##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727382.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8847897
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13272961925303	33.0	31.0	34.0	30.0	34.0
2	32.26788930748177	33.0	31.0	34.0	30.0	34.0
3	32.34566372099494	34.0	31.0	34.0	30.0	34.0
4	35.912667382995075	37.0	35.0	37.0	35.0	37.0
5	35.805740392321475	37.0	35.0	37.0	35.0	37.0
6	35.83348709868571	37.0	35.0	37.0	35.0	37.0
7	35.77364123926849	37.0	35.0	37.0	35.0	37.0
8	35.759213743107544	37.0	35.0	37.0	35.0	37.0
9	37.481195362016535	39.0	37.0	39.0	35.0	39.0
10	37.32539427165574	39.0	37.0	39.0	34.0	39.0
11	37.438900904926896	39.0	37.0	39.0	35.0	39.0
12	37.34403180778438	39.0	37.0	39.0	34.0	39.0
13	37.405309645896644	39.0	37.0	39.0	34.0	39.0
14	38.53777829918228	40.0	38.0	41.0	34.0	41.0
15	38.512090274106946	40.0	38.0	41.0	34.0	41.0
16	38.45503050046808	40.0	38.0	41.0	34.0	41.0
17	38.43596608323989	40.0	38.0	41.0	34.0	41.0
18	38.40637758328335	40.0	38.0	41.0	34.0	41.0
19	38.42414338684096	40.0	38.0	41.0	34.0	41.0
20	38.314548643592936	40.0	38.0	41.0	34.0	41.0
21	38.23787483059534	40.0	38.0	41.0	34.0	41.0
22	38.16279088691923	40.0	38.0	41.0	34.0	41.0
23	38.05419852875774	40.0	38.0	41.0	33.0	41.0
24	37.9341150784192	40.0	37.0	41.0	33.0	41.0
25	37.858735245222675	40.0	37.0	41.0	33.0	41.0
26	37.49323132943342	40.0	37.0	41.0	32.0	41.0
27	37.27479320792274	39.0	37.0	41.0	31.0	41.0
28	37.12232680827998	39.0	36.0	41.0	31.0	41.0
29	37.05638345473506	39.0	36.0	40.0	31.0	41.0
30	36.95318797223792	39.0	36.0	40.0	31.0	41.0
31	36.718958979744	39.0	36.0	40.0	30.0	41.0
32	36.59547144366622	39.0	35.0	40.0	30.0	41.0
33	36.402675460620756	39.0	35.0	40.0	30.0	41.0
34	36.24350057420424	39.0	35.0	40.0	30.0	41.0
35	36.08183277902082	39.0	35.0	40.0	29.0	41.0
36	35.92510107204006	38.0	35.0	40.0	28.0	41.0
37	35.67196069303248	38.0	35.0	40.0	27.0	41.0
38	35.54659802210627	38.0	35.0	40.0	26.0	41.0
39	35.359609746813284	38.0	34.0	40.0	25.0	41.0
40	35.20498938900396	38.0	34.0	40.0	25.0	41.0
41	35.031474597862065	38.0	34.0	40.0	24.0	41.0
42	34.80074485496384	38.0	34.0	40.0	24.0	41.0
43	31.553732146746285	35.0	29.0	38.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	13.0
10	66.0
11	119.0
12	85.0
13	70.0
14	105.0
15	143.0
16	273.0
17	462.0
18	1069.0
19	1964.0
20	4099.0
21	7727.0
22	13642.0
23	21911.0
24	33017.0
25	47582.0
26	65443.0
27	86927.0
28	113768.0
29	144190.0
30	180322.0
31	224978.0
32	280487.0
33	354053.0
34	456854.0
35	600399.0
36	846625.0
37	1354658.0
38	2319944.0
39	1686901.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.48515777251928	18.887505132575573	13.337677868537575	25.28965922636758
2	18.47608533417602	22.459642104784898	34.29293989294857	24.77133266809051
3	18.647900173340627	22.66391663465341	31.5272318382549	27.160951353751067
4	14.390843383461629	15.847539816523632	33.64054757870712	36.121069221307614
5	14.326590827176219	37.979601254399775	33.6490919819704	14.0447159364536
6	35.4406702519254	36.11958864349348	13.935254897293673	14.504486207287448
7	29.452558048539668	31.607239550822076	20.127336473288512	18.81286592734974
8	28.75486683445795	34.93405269071283	18.38617696385932	17.924903510969894
9	27.11078123988107	13.647853269539642	18.33810904444299	40.9032564461363
10	15.554046345702261	28.67385323314681	33.08056140346118	22.691539017689742
11	37.11088634960375	21.494113233913097	20.95484384594441	20.44015657053874
12	21.627082684167775	26.137917292662877	29.88452510240569	22.35047492076366
13	32.319307062457895	19.78329991861343	23.79265943082294	24.104733588105738
14	22.057704785668278	20.657812811338104	26.794174932190103	30.49030747080351
