##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727391.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7316270
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34456574183293	33.0	31.0	34.0	31.0	34.0
2	32.48668023460042	34.0	31.0	34.0	31.0	34.0
3	32.577591040243185	34.0	31.0	34.0	31.0	34.0
4	36.10769080419394	37.0	35.0	37.0	35.0	37.0
5	35.91634576088635	37.0	35.0	37.0	35.0	37.0
6	35.9310491548289	37.0	35.0	37.0	35.0	37.0
7	35.89302486102891	37.0	35.0	37.0	35.0	37.0
8	35.84856723439676	37.0	35.0	37.0	35.0	37.0
9	37.687906542541484	39.0	37.0	39.0	35.0	39.0
10	37.513146863087336	39.0	37.0	39.0	35.0	39.0
11	37.6259587194021	39.0	37.0	39.0	35.0	39.0
12	37.550876471207324	39.0	37.0	39.0	35.0	39.0
13	37.62795099142049	39.0	37.0	39.0	35.0	39.0
14	38.85414001943613	40.0	38.0	41.0	36.0	41.0
15	38.77905886469471	40.0	38.0	41.0	35.0	41.0
16	38.74648775947306	40.0	38.0	41.0	35.0	41.0
17	38.69410751653506	40.0	38.0	41.0	35.0	41.0
18	38.65918152282516	40.0	38.0	41.0	35.0	41.0
19	38.71931161643843	40.0	38.0	41.0	35.0	41.0
20	38.67788053201973	40.0	38.0	41.0	34.0	41.0
21	38.63484617161477	40.0	38.0	41.0	34.0	41.0
22	38.59949113414349	40.0	38.0	41.0	34.0	41.0
23	38.53103070280348	40.0	38.0	41.0	34.0	41.0
24	38.48902090819502	40.0	38.0	41.0	34.0	41.0
25	38.37375329231972	40.0	38.0	41.0	34.0	41.0
26	38.26202873868788	40.0	38.0	41.0	34.0	41.0
27	38.171250650946455	40.0	38.0	41.0	34.0	41.0
28	38.07364927756903	40.0	38.0	41.0	33.0	41.0
29	37.98930985324489	40.0	37.0	41.0	33.0	41.0
30	37.92483382925999	40.0	37.0	41.0	33.0	41.0
31	37.820072933338984	40.0	37.0	41.0	33.0	41.0
32	37.72005899180867	40.0	37.0	41.0	33.0	41.0
33	37.61989442707828	40.0	37.0	41.0	33.0	41.0
34	37.51725360053688	40.0	37.0	41.0	32.0	41.0
35	37.450597230556006	40.0	37.0	41.0	32.0	41.0
36	37.35033275152502	40.0	36.0	41.0	32.0	41.0
37	37.27277027775082	40.0	36.0	41.0	32.0	41.0
38	37.17881229096247	39.0	36.0	41.0	31.0	41.0
39	37.061464926800134	39.0	36.0	41.0	31.0	41.0
40	36.94773853343302	39.0	36.0	41.0	31.0	41.0
41	36.832776537771295	39.0	36.0	41.0	31.0	41.0
42	36.71967778663171	39.0	36.0	41.0	30.0	41.0
43	35.01902554170363	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	8.0
10	19.0
11	19.0
12	24.0
13	25.0
14	18.0
15	24.0
16	46.0
17	84.0
18	180.0
19	364.0
20	837.0
21	1649.0
22	3214.0
23	5704.0
24	10097.0
25	15616.0
26	24268.0
27	36374.0
28	52335.0
29	74189.0
30	100052.0
31	130852.0
32	169815.0
33	223327.0
34	304153.0
35	419175.0
36	619801.0
37	1040476.0
38	1998338.0
39	2085184.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.950305278509404	17.36004822129309	12.470712535212614	26.218933964984892
2	16.650615682581424	20.743958328492525	37.152879814440965	25.452546174485086
3	18.27282208010366	22.471532625231163	31.17438257472729	28.081262719937893
4	13.374123699644763	15.071012961522742	35.43654075095643	36.118322587876065
5	14.172686355205592	37.38928443045432	34.77744807121662	13.660581143123476
6	34.856409071835785	36.19829503285144	14.999719802576998	13.945576092735779
7	29.44310420473821	31.424482694050383	21.120338642504993	18.01207445870642
8	27.531214676330972	36.41268023186679	19.160979570190822	16.895125521611423
9	27.22261480235147	13.529380408322819	18.49130772921174	40.756697060113964
10	14.75270868899043	27.853168349445824	34.5450892326281	22.849033728935648
11	36.941282921488686	21.196374655391338	21.874698446066095	19.98764397705388
12	21.012797504739435	25.120737752980688	31.44364546415045	22.42281927812943
13	31.45396493021717	19.259322031581664	24.798961766036516	24.48775127216464
14	21.519818158706556	19.563766782800528	27.53362027371871	31.38279478477421
15	25.116787652724682	28.19234391295018	22.64648516252134	24.044383271803802
16	23.822056867775522	26.526508726441207	24.985340890918458	24.666093514864816
17	23.682586891954507	26.601041240960217	26.175592207504643	23.540779659580632
18	23.59373013844486	26.10218048267765	26.840329840205463	23.463759538672026
