##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727389.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3640046
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31495343740161	33.0	31.0	34.0	30.0	34.0
2	32.435701362015756	34.0	31.0	34.0	31.0	34.0
3	32.52630268958139	34.0	31.0	34.0	31.0	34.0
4	36.05366882726207	37.0	35.0	37.0	35.0	37.0
5	35.87084339044067	37.0	35.0	37.0	35.0	37.0
6	35.90057350923587	37.0	35.0	37.0	35.0	37.0
7	35.86022649164324	37.0	35.0	37.0	35.0	37.0
8	35.82241213435215	37.0	35.0	37.0	35.0	37.0
9	37.651327483224115	39.0	37.0	39.0	35.0	39.0
10	37.48138814729264	39.0	37.0	39.0	35.0	39.0
11	37.60024735951139	39.0	37.0	39.0	35.0	39.0
12	37.53319793211404	39.0	37.0	39.0	35.0	39.0
13	37.61669165719334	39.0	37.0	39.0	35.0	39.0
14	38.79419380963867	40.0	38.0	41.0	35.0	41.0
15	38.72268015294312	40.0	38.0	41.0	35.0	41.0
16	38.689462715581065	40.0	38.0	41.0	35.0	41.0
17	38.65552907847868	40.0	38.0	41.0	35.0	41.0
18	38.650408538793194	40.0	38.0	41.0	35.0	41.0
19	38.714792340536356	40.0	38.0	41.0	35.0	41.0
20	38.674789274641036	40.0	38.0	41.0	34.0	41.0
21	38.627507729297925	40.0	38.0	41.0	34.0	41.0
22	38.5771770466637	40.0	38.0	41.0	34.0	41.0
23	38.49829727426521	40.0	38.0	41.0	34.0	41.0
24	38.45633214525311	40.0	38.0	41.0	34.0	41.0
25	38.33079774266589	40.0	38.0	41.0	34.0	41.0
26	38.201170534658075	40.0	38.0	41.0	34.0	41.0
27	38.09261943393023	40.0	37.0	41.0	33.0	41.0
28	37.97311160353468	40.0	37.0	41.0	33.0	41.0
29	37.848313180657605	40.0	37.0	41.0	33.0	41.0
30	37.744154606837384	40.0	37.0	41.0	33.0	41.0
31	37.58920189470133	40.0	36.0	41.0	33.0	41.0
32	37.45000310435638	39.0	36.0	41.0	33.0	41.0
33	37.310289210630856	39.0	36.0	41.0	32.0	41.0
34	37.16188916293915	39.0	36.0	41.0	32.0	41.0
35	37.04064399186164	39.0	35.0	41.0	31.0	41.0
36	36.89291508953458	39.0	35.0	41.0	31.0	41.0
37	36.770264166991296	39.0	35.0	41.0	31.0	41.0
38	36.62926237745347	39.0	35.0	41.0	30.0	41.0
39	36.465485601006144	39.0	35.0	41.0	30.0	41.0
40	36.298691554996836	39.0	35.0	40.0	30.0	41.0
41	36.13244612842805	39.0	35.0	40.0	29.0	41.0
42	35.949920962537284	39.0	35.0	40.0	28.0	41.0
43	34.31409163510571	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	22.0
11	27.0
12	21.0
13	20.0
14	13.0
15	26.0
16	29.0
17	51.0
18	101.0
19	215.0
20	512.0
21	903.0
22	1783.0
23	3365.0
24	5741.0
25	9352.0
26	14433.0
27	22206.0
28	32532.0
29	45116.0
30	58965.0
31	73724.0
32	92616.0
33	119757.0
34	160462.0
35	217597.0
36	321493.0
37	545488.0
38	943726.0
39	969743.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.50945235307466	16.986186438303253	12.752338844069552	24.752022364552538
2	16.348199995274783	22.734740165371537	36.4061608012646	24.51089903808908
3	18.79198779356085	24.0731023728821	30.96416913412633	26.170740699430723
4	13.213486862528661	15.040661574057031	34.817362198170024	36.92848936524429
5	14.38698851607919	38.18718224989465	34.01591628237665	13.409912951649511
6	34.66750145465195	35.76037225903189	15.006403765227141	14.56572252108902
7	28.90100839384997	31.11359032275966	21.830246101285532	18.155155182104842
8	28.337773753408612	35.91089233487709	19.550137553206746	16.20119635850756
9	27.064136002676893	13.62702559253372	19.09327519487391	40.21556320991548
10	14.747231216308803	28.656203795226766	34.18962287839219	22.406942110072237
11	35.931194276116294	21.782581868470892	21.83612514786901	20.450098707543805
12	20.523394484575196	26.195108523353827	31.78990045730191	21.491596534769066
13	31.890448637187554	19.140747122426475	25.29671328329367	23.6720909570923
14	21.186737750017446	19.922770206750133	28.951502261235163	29.93898978199726
15	24.898256780271456	28.71178001596683	23.446791606479696	22.94317159728201
16	22.778750598206727	26.40527070262299	26.07304962629593	24.742929072874354
