##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727390.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4182122
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30315710541204	33.0	31.0	34.0	30.0	34.0
2	32.44573855090789	34.0	31.0	34.0	31.0	34.0
3	32.53376204711388	34.0	31.0	34.0	31.0	34.0
4	36.07614388102499	37.0	35.0	37.0	35.0	37.0
5	35.884137048130114	37.0	35.0	37.0	35.0	37.0
6	35.89966481130871	37.0	35.0	37.0	35.0	37.0
7	35.863184287785	37.0	35.0	37.0	35.0	37.0
8	35.81942468440663	37.0	35.0	37.0	35.0	37.0
9	37.65271720911059	39.0	37.0	39.0	35.0	39.0
10	37.482095213865115	39.0	37.0	39.0	35.0	39.0
11	37.58937831081924	39.0	37.0	39.0	35.0	39.0
12	37.51157976740038	39.0	37.0	39.0	35.0	39.0
13	37.59552519032204	39.0	37.0	39.0	35.0	39.0
14	38.80362050652755	40.0	38.0	41.0	35.0	41.0
15	38.73003489616037	40.0	38.0	41.0	35.0	41.0
16	38.70065053099838	40.0	38.0	41.0	35.0	41.0
17	38.6458305137918	40.0	38.0	41.0	34.0	41.0
18	38.618585254088714	40.0	38.0	41.0	34.0	41.0
19	38.66970762689372	40.0	38.0	41.0	34.0	41.0
20	38.62999907702358	40.0	38.0	41.0	34.0	41.0
21	38.587084738321835	40.0	38.0	41.0	34.0	41.0
22	38.54328424660974	40.0	38.0	41.0	34.0	41.0
23	38.47279658508288	40.0	38.0	41.0	34.0	41.0
24	38.4287139399568	40.0	38.0	41.0	34.0	41.0
25	38.31012844675502	40.0	38.0	41.0	34.0	41.0
26	38.1933855588144	40.0	38.0	41.0	34.0	41.0
27	38.08928673051623	40.0	38.0	41.0	33.0	41.0
28	37.99407406096714	40.0	37.0	41.0	33.0	41.0
29	37.89763689342396	40.0	37.0	41.0	33.0	41.0
30	37.81860117901869	40.0	37.0	41.0	33.0	41.0
31	37.70522165541799	40.0	37.0	41.0	33.0	41.0
32	37.59686948396053	40.0	37.0	41.0	33.0	41.0
33	37.48303516731458	40.0	36.0	41.0	32.0	41.0
34	37.376330963085245	40.0	36.0	41.0	32.0	41.0
35	37.28740314127613	39.0	36.0	41.0	32.0	41.0
36	37.18364337530086	39.0	36.0	41.0	31.0	41.0
37	37.08116836381148	39.0	36.0	41.0	31.0	41.0
38	36.971057994004	39.0	36.0	41.0	31.0	41.0
39	36.844755604929745	39.0	36.0	41.0	31.0	41.0
40	36.70982386453576	39.0	35.0	41.0	30.0	41.0
41	36.57632919364858	39.0	35.0	41.0	30.0	41.0
42	36.43805871756013	39.0	35.0	40.0	30.0	41.0
43	34.7466977290476	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	18.0
11	25.0
12	14.0
13	13.0
14	15.0
15	18.0
16	27.0
17	59.0
18	116.0
19	267.0
20	555.0
21	1042.0
22	2024.0
23	3615.0
24	6195.0
25	10045.0
26	15580.0
27	23028.0
28	33309.0
29	46586.0
30	61948.0
31	80006.0
32	102411.0
33	132914.0
34	178864.0
35	244942.0
36	362747.0
37	606288.0
38	1122959.0
39	1146489.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.10892365167731	17.5726341794907	12.719595458956004	25.59884670987599
2	16.86079459183639	21.177885293638017	36.70105750143109	25.260262613094504
3	18.33090952392111	22.59259772909542	31.157101586228237	27.919391160755236
4	13.318119366197351	15.150227563901769	35.377710167230894	36.15394290266998
5	14.284470897788252	37.36863726117985	34.61195058393801	13.734941257093885
6	34.661638278366816	36.3565194893884	14.913816478811473	14.068025753433306
7	29.34751783903004	31.16857901323778	21.264587690172597	18.219315457559585
8	27.588817351574153	36.060856187361345	19.195566269946212	17.15476019111829
9	26.65398092164695	13.626551305772525	18.82006311628403	40.8994046562965
10	15.040044264610167	27.80239313917671	34.26970805729723	22.88785453891589
11	36.51646221702762	21.295170250891772	22.004212215712503	20.184155316368102
12	20.967824468057124	25.57338116869857	31.400829531037118	22.05796483220719
13	31.608427492072206	19.13093400909873	24.933060298097473	24.32757820073159
14	21.447270070074474	19.657174037486232	27.768821665173803	31.12673422726549
15	24.77742160558683	28.41060112545736	22.72879652960865	24.083180739347153
16	23.783309047416598	26.420223991552618	24.98281972644509	24.813647234585694
17	23.640773750741847	26.770261604037376	26.428306013071833	23.16065863214894
