FastQCFastQC Report
Thu 9 Feb 2017
SRR2727384.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727384.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3452142
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT159030.46067050544270777No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT66740.19332924311919963No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64750.18756470620269966No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39730.1150879656746449No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC25550.020.7808237
TAGATCG1900.017.5263165
AACGCAG68700.016.8573535
ACGACCG2003.6379788E-1215.72517
CGACCGA1954.1836756E-1115.17948718
ACGCAGA77600.015.067016
CGACGCG2151.2732926E-1114.62790717
CGCAGAG80400.014.5422887
GGTATCA34600.014.3829491
ATCAACG80550.014.2855382
TCAACGC80900.014.2694673
CAACGCA81300.014.2675284
TATCAAC81000.014.2061731
AGAGTAC82800.014.18780210
TATACCG1702.4414476E-814.1470595
CAGAGTA87650.013.6560189
TCTATAC4750.013.6315783
TAGACAG6800.013.60294155
ACTATAC4250.013.4941173
GCAGAGT88850.013.4924038
Produced by FastQC (version 0.11.5)