7575831129365	26.76676948514259	26.9357170236875	24.539930378233404
17	22.253370839847104	27.549023024209006	28.850495847807828	21.34711028813606
18	21.911149936734795	25.715834085346145	29.93952254896895	22.43349342895011
19	23.03213968572874	26.767850941648213	29.938921739799163	20.261087632823887
20	21.88291190575467	27.22074089383582	29.70869166593568	21.18765553447383
21	21.322477112174674	27.88884069064216	29.814554241652658	20.97412795553051
22	21.739678999676766	27.52547130475324	29.04924352117432	21.685606174395677
23	21.250139688131974	27.073302323569383	29.400116076331607	22.276441911967037
24	21.077587294568325	27.40867400214609	29.79809207040042	21.715646632885168
25	21.374867671780354	27.232396591729742	29.66014628501666	21.732589451473245
26	21.058601724802966	27.699225316656474	30.229112568807672	21.013060389732892
27	21.68836989657671	28.06848263240933	29.755554781179296	20.48759268983467
28	21.235359782555147	28.210754243815572	29.61869045230116	20.935195521328126
29	20.88989450992597	28.43978269933947	29.844835023810067	20.825487766924493
30	20.513427484135637	28.77239065573514	29.87439483496373	20.839787025165492
31	20.744018043500986	27.75449976027714	29.843272919968616	21.658209276253256
32	20.317563694784134	28.452760177406933	29.73020063421416	21.499475493594776
33	20.913446229381734	27.949041769455103	29.557528078816553	21.579983922346617
34	20.99852080782398	28.0333953768936	29.98410258936736	20.983981225915063
35	20.91464784772131	28.290541701563665	29.612442036935345	21.182368413779677
36	20.609917436803887	27.856517157307465	30.05968438292693	21.473881022961724
37	20.34171622340969	27.73491338134199	29.78283151748775	22.14053887776057
38	20.233450411013553	27.623403199428992	30.46042409917677	21.682722290380685
39	20.109924045704755	27.421411156545634	30.640066040943942	21.82859875680567
40	20.317563694784134	27.473801716151314	30.82535558890714	21.383279000157412
41	19.937131328473185	27.214372316636045	30.94996341072156	21.898532944169208
42	19.305560729190073	27.466231520611963	30.787144125708505	22.441063624489463
43	18.936904222607005	26.84271176420403	30.920043114066026	23.30034089912294
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	401.0
1	415.0
2	429.0
3	1918.0
4	3407.0
5	3407.0
6	4361.5
7	5316.0
8	4742.5
9	4169.0
10	5420.0
11	6671.0
12	6671.0
13	10774.0
14	14877.0
15	23223.5
16	31570.0
17	24498.0
18	17426.0
19	17426.0
20	17917.0
21	18408.0
22	14034.5
23	9661.0
24	11012.0
25	12363.0
26	12363.0
27	13991.5
28	15620.0
29	17230.5
30	18841.0
31	20084.5
32	21328.0
33	21328.0
34	23285.5
35	25243.0
36	27866.0
37	30489.0
38	33805.0
39	37121.0
40	37121.0
41	40972.0
42	44823.0
43	48243.0
44	51663.0
45	54850.0
46	58037.0
47	58037.0
48	62064.0
49	66091.0
50	65900.5
51	65710.0
52	64653.5
53	63597.0
54	63597.0
55	58358.5
56	53120.0
57	48385.0
58	43650.0
59	39730.5
60	35811.0
61	35811.0
62	30865.0
63	25919.0
64	21758.5
65	17598.0
66	14662.5
67	11727.0
68	11727.0
69	9529.5
70	7332.0
71	6091.5
72	4851.0
73	4019.0
74	3187.0
75	3187.0
76	2651.5
77	2116.0
78	1767.5
79	1419.0
80	1190.0
81	961.0
82	961.0
83	770.5
84	580.0
85	473.5
86	367.0
87	274.5
88	182.0
89	182.0
90	136.5
91	91.0
92	54.5
93	18.0
94	16.5
95	15.0
96	15.0
97	10.5
98	6.0
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	832211.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.2715455368551
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.03707555268757	56.81044440331945
2	7.277350160437754	9.500077847693053
3	2.2320076499901944	4.370597668948318
4	1.0698342435158252	2.7931893817012052
5	0.621240065594428	2.027464961538278
6	0.3871703909411746	1.5162725881703323
7	0.25656019762900845	1.1722256435740461
