##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727387.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3900399
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33704244104257	33.0	31.0	34.0	31.0	34.0
2	32.48673225482829	34.0	31.0	34.0	31.0	34.0
3	32.58016218340739	34.0	31.0	34.0	31.0	34.0
4	36.11487747791956	37.0	35.0	37.0	35.0	37.0
5	35.923640889047505	37.0	35.0	37.0	35.0	37.0
6	35.93529123558898	37.0	35.0	37.0	35.0	37.0
7	35.90414288384342	37.0	35.0	37.0	35.0	37.0
8	35.85625829562565	37.0	35.0	37.0	35.0	37.0
9	37.69348392305505	39.0	37.0	39.0	35.0	39.0
10	37.514607864477455	39.0	37.0	39.0	35.0	39.0
11	37.629644044109334	39.0	37.0	39.0	35.0	39.0
12	37.5468204663164	39.0	37.0	39.0	35.0	39.0
13	37.62862132822821	39.0	37.0	39.0	35.0	39.0
14	38.87132213909398	40.0	38.0	41.0	36.0	41.0
15	38.79849369256838	40.0	38.0	41.0	35.0	41.0
16	38.77144005010769	40.0	38.0	41.0	35.0	41.0
17	38.709567918564225	40.0	38.0	41.0	35.0	41.0
18	38.672255838441146	40.0	38.0	41.0	35.0	41.0
19	38.72410309817021	40.0	38.0	41.0	35.0	41.0
20	38.68692946542136	40.0	38.0	41.0	34.0	41.0
21	38.649211785768586	40.0	38.0	41.0	34.0	41.0
22	38.612098403265925	40.0	38.0	41.0	34.0	41.0
23	38.55459069700305	40.0	38.0	41.0	34.0	41.0
24	38.50956402152703	40.0	38.0	41.0	34.0	41.0
25	38.4036581898416	40.0	38.0	41.0	34.0	41.0
26	38.30506904550022	40.0	38.0	41.0	34.0	41.0
27	38.2201538868203	40.0	38.0	41.0	34.0	41.0
28	38.13488466179999	40.0	38.0	41.0	34.0	41.0
29	38.068895002793305	40.0	38.0	41.0	33.0	41.0
30	38.01374423488469	40.0	37.0	41.0	33.0	41.0
31	37.927713292922085	40.0	37.0	41.0	33.0	41.0
32	37.843297826709524	40.0	37.0	41.0	33.0	41.0
33	37.75622237622356	40.0	37.0	41.0	33.0	41.0
34	37.67636746907176	40.0	37.0	41.0	33.0	41.0
35	37.616426422014776	40.0	37.0	41.0	33.0	41.0
36	37.54405510820816	40.0	37.0	41.0	33.0	41.0
37	37.468343366922205	40.0	37.0	41.0	32.0	41.0
38	37.38473320293642	40.0	37.0	41.0	32.0	41.0
39	37.288903776254685	40.0	37.0	41.0	32.0	41.0
40	37.18466033859613	39.0	36.0	41.0	31.0	41.0
41	37.09060611491286	39.0	36.0	41.0	31.0	41.0
42	36.987702540176016	39.0	36.0	41.0	31.0	41.0
43	35.34224652400947	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	11.0
11	14.0
12	11.0
13	10.0
14	6.0
15	13.0
16	18.0
17	42.0
18	81.0
19	162.0
20	353.0
21	752.0
22	1486.0
23	2821.0
24	4771.0
25	7574.0
26	12076.0
27	18067.0
28	26599.0
29	36853.0
30	50655.0
31	66658.0
32	87931.0
33	116020.0
34	157012.0
35	217752.0
36	321438.0
37	537567.0
38	1070791.0
39	1162851.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.67609570200382	17.937857126924705	12.17480570577523	26.211241465296244
2	16.847225117225186	20.3992206951135	37.08669292551864	25.66686126214267
3	17.966982352318315	22.08830429912427	31.29082434899609	28.653888999561328
4	13.0756109823636	15.077970228174092	36.12304792407136	35.723370865390955
5	13.816586456924023	37.491446387920824	35.26595612397603	13.426011031179119
6	34.850742193298686	36.88186772686589	14.88101601913035	13.386374060705073
7	29.76890313016694	31.432963653205736	20.97998179160645	17.818151425020876
8	27.424783977228994	36.530031927502804	18.953035317668782	17.09214877759942
9	26.856713890040478	13.305125962754067	18.538872561499478	41.299287585705976
10	14.518335175452563	27.91019585432157	35.00413675626519	22.567332213960675
11	37.23877992995075	20.90063093545045	22.053154049111388	19.807435085487406
12	20.85291786814631	25.216830380686694	31.709166164794933	22.221085586372062
13	31.395839246189944	19.23480136262982	24.86350755397076	24.505851837209473
14	21.393119011670343	19.381094088066376	27.356329442192965	31.869457458070315
15	24.814640758548034	28.36512367068087	22.42455194968515	24.395683621085944
