FastQCFastQC Report
Thu 9 Feb 2017
SRR2727389.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727389.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3640046
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT553821.5214642891875543No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT212890.5848552463347991No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT198960.5465864991816037No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151280.41559914352730704No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76420.2099424018267901No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA74630.20502488155369464No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68510.1882119072121616No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50320.13824001125260504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGAT6000.016.6514
CGCGTGA1708.54925E-1116.3235310
CTTATAC41050.016.17904937
TAAGAGT5600.015.8571424
AGTCGGT6350.015.73228411
AACGCAG224600.015.2958585
ACGGATG13500.015.21111128
TCGGTGA6950.014.90647513
CGACGCG2006.184564E-1114.817
CGCAATC2006.184564E-1114.822
CAATACT3750.014.7999994
GTTACGG11000.014.63181825
TACGGAT13950.014.58781427
GTATTAG3300.014.5757571
TATACCG1651.6485501E-814.5757575
TTACGGA14000.014.53571426
TGTTACG6000.014.49166724
CGTATAC1152.2123353E-514.4782613
CCGCTTA2059.276846E-1114.43902525
TCGTTAC5950.014.30252223
Produced by FastQC (version 0.11.5)