FastQCFastQC Report
Thu 9 Feb 2017
SRR2727392.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727392.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4402135
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT464351.0548290772545594No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT177970.4042811045095164No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT174330.39601238944284983No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125710.2855659810523757No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61460.1396140736256385No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53430.12137292472856921No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA50600.11494422592673782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG1603.45608E-1117.343755
CTTATAC61000.017.01393537
TATACAC24250.015.71546437
AACGCAG192150.015.3468655
CGGTGAT7850.015.31847114
AGTCGGT8150.014.75460111
ACGGATG10950.014.3607328
TACGGAT10050.014.17412927
ACGCAGA215150.013.8180346
CGCAGAG216300.013.7873327
TCGGTGA9000.013.772222513
ATCGAAT1356.576609E-613.70370428
TCTATAC5200.013.519233
TGTTACG5650.013.42477924
AGAGTAC225350.013.20900910
TATACTG6500.013.0923075
TTATACT7550.012.9867554
CAGAGTA231050.012.9712189
AGTGTAC13150.012.942965527
GGCGTCA7450.012.91275112
Produced by FastQC (version 0.11.5)