19	24.880273691375525	25.855770768438013	26.75124072785723	22.512714812329232
20	23.851334081437674	25.662393542064464	26.793953749656584	23.69231862684127
21	23.552944328189092	26.07876691264811	27.189483165602148	23.178805593560654
22	24.26916994588773	25.275912452656886	26.323317756179037	24.131599845276348
23	23.364815677934246	25.529593631727643	27.00477155709125	24.10081913324686
24	23.313901755949413	25.36696978104963	27.154684012481773	24.164444450519184
25	23.783293946232163	25.247619347016993	26.784960095786513	24.18412661096433
26	23.314257128290784	25.6264599310851	27.266065905167526	23.793217035456593
27	23.994439789674246	26.118937655389974	26.569973497424233	23.316649057511547
28	23.5604481518588	26.392806717083978	26.344312060653856	23.702433070403362
29	23.410850064308725	26.443050898886998	26.833564097552443	23.31253493925183
30	24.023347962828055	26.016385398570584	26.441902772861038	23.51836386574033
31	23.582153201016364	25.522882561742527	26.9243343944387	23.970629842802413
32	23.013311427817726	26.079504993664806	26.925209157125146	23.981974421392323
33	23.370064254052952	25.29716645230425	26.97866535816748	24.35410393547532
34	23.580321666641606	25.434340176073327	27.672133477851418	23.313204679433646
35	23.49352880634531	25.37828702330559	27.12397164128716	24.00421252906194
36	23.097274977550036	25.140023536583534	27.7216669149717	24.04103457089473
37	22.868469862375225	24.844736457238454	27.75134050547615	24.535453174910167
38	23.000189987520965	24.636447260694318	28.086196928216157	24.277165823568566
39	22.419128873045967	24.63277052377783	28.773432363759127	24.17466823941708
40	23.002035190062696	24.38604644169775	28.726085833354976	23.885832534884578
41	22.23590709473543	24.495118414164597	29.07985899918948	24.189115491910496
42	21.857626905513328	24.714096117283805	29.015686955238117	24.412590021964746
43	21.363085287995112	24.17054045299039	28.79573881226363	25.67063544675087
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	860.0
1	1234.5
2	1609.0
3	6428.0
4	11247.0
5	11247.0
6	13952.5
7	16658.0
8	15073.0
9	13488.0
10	17594.5
11	21701.0
12	21701.0
13	33627.0
14	45553.0
15	72730.0
16	99907.0
17	80740.5
18	61574.0
19	61574.0
20	66908.0
21	72242.0
22	62377.5
23	52513.0
24	62673.5
25	72834.0
26	72834.0
27	87381.5
28	101929.0
29	117056.5
30	132184.0
31	150930.0
32	169676.0
33	169676.0
34	194083.5
35	218491.0
36	248440.5
37	278390.0
38	312278.5
39	346167.0
40	346167.0
41	381582.0
42	416997.0
43	452398.5
44	487800.0
45	527423.0
46	567046.0
47	567046.0
48	599818.0
49	632590.0
50	643909.5
51	655229.0
52	640569.5
53	625910.0
54	625910.0
55	587405.0
56	548900.0
57	506823.5
58	464747.0
59	423790.0
60	382833.0
61	382833.0
62	333970.0
63	285107.0
64	240263.0
65	195419.0
66	162118.0
67	128817.0
68	128817.0
69	104803.5
70	80790.0
71	66283.5
72	51777.0
73	41628.0
74	31479.0
75	31479.0
76	25261.0
77	19043.0
78	15123.5
79	11204.0
80	8812.0
81	6420.0
82	6420.0
83	5109.5
84	3799.0
85	2872.5
86	1946.0
87	1417.0
88	888.0
89	888.0
90	602.0
91	316.0
92	214.0
93	112.0
94	84.0
95	56.0
96	56.0
97	36.5
98	17.0
99	11.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7316270.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.61814787674068
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.4392783250874	40.21069137480835
2	10.257784233131417	10.384600784006901
3	3.5311638205689095	5.36222917339661
4	1.8191553325051721	3.6832909452603264
5	1.070507202026655	2.7093545927650564
6	0.7140228383985189	2.1685508172855803
7	0.5156858440123924	1.8272143617112773
8	0.36627937161921953	1.4832306717457047
9	0.29833092344803097	1.359086291936733
>10	1.776996372612474	16.906704496805926
>50	0.14447198077016243	5.028481305178072
>100	0.06311628103898201	5.647487796818741
>500	0.002210257234574351	0.7501778922417391
>1k	8.085547706379384E-4	0.6185789101270036
>5k	8.085547523440302E-5	0.2423865582046167
>10k+	1.0780730031253735E-4	1.6179340277073517