15	25.28755703191391	28.815762660889927	22.184469371648426	23.712210935547734
16	24.138504324813002	26.473759809816954	24.310002704597487	25.077733160772553
17	24.23232322889835	26.597348499875167	25.7494747056843	23.420853565542185
18	23.9881070044102	25.385636835510176	26.490373927273343	24.13588223280628
19	25.099252398620823	25.60094223520007	26.79016267933499	22.509642686844117
20	24.357143850114895	25.616652182998966	26.473895435265575	23.552308531620564
21	24.05961552219697	25.915909735386837	26.768360888468752	23.25611385394744
22	24.62272108276125	25.657690183328306	25.903296568664846	23.816292165245596
23	23.9928991035949	25.608865021823828	26.2146134838595	24.183622390721773
24	23.805781192977268	25.751316951361435	26.356172545860336	24.086729309800962
25	24.037565084674924	25.3533127702549	26.47386152890342	24.135260616166757
26	23.60584667746471	25.781595332766642	26.6878219762278	23.924736013540844
27	24.095669287289397	26.13301217227099	26.351776020900786	23.419542519538826
28	23.730655996560536	26.197332541280716	26.359438858748018	23.71257260341073
29	23.737459873233156	26.434179783060312	26.409948036239573	23.41841230746696
30	23.787347434085184	26.436485415686917	26.210940294625946	23.56522685560196
31	23.7885680631228	25.626304194092675	26.461417893992213	24.12370984879232
32	23.307120324750617	26.250011725950245	26.336676387620695	24.106191561678443
33	23.56653790160532	25.691065345810422	26.560752232988243	24.181644519596013
34	23.752028306839467	25.71369219148912	26.930625435626116	23.603654066045298
35	23.562876014492485	25.88323530438928	26.712324973945783	23.84156370717245
36	23.34278981773861	25.41284104007992	27.128005671856258	24.116363470325208
37	23.14873240499974	25.23132898133873	27.202814408892873	24.41712420476866
38	22.86176025783302	25.105807628637628	27.698920997837114	24.33351111569224
39	22.73079128294554	24.953364624384754	28.077869803412042	24.237974289257664
40	22.83289464151764	24.77957191409439	28.394385694137263	23.99314775025071
41	22.454477035616485	24.609893175745604	28.774724660560587	24.160905128077328
42	21.987880283868584	24.742840021758845	28.740004545712956	24.529275148659618
43	22.074488434935443	23.9382985584032	28.705431358434662	25.28178164822669
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2926.0
1	2714.5
2	2503.0
3	5631.0
4	8759.0
5	8759.0
6	10098.0
7	11437.0
8	12031.0
9	12625.0
10	19117.0
11	25609.0
12	25609.0
13	46822.5
14	68036.0
15	110841.5
16	153647.0
17	116081.5
18	78516.0
19	78516.0
20	87940.0
21	97364.0
22	83311.0
23	69258.0
24	82326.0
25	95394.0
26	95394.0
27	112675.5
28	129957.0
29	145876.5
30	161796.0
31	181763.0
32	201730.0
33	201730.0
34	226010.0
35	250290.0
36	282681.5
37	315073.0
38	352053.0
39	389033.0
40	389033.0
41	436336.5
42	483640.0
43	526444.0
44	569248.0
45	608147.0
46	647046.0
47	647046.0
48	687206.5
49	727367.0
50	739948.5
51	752530.0
52	744125.5
53	735721.0
54	735721.0
55	701079.5
56	666438.0
57	625205.5
58	583973.0
59	530942.5
60	477912.0
61	477912.0
62	420535.5
63	363159.0
64	310254.0
65	257349.0
66	213890.5
67	170432.0
68	170432.0
69	140521.0
70	110610.0
71	92416.5
72	74223.0
73	62312.5
74	50402.0
75	50402.0
76	43056.5
77	35711.0
78	30465.5
79	25220.0
80	20762.5
81	16305.0
82	16305.0
83	13392.0
84	10479.0
85	8441.5
86	6404.0
87	5859.5
88	5315.0
89	5315.0
90	4011.5
91	2708.0
92	1920.5
93	1133.0
94	781.5
95	430.0
96	430.0
97	280.0
98	130.0
99	94.5
100	59.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	8847897.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.37614768810612
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.65089941568601	40.92156371870771
2	10.385249610146863	10.671082354975034