8	0.18551019212375897	0.9686829562205337
9	0.14886527115090176	0.8744999692304131
>10	0.7091557423955859	8.225363041839934
>50	0.0425946356011965	1.9241094275583621
>100	0.02931699307356683	3.625424241231831
>500	0.0016594524295735645	0.7265531165082125
>1k	9.21918016429758E-4	1.18460532148258
>5k	5.531508098578548E-4	2.09553468024532
>10k+	1.843836032859516E-4	2.184954750738214
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	18155	2.1815380955070287	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6187	0.7434412666979888	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6178	0.7423598101923671	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5047	0.6064567759858978	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	2461	0.2957182733705755	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2393	0.2875472686614332	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1972	0.2369591365651259	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1726	0.20739932541146416	No Hit
GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	1291	0.1551289276397452	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	861	0.10345933903781614	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.201618339579746E-4	0.0
5	0.0	0.0	0.0	1.201618339579746E-4	0.0
6	0.0	0.0	0.0	1.201618339579746E-4	0.0
7	0.0	0.0	0.0	1.201618339579746E-4	0.0
8	0.0	0.0	0.0	2.403236679159492E-4	0.0
9	0.0	0.0	0.0	2.403236679159492E-4	0.0
10	0.0	0.0	0.0	3.604855018739238E-4	0.0
11	0.0	0.0	0.0	7.209710037478476E-4	0.0
12	1.201618339579746E-4	0.0	0.0	0.0015621038414536698	0.0
13	1.201618339579746E-4	0.0	0.0	0.001802427509369619	0.0
14	1.201618339579746E-4	0.0	0.0	0.0020427511772855684	1.201618339579746E-4
15	1.201618339579746E-4	0.0	0.0	0.002763722181033416	2.403236679159492E-4
16	2.403236679159492E-4	0.0	0.0	0.004085502354571137	3.604855018739238E-4
17	2.403236679159492E-4	0.0	0.0	0.005407282528108857	3.604855018739238E-4
18	2.403236679159492E-4	0.0	0.0	0.006248415365814679	3.604855018739238E-4
19	2.403236679159492E-4	0.0	0.0	0.007329871871436451	3.604855018739238E-4
20	2.403236679159492E-4	0.0	0.0	0.011535536059965561	3.604855018739238E-4
21	2.403236679159492E-4	0.0	0.0	0.01742346592390632	3.604855018739238E-4
22	2.403236679159492E-4	0.0	0.0	0.031963047832821244	3.604855018739238E-4
23	2.403236679159492E-4	0.0	0.0	0.03953324337217364	3.604855018739238E-4
24	2.403236679159492E-4	0.0	0.0	0.0525107214396349	3.604855018739238E-4
25	2.403236679159492E-4	0.0	0.0	0.06224382999023084	3.604855018739238E-4
26	2.403236679159492E-4	0.0	0.0	0.07173661487291083	3.604855018739238E-4
27	2.403236679159492E-4	0.0	0.0	0.08903991896285918	3.604855018739238E-4
28	2.403236679159492E-4	0.0	0.0	0.1906968304913057	3.604855018739238E-4
29	2.403236679159492E-4	0.0	0.0	0.40085987808380324	3.604855018739238E-4
30	2.403236679159492E-4	0.0	0.0	0.6403424131620467	3.604855018739238E-4
31	2.403236679159492E-4	0.0	0.0	1.2151966268169971	3.604855018739238E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTACG	20	0.0018416442	37.0	1
CGAACCG	25	0.0054954826	29.6	18
ATTGCGA	35	8.86733E-4	26.42857	10
GTATTCG	40	0.0019307417	23.125	5
GTAATAC	50	2.7012342E-4	22.2	3
CGCTATC	50	2.7012342E-4	22.2	33
ACCGCTA	85	5.17648E-8	21.764706	8
TAGTACA	60	3.724696E-5	21.583334	4
ACCGTGA	125	1.8189894E-11	20.72	8
CCGCTAC	90	9.4703864E-8	20.555555	9
AACCGTC	60	9.2338957E-4	18.5	7
TAATAGT	80	1.6157968E-5	18.5	4
GGTCACG	50	0.007033291	18.5	11
TATTCGT	50	0.007033291	18.5	6
GGCGATC	50	0.007033291	18.5	34
CTAGCAC	80	1.6157968E-5	18.5	3
TAATACC	75	2.0664027E-4	17.266666	4
CCTAGAC	75	2.0664027E-4	17.266666	3
CACGATC	130	1.391345E-8	17.076923	26
ACGATCC	120	1.0401527E-7	16.958334	27
>>END_MODULE