##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727388.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2842289
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.922326336273336	31.0	31.0	34.0	30.0	34.0
2	32.08480136960035	33.0	31.0	34.0	30.0	34.0
3	32.1590823452506	34.0	31.0	34.0	30.0	34.0
4	35.80964532459578	37.0	35.0	37.0	35.0	37.0
5	35.49780652143396	37.0	35.0	37.0	33.0	37.0
6	35.50403460028168	37.0	35.0	37.0	33.0	37.0
7	35.47989912355851	37.0	35.0	37.0	33.0	37.0
8	35.41715708712238	37.0	35.0	37.0	33.0	37.0
9	37.20710561100578	39.0	37.0	39.0	34.0	39.0
10	36.94862626566123	39.0	37.0	39.0	33.0	39.0
11	37.0878629864873	39.0	37.0	39.0	33.0	39.0
12	36.984714784457175	39.0	37.0	39.0	33.0	39.0
13	37.097135794424844	39.0	37.0	39.0	33.0	39.0
14	38.18688177029148	40.0	38.0	41.0	33.0	41.0
15	38.08108922069501	40.0	37.0	41.0	33.0	41.0
16	38.046835138861674	40.0	37.0	41.0	33.0	41.0
17	37.9745339055951	40.0	37.0	41.0	33.0	41.0
18	37.932275711583166	40.0	37.0	41.0	33.0	41.0
19	37.982879292007254	40.0	37.0	41.0	33.0	41.0
20	37.93241609139676	40.0	37.0	41.0	33.0	41.0
21	37.87821998396363	40.0	37.0	41.0	33.0	41.0
22	37.81968617547336	39.0	37.0	41.0	33.0	41.0
23	37.75310603531168	39.0	37.0	41.0	33.0	41.0
24	37.68453665338043	39.0	37.0	41.0	32.0	41.0
25	37.56867897669801	39.0	37.0	41.0	32.0	41.0
26	37.419777158480365	39.0	37.0	40.0	32.0	41.0
27	37.30540701526129	39.0	36.0	40.0	32.0	41.0
28	37.19419489010442	39.0	36.0	40.0	31.0	41.0
29	37.095802713939364	39.0	36.0	40.0	31.0	41.0
30	37.007739184861215	39.0	36.0	40.0	31.0	41.0
31	36.89608023673877	39.0	36.0	40.0	31.0	41.0
32	36.78291475638121	39.0	35.0	40.0	30.0	41.0
33	36.674299481861276	39.0	35.0	40.0	30.0	41.0
34	36.55326675084764	39.0	35.0	40.0	30.0	41.0
35	36.471274033006495	38.0	35.0	40.0	30.0	41.0
36	36.37523946368578	38.0	35.0	40.0	30.0	41.0
37	36.27760723839131	38.0	35.0	40.0	30.0	41.0
38	36.159286054303415	38.0	35.0	40.0	30.0	41.0
39	36.029794296076155	38.0	35.0	40.0	29.0	41.0
40	35.89729369532795	38.0	35.0	40.0	29.0	41.0
41	35.76858651600875	38.0	35.0	40.0	28.0	41.0
42	35.64188722540178	38.0	35.0	40.0	27.0	41.0
43	33.941185431882545	37.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	2.0
10	11.0
11	10.0
12	7.0
13	6.0
14	10.0
15	15.0
16	33.0
17	68.0
18	141.0
19	288.0
20	622.0
21	1189.0
22	2302.0
23	4050.0
24	6505.0
25	10485.0
26	15234.0
27	22170.0
28	31385.0
29	42829.0
30	56673.0
31	72214.0
32	92713.0
33	119300.0
34	158711.0
35	217300.0
36	318374.0
37	501801.0
38	767492.0
39	400345.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.96720002786486	18.636985894115625	13.122346109069133	25.273467968950374
2	17.046120222116752	21.772697990950252	36.437181440733156	24.74400034619984
3	18.38022101200828	22.819284034804344	31.67506893211774	27.125426021069636
4	13.304488037634457	15.82879151275609	36.02367669156796	34.84304375804149
5	13.85724674725195	37.89899619637553	34.853880094529444	13.389876961843077
6	33.86657725516301	37.36404003955967	15.059693085397017	13.709689619880313
7	28.78961287891555	31.692941850740723	21.350678977401667	18.166766292942064
8	27.509764137285124	36.14653541564563	19.326817223723555	17.01688322334569
9	26.4880172283677	13.475160337319675	19.024877484309304	41.011944950003326
10	14.674088384397225	28.37902831133639	34.85532259386713	22.091560710399257
11	36.544383769560376	21.358384034839524	22.354201138589357	19.74303105701074
12	20.783389725675327	26.058785718130707	31.687734779960795	21.47008977623317
13	31.48244249617122	19.58467277606183	25.062124224524666	23.87076050324228
14	21.12853407939868	20.10393735471657	28.18020968311104	30.587318882773708
15	24.412858790925203	28.83436554129436	23.011101263805333	23.741674403975104
