FastQCFastQC Report
Thu 9 Feb 2017
SRR2727388.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727388.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2842289
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT271860.9564826096149969No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT104440.36745031909140835No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT96060.3379670399456213No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69740.24536561904859078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTCG1258.592906E-917.765
AACGCAG100550.017.2212835
TACATCG1301.3955287E-817.0769235
CTTATAC33950.016.40206137
ACCGTGA4200.015.4166668
GACCGTG4450.015.3820237
ACGCAGA113050.015.3498466
CGCAGAG114450.015.1944087
GTATTAG3800.015.0921061
CGCGTGA1252.9622752E-614.79999910
CGAACCG908.279299E-414.38888918
GGTATCA58000.014.3853451
AGAGTAC120300.014.31712410
GCAGAGT123900.014.0952398
CAGAGTA122850.014.0952399
GTACTAC2900.014.0344831
TATACTG5000.013.695
GTACCGT2051.4260877E-913.5365856
CAACGCA129550.013.4805094
TCAACGC129850.013.47785953
Produced by FastQC (version 0.11.5)