FastQCFastQC Report
Thu 9 Feb 2017
SRR2727386.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727386.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2052141
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT108830.5303241833772631No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT46240.22532564770159558No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41590.20266638598419895No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28400.13839205005893845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT2050.017.1463436
TAGATCG1107.8149606E-716.8181825
CGTATAC700.002593424215.8571423
CTTATAC13000.015.79615537
GGGTATA2600.015.6538461
GTCTTAG3700.015.01
AACGCAG45800.014.94541555
GTATTAG3100.014.9193541
CTATCGG750.004105867414.81
CGTCGAA1001.0940284E-414.822
TATACTG4300.014.6279075
CGCCTTA1651.6470949E-814.57575725
CGGTGAT2059.276846E-1114.43902514
GGTATCA32700.014.4266051
TAATCGA908.27792E-414.38888820
TTAGTAC1551.2114833E-714.322583
CCCTATA2600.014.2307692
TTATACT4050.014.1604944
ACTATAC2750.014.1272743
TAGACGG800.00630084313.8750015
Produced by FastQC (version 0.11.5)