18	23.578006571783416	25.735643293045968	27.07455688762786	23.611793247542753
19	24.620396057312533	25.791810951473913	27.176753810625325	22.411039180588226
20	23.851241068529326	25.80324055587092	27.04471557740305	23.300802798196703
21	23.518395685252607	26.1199218961092	27.2822744051943	23.079408013443892
22	23.971921431273408	25.557838819623147	26.7359488795401	23.734290869563345
23	23.17127525213277	25.670676273910708	27.116951633644355	24.041096840312168
24	23.316440792497207	25.67698885876596	27.31225918325673	23.69431116548011
25	23.71353585572109	25.463221780713237	27.04485904524067	23.778383318325
26	23.31194546691847	25.85553458268314	27.410630297251014	23.421889653147375
27	23.73749498460351	26.221114544243328	26.891468015519393	23.149922455633767
28	23.36120275783442	26.296626449443604	26.944216357150747	23.397954435571226
29	23.25489309015854	26.44114638453876	27.152794681742904	23.151165843559802
30	23.414429325591172	26.339762445954467	26.969777543553246	23.276030684901112
31	23.322203417308245	25.67050889476682	27.23493001877994	23.772357669144995
32	22.75701187100711	26.245504076638603	27.298055867332423	23.699428185021862
33	23.186578488145493	25.661111751402753	27.258697857212198	23.89361190323955
34	23.292792510596293	25.687151163930654	27.714949492147767	23.305106833325283
35	23.21639588706403	25.738440915879547	27.409817312837838	23.635345884218587
36	22.980319560261513	25.50791201213164	27.773890862103016	23.73787756550383
37	22.772171639182215	25.174636225342063	27.80150842084473	24.251683714630996
38	22.641711552173753	25.089105482814706	28.271939460398332	23.99724350461321
39	22.3254845267546	24.96318854399752	28.75975401961014	23.95157290963774
40	22.528921920498732	24.943963853756536	28.821445189786427	23.7056690359583
41	22.037711955796603	24.808243279368703	29.128705475354376	24.025339289480314
42	21.62043575008094	25.055438363586713	29.01393120525896	24.310194681073387
43	21.323624705352927	24.45210828378512	28.964817382180623	25.259449628681324
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	629.0
1	806.5
2	984.0
3	4272.5
4	7561.0
5	7561.0
6	9723.0
7	11885.0
8	10781.0
9	9677.0
10	12827.0
11	15977.0
12	15977.0
13	25043.0
14	34109.0
15	53337.0
16	72565.0
17	56973.0
18	41381.0
19	41381.0
20	44165.5
21	46950.0
22	38197.0
23	29444.0
24	35216.5
25	40989.0
26	40989.0
27	49449.5
28	57910.0
29	65823.0
30	73736.0
31	83518.5
32	93301.0
33	93301.0
34	107191.5
35	121082.0
36	139020.0
37	156958.0
38	176783.5
39	196609.0
40	196609.0
41	216420.5
42	236232.0
43	257719.0
44	279206.0
45	298896.0
46	318586.0
47	318586.0
48	336420.5
49	354255.0
50	359020.5
51	363786.0
52	357705.5
53	351625.0
54	351625.0
55	332817.5
56	314010.0
57	290970.0
58	267930.0
59	241729.5
60	215529.0
61	215529.0
62	188669.5
63	161810.0
64	137161.5
65	112513.0
66	93197.5
67	73882.0
68	73882.0
69	59954.5
70	46027.0
71	38155.5
72	30284.0
73	24610.5
74	18937.0
75	18937.0
76	15097.0
77	11257.0
78	9056.5
79	6856.0
80	5237.0
81	3618.0
82	3618.0
83	2837.5
84	2057.0
85	1606.0
86	1155.0
87	840.5
88	526.0
89	526.0
90	357.5
91	189.0
92	122.5
93	56.0
94	44.5
95	33.0
96	33.0
97	22.0
98	11.0
99	8.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4182122.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.29949767536589
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.99869008100406	43.171894936801586
2	9.705721865685776	10.34620200035734
3	3.284843302806939	5.25241493945699
4	1.6037072503608685	3.419071634503062
5	0.9130586761465085	2.4332784393371742
6	0.6369077790439717	2.036811881314597
7	0.4564033251783796	1.7028247578562015
8	0.3350386908814146	1.4285915140633272
9	0.2592278332636197	1.2435041966781994
>10	1.6284852188983518	16.39055078760017
>50	0.12254467774909239	4.462212471210602
>100	0.05313070913994158	4.887858006863906
