FastQCFastQC Report
Thu 9 Feb 2017
SRR2727390.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727390.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4182122
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT417250.9976992541107121No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT165550.39585167529785115No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT150670.36027165156827085No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108990.26060932703541406No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47940.11463080225780119No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA44590.10662051465739164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC53200.016.865637
AACGCAG147800.015.6586615
TATACAC20200.015.38613837
TAGATCG1252.9630828E-614.7999995
GTATAGA4850.014.4948451
CGCAATC2301.8189894E-1214.47826122
TATACGA1051.6572804E-414.0952382
ACGCAGA167300.013.9772866
CTAATAC4650.013.9247313
CGCAGAG169000.013.8695277
TCGTTAC4450.013.3033723
TATACAG6400.013.2968755
TAGTACT5050.013.1881184
AGAGTAC178200.013.15348110
CGAACCG1551.890714E-613.12903218
TAAGAGT6350.013.1102364
TAATACG1703.7381506E-713.0588234
CAGAGTA180800.013.0359529
AACGGTA1857.3983756E-813.018
TATACTG6750.012.8814815
Produced by FastQC (version 0.11.5)