3	3.6193825966757722	5.578498044797266
4	1.7575842480293036	3.611916316041698
5	1.0087659555600732	2.591325435779391
6	0.6830042876411547	2.105407749207701
7	0.46940357812059474	1.6881303288394396
8	0.344379388970631	1.4154309078795906
9	0.2653971207089566	1.2271573502587334
>10	1.628446559408865	15.672892541330533
>50	0.12617439581587728	4.450222910415519
>100	0.05667557127330683	5.164418333954282
>500	0.0027477418657769442	0.9283989634221455
>1k	0.0017577024443942468	1.6135739442538928
>5k	4.394255092503499E-5	0.16302122278748848
>10k+	8.788510185006998E-5	2.196959877349627
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	99632	1.1260528914384966	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	34655	0.39167499350410606	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31558	0.3566723256385105	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	28783	0.32530894064431354	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.520848287451809E-5
2	0.0	0.0	0.0	0.0	5.651060359314762E-5
3	0.0	0.0	0.0	0.0	5.651060359314762E-5
4	0.0	0.0	0.0	0.0	5.651060359314762E-5
5	0.0	0.0	0.0	0.0	5.651060359314762E-5
6	0.0	0.0	0.0	0.0	6.781272431177714E-5
7	1.1302120718629523E-5	0.0	0.0	0.0	6.781272431177714E-5
8	1.1302120718629523E-5	0.0	0.0	0.0	6.781272431177714E-5
9	1.1302120718629523E-5	0.0	0.0	0.0	1.2432332790492475E-4
10	1.1302120718629523E-5	0.0	0.0	1.1302120718629523E-5	1.2432332790492475E-4
11	3.390636215588857E-5	0.0	0.0	2.2604241437259046E-5	1.2432332790492475E-4
12	3.390636215588857E-5	0.0	0.0	6.781272431177714E-5	1.2432332790492475E-4
13	3.390636215588857E-5	0.0	0.0	6.781272431177714E-5	1.2432332790492475E-4
14	5.651060359314762E-5	0.0	0.0	1.3562544862355427E-4	1.8083393149807237E-4
15	5.651060359314762E-5	0.0	0.0	2.3734453509122E-4	2.034381729353314E-4
16	5.651060359314762E-5	0.0	0.0	4.2948058730792185E-4	2.59948776528479E-4
17	5.651060359314762E-5	0.0	0.0	6.781272431177714E-4	2.59948776528479E-4
18	5.651060359314762E-5	0.0	0.0	8.250548124599552E-4	2.938551386843676E-4
19	5.651060359314762E-5	0.0	0.0	0.001141514192581582	2.938551386843676E-4
20	7.911484503040667E-5	0.0	0.0	0.0017405265906689465	2.938551386843676E-4
21	7.911484503040667E-5	0.0	0.0	0.002723811093189715	3.1645938012162667E-4
22	1.2432332790492475E-4	0.0	0.0	0.004712984339668511	3.1645938012162667E-4
23	1.2432332790492475E-4	0.0	0.0	0.005741477325063798	4.2948058730792185E-4
24	1.2432332790492475E-4	0.0	0.0	0.007527212398607262	4.407827080265514E-4
25	1.3562544862355427E-4	0.0	0.0	0.008352267211067218	4.6338694946381046E-4
26	1.3562544862355427E-4	0.0	0.0	0.009753730180177278	4.6338694946381046E-4
27	1.3562544862355427E-4	0.0	0.0	0.014602339968469344	4.6338694946381046E-4
28	1.469275693421838E-4	0.0	0.0	0.04297066297222945	4.6338694946381046E-4
29	1.469275693421838E-4	0.0	0.0	0.11280646689264127	4.6338694946381046E-4
30	1.469275693421838E-4	0.0	0.0	0.2003752982205828	4.6338694946381046E-4
31	1.469275693421838E-4	0.0	0.0	0.5416767396817571	4.6338694946381046E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	5750	0.0	18.596521	37
CGCGTAA	120	1.04349965E-7	16.958332	10
AACGCAG	37790	0.0	16.60545	5
CGCTACG	1220	0.0	15.618852	10
ACCGCTA	1260	0.0	15.269842	8
CGCAGAG	42270	0.0	14.832386	7
TCTTATA	9700	0.0	14.666494	37
ACGCAGA	42800	0.0	14.666005	6
AGAGTAC	43175	0.0	14.4315	10
CAGAGTA	43985	0.0	14.254064	9
GAGTACT	27180	0.0	14.184695	11
GCAGAGT	44655	0.0	14.168627	8
AGTACTT	27575	0.0	13.974795	12
TACGGAC	1380	0.0	13.405797	13
GACCGTG	1900	0.0	13.339474	7
TATTCCG	375	0.0	13.32	5
CGAACGT	265	1.8189894E-12	13.264152	4
GTACTTT	29355	0.0	13.14001	13
CCGCTAC	1455	0.0	12.969071	9
ATCAACG	48415	0.0	12.957452	2
>>END_MODULE