17	22.909215982435388	26.968175676900785	27.51671270088345	22.60589563978038
18	22.590868357158122	26.099230614118614	28.40804759060737	22.90185343811589
19	23.870577459735397	26.474088514266032	28.365300878065824	21.290033147932746
20	22.712075616626823	26.512549566681297	28.233489357002632	22.541885459689244
21	22.11612160945219	27.23251299571489	28.7275215752768	21.923843819556126
22	22.85770564437922	26.36807337050136	27.727341907217657	23.046879077901764
23	22.011150408538793	26.416369463462825	28.37953146745948	23.192948660538907
24	21.95562913215932	26.28700296644603	28.69491209726471	23.062455804129947
25	22.469908347312096	26.360161382575935	28.208654506014486	22.961275764097486
26	22.134060943185883	26.75672230515768	28.819443490549297	22.28977326110714
27	22.84306297228112	27.233309688943493	28.22615428486343	21.697473053911956
28	22.303097268551003	27.675859041341784	27.88110370033785	22.13993998976936
29	22.14059932209648	27.613140053724592	28.520601113282634	21.725659510896293
30	22.627351412592038	27.40536795414124	28.065991473734126	21.9012891595326
31	22.03491384449537	26.79551302373651	28.663868533529524	22.505704598238594
32	21.58055145456953	27.434241215632987	28.36738876376837	22.61781856602911
33	22.054501509046865	26.650871994474794	28.336207839131706	22.958418657346638
34	22.292822673120067	27.003587317303136	28.795817415494202	21.9077725940826
35	22.151231055871275	27.004493899252925	28.363075631461797	22.481199413414007
36	21.893267282885986	26.813727079273175	28.78702631779928	22.50597932004156
37	21.431432459919463	26.63367440960911	28.887766802946995	23.047126327524435
38	21.84255363805842	26.494610232947608	28.944881465783673	22.7179546632103
39	21.131381306719753	26.51326384336901	29.623883873995	22.73147097591624
40	21.856454561288512	26.245877112541983	29.545643104510216	22.352025221659286
41	21.162754536618493	26.308898294142434	29.642207818252846	22.886139350986223
42	20.748116919401568	26.498346449467945	29.627922284498602	23.125614346631885
43	20.3329848029393	25.95082589615626	29.36363991004509	24.352549390859345
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	648.0
1	941.0
2	1234.0
3	6740.0
4	12246.0
5	12246.0
6	15285.0
7	18324.0
8	16359.0
9	14394.0
10	18182.0
11	21970.0
12	21970.0
13	34412.5
14	46855.0
15	72958.0
16	99061.0
17	78707.0
18	58353.0
19	58353.0
20	60329.5
21	62306.0
22	47577.0
23	32848.0
24	36534.0
25	40220.0
26	40220.0
27	46674.0
28	53128.0
29	60289.0
30	67450.0
31	74315.0
32	81180.0
33	81180.0
34	91648.5
35	102117.0
36	116131.0
37	130145.0
38	145695.5
39	161246.0
40	161246.0
41	178483.5
42	195721.0
43	212607.0
44	229493.0
45	249103.0
46	268713.0
47	268713.0
48	286328.5
49	303944.0
50	311909.0
51	319874.0
52	315277.0
53	310680.0
54	310680.0
55	287578.0
56	264476.0
57	239063.5
58	213651.0
59	192930.5
60	172210.0
61	172210.0
62	148925.5
63	125641.0
64	105865.0
65	86089.0
66	71102.5
67	56116.0
68	56116.0
69	45539.0
70	34962.0
71	28867.5
72	22773.0
73	18131.0
74	13489.0
75	13489.0
76	10845.0
77	8201.0
78	6514.5
79	4828.0
80	3721.0
81	2614.0
82	2614.0
83	2062.5
84	1511.0
85	1164.0
86	817.0
87	573.5
88	330.0
89	330.0
90	224.5
91	119.0
92	82.0
93	45.0
94	32.0
95	19.0
96	19.0
97	11.0
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3640046.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.85001024614512
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.64889041310313	43.96793585331983
2	9.709215352963021	10.456826924781764
3	3.0705955489102688	4.9605480531175665
4	1.5333837287282208	3.3029091801314756
5	0.8821797117961402	2.3752693259581745
6	0.5779046792395329	1.8672103739006431
7	0.4240148331084523	1.5983242175185368
8	0.3089851072858683	1.3311080954600205
9	0.24127692830285372	1.1693488555140383
