##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727386.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2052141
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42255527276147	34.0	31.0	34.0	31.0	34.0
2	32.568510643274514	34.0	31.0	34.0	31.0	34.0
3	32.65923930178287	34.0	31.0	34.0	31.0	34.0
4	36.1720963617997	37.0	35.0	37.0	35.0	37.0
5	35.98659643757422	37.0	35.0	37.0	35.0	37.0
6	35.997988442314636	37.0	35.0	37.0	35.0	37.0
7	35.96603888329311	37.0	35.0	37.0	35.0	37.0
8	35.917820461654436	37.0	35.0	37.0	35.0	37.0
9	37.75998335397032	39.0	37.0	39.0	35.0	39.0
10	37.59044188484125	39.0	37.0	39.0	35.0	39.0
11	37.693678455817604	39.0	37.0	39.0	35.0	39.0
12	37.61886975602554	39.0	37.0	39.0	35.0	39.0
13	37.69333101380461	39.0	37.0	39.0	35.0	39.0
14	38.97407634270745	40.0	38.0	41.0	36.0	41.0
15	38.89838709913208	40.0	38.0	41.0	36.0	41.0
16	38.87174468031193	40.0	38.0	41.0	36.0	41.0
17	38.80670772622349	40.0	38.0	41.0	35.0	41.0
18	38.77143091044913	40.0	38.0	41.0	35.0	41.0
19	38.81647703544737	40.0	38.0	41.0	35.0	41.0
20	38.78052580207695	40.0	38.0	41.0	35.0	41.0
21	38.7410816313304	40.0	38.0	41.0	35.0	41.0
22	38.70718873605664	40.0	38.0	41.0	35.0	41.0
23	38.64845300590944	40.0	38.0	41.0	34.0	41.0
24	38.60530343675215	40.0	38.0	41.0	34.0	41.0
25	38.499210336911545	40.0	38.0	41.0	34.0	41.0
26	38.40406921356768	40.0	38.0	41.0	34.0	41.0
27	38.33166629388526	40.0	38.0	41.0	34.0	41.0
28	38.247362145193726	40.0	38.0	41.0	34.0	41.0
29	38.18021568693379	40.0	38.0	41.0	34.0	41.0
30	38.125974287341855	40.0	38.0	41.0	34.0	41.0
31	38.04397017553862	40.0	38.0	41.0	33.0	41.0
32	37.96257031071452	40.0	37.0	41.0	33.0	41.0
33	37.87715366536705	40.0	37.0	41.0	33.0	41.0
34	37.80112623840175	40.0	37.0	41.0	33.0	41.0
35	37.75023061280877	40.0	37.0	41.0	33.0	41.0
36	37.67501404630578	40.0	37.0	41.0	33.0	41.0
37	37.600640501797876	40.0	37.0	41.0	33.0	41.0
38	37.51360505930148	40.0	37.0	41.0	32.0	41.0
39	37.42090236489598	40.0	37.0	41.0	32.0	41.0
40	37.31616589698271	40.0	37.0	41.0	32.0	41.0
41	37.22860661133908	40.0	36.0	41.0	31.0	41.0
42	37.13034825579724	39.0	36.0	41.0	31.0	41.0
43	35.47951724564735	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	4.0
12	0.0
13	2.0
14	3.0
15	2.0
16	12.0
17	25.0
18	38.0
19	73.0
20	171.0
21	344.0
22	713.0
23	1290.0
24	2173.0
25	3606.0
26	5673.0
27	8521.0
28	12538.0
29	17839.0
30	24307.0
31	32610.0
32	42982.0
33	56985.0
34	78377.0
35	110509.0
36	164701.0
37	280410.0
38	570379.0
39	637851.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.19316216575761	17.535734630320235	11.898646340577962	26.372456863344183
2	16.779938610456107	20.02440378122166	37.348944346416744	25.846713261905492
3	18.18549505126597	22.142679279835058	30.839693763732605	28.832131905166356
4	13.071908801588194	14.858140839250325	36.063944923862444	36.00600543529904
5	13.884279881353182	37.38670978261241	35.40975010976341	13.319260226271002
6	35.3292000890777	36.740945188464146	14.68588172060302	13.243973001855135
7	30.075175146347156	31.082172228906295	20.989249764026933	17.85340286071961
8	27.487877294981196	36.89215312203206	18.653152975355983	16.96681660763076
9	26.97036899511291	13.311804598222052	18.17175330545026	41.54607310121478
10	14.459971317760328	27.83346758336781	34.99930073031044	22.707260368561418
11	37.666612576816114	20.755737544350023	21.876469501851968	19.701180376981895
12	20.951094491070545	24.827826158144102	31.856729142880535	22.364350207904817
13	31.456220600826164	19.125440210979654	24.798490941899217	24.619848246294968