16	24.239084257790037	26.561641514111763	24.63776141876767	24.561512809330534
17	23.80984612087122	26.708447007601016	26.050616872786602	23.431089998741154
18	23.80661568213919	25.999827197166237	26.5839469244044	23.609610196290173
19	24.795411956571623	25.741725397837506	26.644171532194527	22.818691113396348
20	24.202062404384783	25.6459916023976	26.563692586322578	23.58825340689504
21	23.89091475000378	25.75831344434249	26.797719925576846	23.553051880076882
22	24.254800598605424	25.297104219337562	26.4007092607705	24.047385921286516
23	23.67483429259417	25.370942818926988	26.64491504587095	24.309307842607897
24	23.650067595648547	25.498852809674087	26.748955683764663	24.102123910912702
25	24.104328813539333	25.23734110279487	26.523568486198464	24.134761597467335
26	23.733546234628815	25.549565570086546	26.793053736297235	23.9238344589874
27	24.07640859306958	25.912041306543255	26.316743492140166	23.694806608247003
28	23.793488819989957	25.920399425802337	26.421809666139286	23.864302088068428
29	23.762184330372353	26.19473033399916	26.449422225777415	23.593663109851068
30	24.039668762093314	26.050950172020865	26.235674863007606	23.67370620287822
31	23.971829548720528	25.32043516573561	26.60566265143643	24.102072634107433
32	23.338637918838558	26.027593587220178	26.57348645612923	24.060282037812026
33	23.629505596735104	25.413476928898817	26.676424642709627	24.280592831656453
34	23.88317195240795	25.38417223468676	27.137274930077666	23.59538088282763
35	23.77556757654794	25.565179357291395	26.752570698536225	23.90668236762444
36	23.596842271777835	25.268594315607196	27.097663598006257	24.036899814608713
37	23.53746373127467	24.956344209912885	27.054642358384363	24.451549700428085
38	23.32610073994994	24.930013570406516	27.395992051069644	24.3478936385739
39	23.186832936835437	24.71583035479191	27.84468973558859	24.252646972784067
40	23.366224840074054	24.607892679697642	28.018312998234286	24.00756948199402
41	22.852585081680104	24.471957868925717	28.39468474891928	24.2807723004749
42	22.46377870571703	24.673860289678057	28.386608652089183	24.47575235251573
43	22.268670461663024	24.068896541097462	28.379840113793485	25.282592883446025
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	640.0
1	683.5
2	727.0
3	2471.0
4	4215.0
5	4215.0
6	5184.5
7	6154.0
8	5486.5
9	4819.0
10	6545.5
11	8272.0
12	8272.0
13	12893.0
14	17514.0
15	29045.0
16	40576.0
17	32811.0
18	25046.0
19	25046.0
20	28468.5
21	31891.0
22	28963.0
23	26035.0
24	31850.0
25	37665.0
26	37665.0
27	46118.0
28	54571.0
29	62842.5
30	71114.0
31	81972.5
32	92831.0
33	92831.0
34	107067.5
35	121304.0
36	138470.5
37	155637.0
38	175419.5
39	195202.0
40	195202.0
41	213920.5
42	232639.0
43	251652.5
44	270666.0
45	288542.5
46	306419.0
47	306419.0
48	322294.5
49	338170.0
50	341939.5
51	345709.0
52	338610.0
53	331511.0
54	331511.0
55	314855.5
56	298200.0
57	276482.5
58	254765.0
59	230432.0
60	206099.0
61	206099.0
62	178554.5
63	151010.0
64	126225.0
65	101440.0
66	84256.5
67	67073.0
68	67073.0
69	53874.0
70	40675.0
71	33199.5
72	25724.0
73	20607.0
74	15490.0
75	15490.0
76	12364.0
77	9238.0
78	7270.0
79	5302.0
80	4024.5
81	2747.0
82	2747.0
83	2262.0
84	1777.0
85	1350.0
86	923.0
87	665.5
88	408.0
89	408.0
90	269.0
91	130.0
92	80.5
93	31.0
94	32.0
95	33.0
96	33.0
97	19.5
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3900399.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.57495856768961
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.9801158409223	45.00466582665962
2	9.857917004699383	10.957066581997822
3	3.2497175152842153	5.418087488058465
4	1.6235458685652366	3.609139775130267
5	0.9441448151238515	2.623540449120351
6	0.6028548856005564	2.010218117374811