>10	1.4537123941189898	14.455666550249955
>50	0.09889258294973705	3.6366065733860022
>100	0.04572073015756952	4.4875494330892645
>500	0.0033499737531229854	1.2810857850828896
>1k	0.0014719581596793043	1.3190236328486618
>5k	2.03028711679904E-4	0.7376561358390253
>10k+	2.03028711679904E-4	3.0529310098021316
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	55382	1.5214642891875543	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	21289	0.5848552463347991	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	19896	0.5465864991816037	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15128	0.41559914352730704	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7642	0.2099424018267901	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	7463	0.20502488155369464	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6851	0.1882119072121616	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5032	0.13824001125260504	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.7472180296622627E-5	0.0
3	0.0	0.0	0.0	2.7472180296622627E-5	0.0
4	0.0	0.0	0.0	8.241654088986788E-5	0.0
5	0.0	0.0	0.0	8.241654088986788E-5	0.0
6	0.0	0.0	0.0	8.241654088986788E-5	0.0
7	0.0	0.0	0.0	8.241654088986788E-5	0.0
8	0.0	0.0	0.0	8.241654088986788E-5	0.0
9	0.0	0.0	0.0	1.098887211864905E-4	0.0
10	2.7472180296622627E-5	0.0	0.0	1.6483308177973576E-4	0.0
11	2.7472180296622627E-5	0.0	0.0	2.472496226696036E-4	0.0
12	2.7472180296622627E-5	0.0	0.0	3.8461052415271675E-4	0.0
13	2.7472180296622627E-5	0.0	0.0	4.670270650425846E-4	0.0
14	2.7472180296622627E-5	0.0	0.0	6.043879665256978E-4	5.494436059324525E-5
15	2.7472180296622627E-5	0.0	0.0	9.065819497885466E-4	1.3736090148311312E-4
16	2.7472180296622627E-5	0.0	0.0	0.0014834977360176217	1.9230526207635837E-4
17	2.7472180296622627E-5	0.0	0.0	0.001895580440466961	1.9230526207635837E-4
18	2.7472180296622627E-5	0.0	0.0	0.0024450240463994135	1.9230526207635837E-4
19	5.494436059324525E-5	0.0	0.0	0.0036812721597474316	1.9230526207635837E-4
20	1.3736090148311312E-4	2.7472180296622627E-5	0.0	0.005302130797248166	1.9230526207635837E-4
21	1.6483308177973576E-4	2.7472180296622627E-5	0.0	0.007994404466317185	2.19777442372981E-4
22	1.6483308177973576E-4	2.7472180296622627E-5	0.0	0.012911924739412634	2.472496226696036E-4
23	1.6483308177973576E-4	2.7472180296622627E-5	0.0	0.018433832979033782	2.7472180296622624E-4
24	1.6483308177973576E-4	2.7472180296622627E-5	0.0	0.028955678032640247	2.7472180296622624E-4
25	1.9230526207635837E-4	2.7472180296622627E-5	0.0	0.038378635874381804	2.7472180296622624E-4
26	1.9230526207635837E-4	2.7472180296622627E-5	0.0	0.04777412153582675	3.021939832628489E-4
27	1.9230526207635837E-4	2.7472180296622627E-5	0.0	0.06464204023795303	3.021939832628489E-4
28	1.9230526207635837E-4	2.7472180296622627E-5	0.0	0.15266290590833193	3.021939832628489E-4
29	1.9230526207635837E-4	2.7472180296622627E-5	0.0	0.31928167940734814	3.021939832628489E-4
30	1.9230526207635837E-4	2.7472180296622627E-5	0.0	0.5227955910447285	3.021939832628489E-4
31	1.9230526207635837E-4	2.7472180296622627E-5	0.0	1.0493548707900944	3.571383438560941E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTGAT	600	0.0	16.65	14
CGCGTGA	170	8.54925E-11	16.32353	10
CTTATAC	4105	0.0	16.179049	37
TAAGAGT	560	0.0	15.857142	4
AGTCGGT	635	0.0	15.732284	11
AACGCAG	22460	0.0	15.295858	5
ACGGATG	1350	0.0	15.211111	28
TCGGTGA	695	0.0	14.906475	13
CGACGCG	200	6.184564E-11	14.8	17
CGCAATC	200	6.184564E-11	14.8	22
CAATACT	375	0.0	14.799999	4
GTTACGG	1100	0.0	14.631818	25
TACGGAT	1395	0.0	14.587814	27
GTATTAG	330	0.0	14.575757	1
TATACCG	165	1.6485501E-8	14.575757	5
TTACGGA	1400	0.0	14.535714	26
TGTTACG	600	0.0	14.491667	24
CGTATAC	115	2.2123353E-5	14.478261	3
CCGCTTA	205	9.276846E-11	14.439025	25
TCGTTAC	595	0.0	14.302522	23
>>END_MODULE