FastQCFastQC Report
Thu 9 Feb 2017
SRR2727391.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727391.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7316270
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT576600.7881065078243422No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT236470.3232111444766254No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT223990.3061532720908332No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148890.2035053381026124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC91350.018.14559637
TAGATCG2050.017.1463415
CGTTACG8850.017.14124324
TCGTTAC11250.016.93777723
AACGCAG239100.016.0163125
ACGGATG25900.015.85714228
TACGGAT25400.015.73228427
CTCGTTA14200.015.24295822
GTTACGG18950.014.93667525
TTACGGA27650.014.85352626
CGGATGG29050.014.6471629
ACGCAGA268550.014.3150246
CGCAGAG271750.014.1532667
CATTACG14100.013.90780224
TACTTAC22600.013.83407131
GGAGTGT30900.013.8300986
ATCTCGT17450.013.67621820
TATACAC34250.013.66569337
GTATAAG10300.013.6504861
ATTACGG14050.013.56227825
Produced by FastQC (version 0.11.5)