7	0.4412325756873554	1.7165037478777827
8	0.33476356316912553	1.4883576922477038
9	0.26017760880025637	1.3013423846383323
>10	1.5537405166068263	16.19357557683685
>50	0.1094488806935518	4.138328402761796
>100	0.041463962328643855	3.8446344177027227
>500	5.077151577674495E-4	0.17458162524394452
>1k	1.8462368045582176E-4	0.2258069280232661
>5k	9.231184022791088E-5	0.3992855437476042
>10k+	9.231184022791088E-5	0.894865442578628
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	24497	0.6280639493549249	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10389	0.2663573649772754	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9310	0.23869352853387563	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6256	0.16039384688592115	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.1276805270435154E-5
2	0.0	0.0	0.0	0.0	5.1276805270435154E-5
3	0.0	0.0	0.0	0.0	5.1276805270435154E-5
4	0.0	0.0	0.0	0.0	5.1276805270435154E-5
5	0.0	0.0	0.0	0.0	5.1276805270435154E-5
6	0.0	0.0	0.0	0.0	5.1276805270435154E-5
7	0.0	0.0	0.0	0.0	5.1276805270435154E-5
8	0.0	0.0	0.0	0.0	5.1276805270435154E-5
9	0.0	0.0	0.0	0.0	1.0255361054087031E-4
10	0.0	0.0	0.0	0.0	1.0255361054087031E-4
11	2.5638402635217577E-5	0.0	0.0	2.5638402635217577E-5	1.0255361054087031E-4
12	2.5638402635217577E-5	0.0	0.0	7.691520790565273E-5	1.0255361054087031E-4
13	2.5638402635217577E-5	0.0	0.0	1.0255361054087031E-4	1.0255361054087031E-4
14	2.5638402635217577E-5	0.0	0.0	1.281920131760879E-4	1.281920131760879E-4
15	2.5638402635217577E-5	0.0	0.0	2.3074562371695818E-4	1.281920131760879E-4
16	2.5638402635217577E-5	0.0	0.0	3.332992342578285E-4	1.7946881844652305E-4
17	2.5638402635217577E-5	0.0	0.0	5.127680527043516E-4	2.0510722108174062E-4
18	2.5638402635217577E-5	0.0	0.0	5.384064553395691E-4	2.0510722108174062E-4
19	2.5638402635217577E-5	0.0	0.0	6.66598468515657E-4	2.0510722108174062E-4
20	2.5638402635217577E-5	2.5638402635217577E-5	0.0	0.001025536105408703	2.0510722108174062E-4
21	2.5638402635217577E-5	2.5638402635217577E-5	0.0	0.00169213457392436	2.0510722108174062E-4
22	2.5638402635217577E-5	2.5638402635217577E-5	0.0	0.0028715010951443688	2.0510722108174062E-4
23	2.5638402635217577E-5	2.5638402635217577E-5	0.0	0.003435545953119155	4.358528447986988E-4
24	2.5638402635217577E-5	2.5638402635217577E-5	0.0	0.004486720461163076	4.358528447986988E-4
25	2.5638402635217577E-5	2.5638402635217577E-5	0.0	0.005358426150760474	4.6149124743391636E-4
26	2.5638402635217577E-5	2.5638402635217577E-5	0.0	0.006050663021911348	4.6149124743391636E-4
27	2.5638402635217577E-5	2.5638402635217577E-5	0.0	0.01248590208335096	4.6149124743391636E-4
28	2.5638402635217577E-5	2.5638402635217577E-5	0.0	0.05945545571106956	4.6149124743391636E-4
29	2.5638402635217577E-5	2.5638402635217577E-5	0.0	0.14624144863128105	4.8712965006913393E-4
30	2.5638402635217577E-5	2.5638402635217577E-5	0.0	0.25330741803594964	4.8712965006913393E-4
31	2.5638402635217577E-5	2.5638402635217577E-5	0.0	0.6214236030724036	4.8712965006913393E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGG	75	2.0688651E-4	17.266666	29
AACGCAG	9415	0.0	17.232607	5
CTTATAC	2815	0.0	17.021313	37
CGCTACG	170	8.54925E-11	16.32353	10
TAATACC	375	0.0	16.28	4
ACGCAGA	10700	0.0	15.249533	6
GGTATCA	5415	0.0	15.20314	1
CTATACG	135	3.9789302E-7	15.074075	4
CGCAGAG	11000	0.0	14.833637	7
CGCAATC	175	2.242814E-9	14.8	22
ACGCGCA	225	1.8189894E-12	14.8	19
TAAGACT	660	0.0	14.575757	4
AACGACG	195	6.7484507E-10	14.23077	15
AGAGTAC	11455	0.0	14.212134	10
CAGAGTA	11725	0.0	14.026866	9
TCTTATA	4380	0.0	13.938357	37
CCCCTAT	465	0.0	13.924731	1
TCGTTAC	375	0.0	13.813334	23
GCAGAGT	12070	0.0	13.733222	8
TCAACGC	11925	0.0	13.667505	3
>>END_MODULE