>500	0.0015236011388269995	0.539979936890815
>1k	5.377415267159217E-4	0.6726341919291671
>5k	0.0	0.0
>10k+	1.7924717557196915E-4	2.0121703051368494
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	41725	0.9976992541107121	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16555	0.39585167529785115	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	15067	0.36027165156827085	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10899	0.26060932703541406	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4794	0.11463080225780119	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	4459	0.10662051465739164	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.391130626987926E-5	2.391130626987926E-5
2	0.0	0.0	0.0	2.391130626987926E-5	2.391130626987926E-5
3	0.0	0.0	0.0	2.391130626987926E-5	2.391130626987926E-5
4	0.0	0.0	0.0	4.782261253975852E-5	2.391130626987926E-5
5	0.0	0.0	0.0	7.173391880963779E-5	2.391130626987926E-5
6	0.0	0.0	0.0	7.173391880963779E-5	7.173391880963779E-5
7	0.0	0.0	0.0	7.173391880963779E-5	7.173391880963779E-5
8	2.391130626987926E-5	0.0	0.0	7.173391880963779E-5	7.173391880963779E-5
9	2.391130626987926E-5	0.0	0.0	7.173391880963779E-5	1.1955653134939632E-4
10	2.391130626987926E-5	0.0	0.0	7.173391880963779E-5	1.1955653134939632E-4
11	2.391130626987926E-5	0.0	0.0	2.1520175642891336E-4	1.1955653134939632E-4
12	2.391130626987926E-5	0.0	0.0	2.8693567523855115E-4	1.1955653134939632E-4
13	2.391130626987926E-5	0.0	0.0	2.8693567523855115E-4	1.4346783761927558E-4
14	4.782261253975852E-5	0.0	0.0	4.064922065879475E-4	2.3911306269879264E-4
15	4.782261253975852E-5	2.391130626987926E-5	0.0	5.260487379373438E-4	2.630243689686719E-4
16	4.782261253975852E-5	2.391130626987926E-5	0.0	9.564522507951705E-4	3.347582877783097E-4
17	4.782261253975852E-5	2.391130626987926E-5	0.0	0.0012194766197638424	3.5866959404818894E-4
18	4.782261253975852E-5	2.391130626987926E-5	0.0	0.0013151218448433594	4.064922065879475E-4
19	4.782261253975852E-5	2.391130626987926E-5	0.0	0.0019607271141300997	4.064922065879475E-4
20	4.782261253975852E-5	2.391130626987926E-5	0.0	0.0031084698150843043	4.064922065879475E-4
21	4.782261253975852E-5	2.391130626987926E-5	0.0	0.004614882110086697	4.3040351285782673E-4
22	4.782261253975852E-5	2.391130626987926E-5	0.0	0.007699440618901122	4.3040351285782673E-4
23	4.782261253975852E-5	2.391130626987926E-5	0.0	0.009253675526443274	6.456052692867401E-4
24	4.782261253975852E-5	2.391130626987926E-5	0.0	0.012290411422717942	6.934278818264986E-4
25	4.782261253975852E-5	2.391130626987926E-5	0.0	0.014514162905816712	6.934278818264986E-4
26	4.782261253975852E-5	2.391130626987926E-5	0.0	0.018076947540028723	6.934278818264986E-4
27	4.782261253975852E-5	2.391130626987926E-5	0.0	0.034121434047117705	7.412504943662572E-4
28	4.782261253975852E-5	2.391130626987926E-5	0.0	0.14645675090301047	7.412504943662572E-4
29	4.782261253975852E-5	2.391130626987926E-5	0.0	0.3472878122637264	7.412504943662572E-4
30	4.782261253975852E-5	2.391130626987926E-5	0.0	0.5912787814415744	7.412504943662572E-4
31	4.782261253975852E-5	2.391130626987926E-5	0.0	1.1797599400495729	7.412504943662572E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	5320	0.0	16.8656	37
AACGCAG	14780	0.0	15.658661	5
TATACAC	2020	0.0	15.386138	37
TAGATCG	125	2.9630828E-6	14.799999	5
GTATAGA	485	0.0	14.494845	1
CGCAATC	230	1.8189894E-12	14.478261	22
TATACGA	105	1.6572804E-4	14.095238	2
ACGCAGA	16730	0.0	13.977286	6
CTAATAC	465	0.0	13.924731	3
CGCAGAG	16900	0.0	13.869527	7
TCGTTAC	445	0.0	13.30337	23
TATACAG	640	0.0	13.296875	5
TAGTACT	505	0.0	13.188118	4
AGAGTAC	17820	0.0	13.153481	10
CGAACCG	155	1.890714E-6	13.129032	18
TAAGAGT	635	0.0	13.110236	4
TAATACG	170	3.7381506E-7	13.058823	4
CAGAGTA	18080	0.0	13.035952	9
AACGGTA	185	7.3983756E-8	13.0	18
TATACTG	675	0.0	12.881481	5
>>END_MODULE