FastQCFastQC Report
Thu 9 Feb 2017
SRR2727387.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727387.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3900399
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT244970.6280639493549249No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT103890.2663573649772754No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT93100.23869352853387563No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62560.16039384688592115No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGG752.0688651E-417.26666629
AACGCAG94150.017.2326075
CTTATAC28150.017.02131337
CGCTACG1708.54925E-1116.3235310
TAATACC3750.016.284
ACGCAGA107000.015.2495336
GGTATCA54150.015.203141
CTATACG1353.9789302E-715.0740754
CGCAGAG110000.014.8336377
CGCAATC1752.242814E-914.822
ACGCGCA2251.8189894E-1214.819
TAAGACT6600.014.5757574
AACGACG1956.7484507E-1014.2307715
AGAGTAC114550.014.21213410
CAGAGTA117250.014.0268669
TCTTATA43800.013.93835737
CCCCTAT4650.013.9247311
TCGTTAC3750.013.81333423
GCAGAGT120700.013.7332228
TCAACGC119250.013.6675053
Produced by FastQC (version 0.11.5)