16	23.405818338669995	26.821375307014872	25.371557923912736	24.401248430402397
17	23.399555780569813	27.00115998056496	26.811207445829755	22.788076793035472
18	23.383125361284517	26.074442113381153	27.33082385359124	23.211608671743093
19	24.366346983012637	26.0747939424879	27.53896595314551	22.019893121353952
20	23.740724465386876	26.103855026705585	27.339689947081386	22.81573056082615
21	23.45697429079168	26.25049739839967	27.47690329871452	22.815625012094127
22	23.7833309702145	25.921326086122843	27.18615172489497	23.10919121876769
23	23.157356623481988	25.95320180319454	27.29761118591389	23.591830387409583
24	23.16354881576082	26.081302780962805	27.46951488747274	23.285633515803635
25	23.53163242724438	25.63564085144051	27.433522769852047	23.39920395146306
26	23.151269979935186	26.041229445703795	27.63906133401635	23.168439240344664
27	23.50345091579357	26.400587695339915	27.1552259464115	22.940735442455008
28	23.15035522425763	26.365439967575426	27.323259527796083	23.160945280370857
29	23.078019159909495	26.666535317133476	27.328290684022633	22.927154838934392
30	23.085724217347355	26.642470206231668	27.264679981521933	23.00712559489904
31	23.203235139002405	25.92255748799647	27.37251560274131	23.501691770259818
32	22.612901080783836	26.42268256324392	27.472012874130673	23.492403481841574
33	23.027637231822663	25.829709786724713	27.5149008422437	23.627752139208926
34	23.104934086575994	25.800824617060403	28.066252235434185	23.027989060929414
35	23.010538337234532	25.897401706863725	27.782466877928318	23.309593077973425
36	22.780477284329635	25.627091404146444	28.092604235529883	23.49982707599403
37	22.63284979113665	25.26400376597876	28.01604622190073	24.087100220983864
38	22.340233523051314	25.101036523731402	28.627665941077773	23.93106401213951
39	22.311066890101607	24.929449468368627	29.003736073284596	23.755747568245173
40	22.293158788567947	24.932651113240066	29.166632949710603	23.60755714848138
41	21.869873190235054	24.710506215237086	29.628408652322126	23.791211942205738
42	21.419637482324987	25.018004854538017	29.428569719687196	24.133787943449807
43	21.140285171564184	24.3906231913785	29.49450249429245	24.974589142764863
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1143.0
1	1084.0
2	1025.0
3	2870.5
4	4716.0
5	4716.0
6	5849.0
7	6982.0
8	6431.5
9	5881.0
10	7866.0
11	9851.0
12	9851.0
13	15315.5
14	20780.0
15	33591.5
16	46403.0
17	36632.0
18	26861.0
19	26861.0
20	29247.5
21	31634.0
22	27304.5
23	22975.0
24	27390.0
25	31805.0
26	31805.0
27	37997.0
28	44189.0
29	50210.0
30	56231.0
31	64263.5
32	72296.0
33	72296.0
34	82212.5
35	92129.0
36	104108.0
37	116087.0
38	129663.5
39	143240.0
40	143240.0
41	157200.0
42	171160.0
43	184441.5
44	197723.0
45	208653.0
46	219583.0
47	219583.0
48	230543.0
49	241503.0
50	244041.5
51	246580.0
52	238937.0
53	231294.0
54	231294.0
55	218200.0
56	205106.0
57	188736.0
58	172366.0
59	155640.0
60	138914.0
61	138914.0
62	120237.5
63	101561.0
64	85283.5
65	69006.0
66	56498.5
67	43991.0
68	43991.0
69	35529.0
70	27067.0
71	21917.0
72	16767.0
73	13591.0
74	10415.0
75	10415.0
76	8324.0
77	6233.0
78	5064.0
79	3895.0
80	3034.5
81	2174.0
82	2174.0
83	1766.0
84	1358.0
85	1056.0
86	754.0
87	573.5
88	393.0
89	393.0
90	269.5
91	146.0
92	92.5
93	39.0
94	30.5
95	22.0
96	22.0
97	15.5
98	9.0
99	5.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2842289.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.259964654831556
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.55889012822864	52.02373545980469
2	8.97283798344314	11.172971514054002
3	2.7877694072027275	5.206992742747876
4	1.3554590065847676	3.375633193641629