14	21.503103344263383	19.090890928060013	26.99356428237631	32.4124414453003
15	24.855358379370617	28.265942739801993	22.231951898042094	24.646746982785295
16	24.54495085863983	26.44818265411587	24.339701804115798	24.667164683128497
17	24.067693204316857	26.59646681197832	25.832435490543777	23.503404493161046
18	24.058824418010264	26.037148519521807	26.228558369039945	23.675468693427987
19	24.980203601994212	25.76284962875358	26.163942925949048	23.093003843303165
20	24.36630816303558	25.587618004805712	26.14557186859967	23.900501963559034
21	24.075051373175622	25.65496230522172	26.36816865897616	23.901817662626495
22	24.46444956754921	25.182967447168593	26.055373388085908	24.297209597196296
23	23.91404879099438	25.247826538234946	26.429811596766502	24.408313074004173
24	23.97666632068654	25.34455478449093	26.33006211561486	24.348716779207667
25	24.256715303675527	25.18686581477588	26.240692038217645	24.31572684333094
26	23.9128305511171	25.47553993609601	26.434489637895254	24.177139874891637
27	24.322207879478068	25.759243638716832	25.99675168519122	23.92179679661388
28	24.146391500389104	25.794036569611933	26.109560697827295	23.95001123217167
29	24.079290847948556	25.966685525019965	26.11886805048971	23.835155576541766
30	24.224212663749714	25.887353744211534	25.94251564585474	23.945917946184007
31	24.153311102892054	25.284763571314055	26.32791801343085	24.23400731236304
32	23.733164534015938	25.868544120506336	26.254092676867717	24.144198668610002
33	23.822680800198427	25.378762960244934	26.330451952375594	24.46810428718105
34	24.07363821491798	25.45872822578955	26.608405562775655	23.859227996516807
35	24.034751998035222	25.555943767996446	26.26822425944416	24.141079974524168
36	23.872384987191424	25.342800519067648	26.56279466177032	24.22201983197061
37	23.940850068294527	24.999061955294493	26.52980472589359	24.53028325051739
38	23.714744747071475	25.091940563538273	26.83027140922578	24.363043280164472
39	23.470755664449957	24.990388087368267	27.18507159108463	24.35378465709715
40	23.785353930358585	24.751174505065684	27.32853151903305	24.13494004554268
41	23.274472855422704	24.73270598852613	27.51882058786409	24.47400056818708
42	23.014597924801464	24.85993896130919	27.542649359863674	24.582813754025672
43	22.92079345425095	24.378100725047645	27.57651642845204	25.124589392249362
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	144.0
1	197.0
2	250.0
3	1037.5
4	1825.0
5	1825.0
6	2296.0
7	2767.0
8	2511.0
9	2255.0
10	3026.0
11	3797.0
12	3797.0
13	5986.5
14	8176.0
15	13316.5
16	18457.0
17	15048.5
18	11640.0
19	11640.0
20	13412.0
21	15184.0
22	13778.0
23	12372.0
24	15438.5
25	18505.0
26	18505.0
27	22914.5
28	27324.0
29	31815.5
30	36307.0
31	41921.0
32	47535.0
33	47535.0
34	55028.0
35	62521.0
36	71602.0
37	80683.0
38	90947.0
39	101211.0
40	101211.0
41	111245.0
42	121279.0
43	130635.5
44	139992.0
45	150906.5
46	161821.0
47	161821.0
48	170133.0
49	178445.0
50	180713.0
51	182981.0
52	178910.5
53	174840.0
54	174840.0
55	166739.5
56	158639.0
57	147231.0
58	135823.0
59	124052.0
60	112281.0
61	112281.0
62	98004.5
63	83728.0
64	70627.0
65	57526.0
66	47278.0
67	37030.0
68	37030.0
69	29946.0
70	22862.0
71	18740.5
72	14619.0
73	11465.5
74	8312.0
75	8312.0
76	6599.5
77	4887.0
78	3866.5
79	2846.0
80	2217.5
81	1589.0
82	1589.0
83	1243.5
84	898.0
85	685.5
86	473.0
87	337.0
88	201.0
89	201.0
90	132.0
91	63.0
92	47.0
93	31.0
94	23.0
95	15.0
96	15.0
97	10.5
