FastQCFastQC Report
Thu 9 Feb 2017
SRR2727385.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727385.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3573324
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT220320.6165687746199338No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT87290.2442823544688363No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT85490.23924502787880417No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62870.17594262373073363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG953.6106976E-617.5263165
CTTATAC31850.016.4379937
AACGCAG89450.016.2560085
TAGATCG700.002593928515.8571435
TAGACAG7100.014.8521135
ACGCAGA100850.014.5468516
CGCAGAG102950.014.2680917
GTATAGA3950.014.0506321
TAAGTCG800.00630204913.8750015
CGATCGA800.00630204913.87500120
CTAATAC4450.013.7191023
AGAGTAC108300.013.54616710
TAATACC4150.013.3734944
CAGAGTA113000.013.3265499
TTAGCGC1254.8499744E-513.324
TATACTG7400.013.05
GCAGAGT116150.012.9969868
CGTCGAA1153.580455E-412.86956522
TACACCG2305.7661964E-1012.8695655
TTATACT8700.012.758624
Produced by FastQC (version 0.11.5)