FastQCFastQC Report
Thu 9 Feb 2017
SRR2727383.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727383.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13636305
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT673420.4938434568601978No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT263820.19346883191597725No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT239390.17555342154637932No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227530.16685605081435184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC156950.020.19146237
GGTATCA129700.017.1306861
AACGCAG277350.016.5489445
TCTTATA268250.015.12413837
CAACGCA309600.014.8848514
ATCAACG307950.014.8684852
TCAACGC309800.014.8513563
TATCAAC310200.014.7308181
ACGCAGA314800.014.5860876
CGCAGAG318450.014.4014767
TATACAC48950.014.323837
AGAGTAC324200.014.14605210
CAGAGTA335700.013.8212689
GCAGAGT344250.013.5639798
TCGTTAC18750.013.41866723
CTCGTTA20450.013.11735922
TACTTAC35550.012.90576631
GGAGTGT50350.012.6395236
GAGTACT212100.012.61244711
TATACCG5800.012.4396555
Produced by FastQC (version 0.11.5)