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	57660	0.7881065078243422	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23647	0.3232111444766254	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	22399	0.3061532720908332	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14889	0.2035053381026124	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3668166975795043E-5	0.0	0.0	0.0	1.3668166975795043E-5
2	1.3668166975795043E-5	1.3668166975795043E-5	0.0	0.0	1.3668166975795043E-5
3	1.3668166975795043E-5	1.3668166975795043E-5	0.0	0.0	1.3668166975795043E-5
4	1.3668166975795043E-5	1.3668166975795043E-5	0.0	0.0	1.3668166975795043E-5
5	1.3668166975795043E-5	1.3668166975795043E-5	0.0	0.0	1.3668166975795043E-5
6	1.3668166975795043E-5	1.3668166975795043E-5	0.0	0.0	4.100450092738513E-5
7	1.3668166975795043E-5	1.3668166975795043E-5	0.0	1.3668166975795043E-5	4.100450092738513E-5
8	1.3668166975795043E-5	1.3668166975795043E-5	0.0	1.3668166975795043E-5	5.467266790318017E-5
9	1.3668166975795043E-5	1.3668166975795043E-5	0.0	2.7336333951590086E-5	5.467266790318017E-5
10	2.7336333951590086E-5	1.3668166975795043E-5	0.0	2.7336333951590086E-5	5.467266790318017E-5
11	4.100450092738513E-5	1.3668166975795043E-5	0.0	1.230135027821554E-4	5.467266790318017E-5
12	4.100450092738513E-5	1.3668166975795043E-5	0.0	1.913543376611306E-4	5.467266790318017E-5
13	4.100450092738513E-5	1.3668166975795043E-5	0.0	2.0502250463692563E-4	5.467266790318017E-5
14	4.100450092738513E-5	1.3668166975795043E-5	0.0	2.870315064916959E-4	9.56771688305653E-5
15	5.467266790318017E-5	1.3668166975795043E-5	0.0	4.1004500927385127E-4	1.7768617068533555E-4
16	6.834083487897521E-5	1.3668166975795043E-5	0.0	6.560720148381621E-4	2.0502250463692563E-4
17	6.834083487897521E-5	1.3668166975795043E-5	0.0	9.841080222572432E-4	2.0502250463692563E-4
18	8.200900185477026E-5	1.3668166975795043E-5	0.0	0.0010251125231846283	2.3235883858851574E-4
19	9.56771688305653E-5	1.3668166975795043E-5	1.3668166975795043E-5	0.0014351575324584795	2.3235883858851574E-4
20	9.56771688305653E-5	1.3668166975795043E-5	1.3668166975795043E-5	0.002227911217054592	2.3235883858851574E-4
21	1.0934533580636034E-4	1.3668166975795043E-5	1.3668166975795043E-5	0.003813418586246817	2.7336333951590084E-4
22	1.0934533580636034E-4	1.3668166975795043E-5	1.3668166975795043E-5	0.005822639131688689	2.7336333951590084E-4
23	1.0934533580636034E-4	1.3668166975795043E-5	1.3668166975795043E-5	0.007148451328340808	3.2803600741908106E-4
24	1.0934533580636034E-4	1.3668166975795043E-5	1.3668166975795043E-5	0.010292129732773667	3.2803600741908106E-4
25	1.0934533580636034E-4	1.3668166975795043E-5	1.3668166975795043E-5	0.012520040949828259	3.2803600741908106E-4
26	1.0934533580636034E-4	1.3668166975795043E-5	1.3668166975795043E-5	0.015609046686357939	3.2803600741908106E-4
27	1.0934533580636034E-4	1.3668166975795043E-5	1.3668166975795043E-5	0.030534685023926127	3.4170417439487606E-4
28	1.0934533580636034E-4	1.3668166975795043E-5	1.3668166975795043E-5	0.12377892013279991	3.4170417439487606E-4
29	1.0934533580636034E-4	1.3668166975795043E-5	1.3668166975795043E-5	0.2892047450408473	3.4170417439487606E-4
30	1.230135027821554E-4	1.3668166975795043E-5	1.3668166975795043E-5	0.4888556600562855	3.4170417439487606E-4
31	1.230135027821554E-4	1.3668166975795043E-5	1.3668166975795043E-5	1.1020506350913786	3.4170417439487606E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	9135	0.0	18.145596	37
TAGATCG	205	0.0	17.146341	5
CGTTACG	885	0.0	17.141243	24
TCGTTAC	1125	0.0	16.937777	23
AACGCAG	23910	0.0	16.016312	5
ACGGATG	2590	0.0	15.857142	28
TACGGAT	2540	0.0	15.732284	27
CTCGTTA	1420	0.0	15.242958	22
GTTACGG	1895	0.0	14.936675	25
TTACGGA	2765	0.0	14.853526	26
CGGATGG	2905	0.0	14.64716	29
ACGCAGA	26855	0.0	14.315024	6
CGCAGAG	27175	0.0	14.153266	7
CATTACG	1410	0.0	13.907802	24
TACTTAC	2260	0.0	13.834071	31
GGAGTGT	3090	0.0	13.830098	6
ATCTCGT	1745	0.0	13.676218	20
TATACAC	3425	0.0	13.665693	37
GTATAAG	1030	0.0	13.650486	1
ATTACGG	1405	0.0	13.562278	25
>>END_MODULE