5	0.7839327689842462	2.4403813244361703
6	0.5134053131461359	1.917875799004869
7	0.3595292198711762	1.5668993565091014
8	0.2619809316793325	1.3048738837276066
9	0.20503639007807944	1.1489002559293935
>10	1.1167753644693144	12.491038125735363
>50	0.06112155220632969	2.5506051349771504
>100	0.02207665541066078	2.2453312674904335
>500	6.773021117178999E-4	0.27100485689452664
>1k	2.8220921311946533E-4	0.3787812955996203
>5k	1.1288368524778612E-4	0.5826323296262831
>10k+	1.1288368524778612E-4	1.3223434598212926
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	27186	0.9564826096149969	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10444	0.36745031909140835	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9606	0.3379670399456213	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6974	0.24536561904859078	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.518291067516357E-5
2	0.0	0.0	0.0	0.0	3.518291067516357E-5
3	0.0	0.0	0.0	0.0	3.518291067516357E-5
4	0.0	0.0	0.0	0.0	3.518291067516357E-5
5	0.0	0.0	0.0	0.0	3.518291067516357E-5
6	1.0554873202549072E-4	0.0	0.0	3.518291067516357E-5	7.036582135032714E-5
7	1.0554873202549072E-4	0.0	0.0	3.518291067516357E-5	7.036582135032714E-5
8	1.0554873202549072E-4	0.0	0.0	3.518291067516357E-5	7.036582135032714E-5
9	1.0554873202549072E-4	0.0	0.0	1.0554873202549072E-4	7.036582135032714E-5
10	1.0554873202549072E-4	0.0	0.0	1.4073164270065428E-4	7.036582135032714E-5
11	1.4073164270065428E-4	0.0	0.0	3.5182910675163575E-4	7.036582135032714E-5
12	1.4073164270065428E-4	0.0	0.0	4.9256074945229E-4	7.036582135032714E-5
13	1.4073164270065428E-4	0.0	0.0	4.9256074945229E-4	7.036582135032714E-5
14	1.4073164270065428E-4	0.0	0.0	5.277436601274536E-4	1.4073164270065428E-4
15	1.4073164270065428E-4	0.0	0.0	7.036582135032715E-4	1.4073164270065428E-4
16	1.4073164270065428E-4	0.0	0.0	8.443898562039258E-4	2.1109746405098144E-4
17	1.7591455337581788E-4	0.0	0.0	0.0013721335163313793	2.1109746405098144E-4
18	1.7591455337581788E-4	0.0	0.0	0.0014424993376817065	2.1109746405098144E-4
19	1.7591455337581788E-4	0.0	0.0	0.0016887797124078515	2.1109746405098144E-4
20	2.46280374726145E-4	0.0	0.0	0.002146157551184978	2.1109746405098144E-4
21	2.46280374726145E-4	0.0	0.0	0.003377559424815703	2.1109746405098144E-4
22	2.46280374726145E-4	0.0	0.0	0.0053478024226248635	2.1109746405098144E-4
23	2.46280374726145E-4	0.0	0.0	0.00679030176030657	3.166461960764722E-4
24	2.46280374726145E-4	0.0	0.0	0.008901276400816385	3.166461960764722E-4
25	2.46280374726145E-4	0.0	0.0	0.0098512149890458	3.166461960764722E-4
26	2.8146328540130857E-4	0.0	0.0	0.011469628880103324	3.166461960764722E-4
27	2.8146328540130857E-4	0.0	0.0	0.024557671651264174	3.166461960764722E-4
28	2.8146328540130857E-4	0.0	0.0	0.12686957589463985	3.166461960764722E-4
29	2.8146328540130857E-4	0.0	0.0	0.3004972400765721	3.166461960764722E-4
30	2.8146328540130857E-4	0.0	0.0	0.5073375719358587	3.166461960764722E-4
31	2.8146328540130857E-4	0.0	0.0	1.0711788984160302	3.166461960764722E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTCG	125	8.592906E-9	17.76	5
AACGCAG	10055	0.0	17.221283	5
TACATCG	130	1.3955287E-8	17.076923	5
CTTATAC	3395	0.0	16.402061	37
ACCGTGA	420	0.0	15.416666	8
GACCGTG	445	0.0	15.382023	7
ACGCAGA	11305	0.0	15.349846	6
CGCAGAG	11445	0.0	15.194408	7
GTATTAG	380	0.0	15.092106	1
CGCGTGA	125	2.9622752E-6	14.799999	10
CGAACCG	90	8.279299E-4	14.388889	18
GGTATCA	5800	0.0	14.385345	1
AGAGTAC	12030	0.0	14.317124	10
GCAGAGT	12390	0.0	14.095239	8
CAGAGTA	12285	0.0	14.095239	9
GTACTAC	290	0.0	14.034483	1
TATACTG	500	0.0	13.69	5
GTACCGT	205	1.4260877E-9	13.536585	6
CAACGCA	12955	0.0	13.480509	4
TCAACGC	12985	0.0	13.4778595	3
>>END_MODULE