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2052141.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.48459071065571
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.22856775736913	50.557898332852616
2	9.429304993029046	11.595139163646664
3	3.1292183648446694	5.771961312202254
4	1.4883742107754545	3.660483166952961
5	0.889133927744211	2.7334017817155263
6	0.5522586622829461	2.0373238690128708
7	0.40287649098587097	1.7339487308648014
8	0.3013942939259782	1.482488384365267
9	0.23158630200315358	1.2815090093572383
>10	1.2740766356646027	14.019230309324893
>50	0.055362963810994925	2.260969154850542
>100	0.01721147977389435	1.5680811701664275
>500	7.923972375063309E-5	0.04136346663425302
>1k	4.754383425037986E-4	0.7259799956619603
>5k	0.0	0.0
>10k+	7.923972375063309E-5	0.5302221523917263
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10883	0.5303241833772631	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4624	0.22532564770159558	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4159	0.20266638598419895	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2840	0.13839205005893845	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.872959509117551E-5	4.872959509117551E-5
10	0.0	0.0	0.0	9.745919018235102E-5	4.872959509117551E-5
11	0.0	0.0	0.0	1.4618878527352652E-4	4.872959509117551E-5
12	0.0	0.0	0.0	2.4364797545587755E-4	1.4618878527352652E-4
13	0.0	4.872959509117551E-5	0.0	2.4364797545587755E-4	2.4364797545587755E-4
14	0.0	4.872959509117551E-5	0.0	2.4364797545587755E-4	2.9237757054705304E-4
15	0.0	4.872959509117551E-5	0.0	2.4364797545587755E-4	4.385663558205796E-4
16	0.0	4.872959509117551E-5	0.0	3.8983676072940407E-4	4.872959509117551E-4
17	0.0	4.872959509117551E-5	0.0	6.822143312764571E-4	4.872959509117551E-4
18	0.0	4.872959509117551E-5	0.0	6.822143312764571E-4	4.872959509117551E-4
19	0.0	4.872959509117551E-5	0.0	7.796735214588081E-4	4.872959509117551E-4
20	0.0	4.872959509117551E-5	0.0	0.0010720510920058611	5.360255460029306E-4
21	0.0	4.872959509117551E-5	0.0	0.0018517246134646694	5.360255460029306E-4
22	0.0	4.872959509117551E-5	0.0	0.003167423680926408	5.360255460029306E-4
23	0.0	4.872959509117551E-5	0.0	0.0038496380122028653	6.822143312764571E-4
24	0.0	4.872959509117551E-5	0.0	0.0047755003189352	6.822143312764571E-4
25	0.0	4.872959509117551E-5	0.0	0.005360255460029306	6.822143312764571E-4
26	0.0	4.872959509117551E-5	0.0	0.006529765742217518	6.822143312764571E-4
27	0.0	4.872959509117551E-5	0.0	0.01247477634334093	6.822143312764571E-4
28	0.0	4.872959509117551E-5	0.0	0.05335890662483718	6.822143312764571E-4
29	0.0	4.872959509117551E-5	0.0	0.1301567484885298	7.309439263676326E-4
30	0.0	4.872959509117551E-5	0.0	0.22318154551758382	7.309439263676326E-4
31	0.0	4.872959509117551E-5	0.0	0.5150718201137251	7.309439263676326E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGT	205	0.0	17.146343	6
TAGATCG	110	7.8149606E-7	16.818182	5
CGTATAC	70	0.0025934242	15.857142	3
CTTATAC	1300	0.0	15.796155	37
GGGTATA	260	0.0	15.653846	1
GTCTTAG	370	0.0	15.0	1
AACGCAG	4580	0.0	14.9454155	5
GTATTAG	310	0.0	14.919354	1
CTATCGG	75	0.0041058674	14.8	1
CGTCGAA	100	1.0940284E-4	14.8	22
TATACTG	430	0.0	14.627907	5
CGCCTTA	165	1.6470949E-8	14.575757	25
CGGTGAT	205	9.276846E-11	14.439025	14
GGTATCA	3270	0.0	14.426605	1
TAATCGA	90	8.27792E-4	14.388888	20
TTAGTAC	155	1.2114833E-7	14.32258	3
CCCTATA	260	0.0	14.230769	2
TTATACT	405	0.0	14.160494	4
ACTATAC	275	0.0	14.127274	3
TAGACGG	80	0.006300843	13.875001	5
>>END_MODULE