FastQCFastQC Report
Thu 9 Feb 2017
SRR2727382.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727382.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8847897
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT996321.1260528914384966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT346550.39167499350410606No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315580.3566723256385105No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT287830.32530894064431354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC57500.018.59652137
CGCGTAA1201.04349965E-716.95833210
AACGCAG377900.016.605455
CGCTACG12200.015.61885210
ACCGCTA12600.015.2698428
CGCAGAG422700.014.8323867
TCTTATA97000.014.66649437
ACGCAGA428000.014.6660056
AGAGTAC431750.014.431510
CAGAGTA439850.014.2540649
GAGTACT271800.014.18469511
GCAGAGT446550.014.1686278
AGTACTT275750.013.97479512
TACGGAC13800.013.40579713
GACCGTG19000.013.3394747
TATTCCG3750.013.325
CGAACGT2651.8189894E-1213.2641524
GTACTTT293550.013.1400113
CCGCTAC14550.012.9690719
ATCAACG484150.012.9574522
Produced by FastQC (version 0.11.5)