FastQCFastQC Report
Thu 9 Feb 2017
SRR2727381.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727381.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6944706
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT372230.5359910124345076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT157910.22738183589053304No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT122470.17635015794765105No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120920.17411824201053291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC47700.019.43081937
ATACCCG2400.016.9583345
GGTATCA86450.016.606131
TCTTATA80700.015.61152537
AACGCAG161100.015.5602125
CAACGCA181100.013.8213694
CGCAGAG182150.013.7315417
TCAACGC182250.013.7138553
ACGCAGA182750.013.6763336
ATCAACG182000.013.6513752
CAGAGTA188750.013.3592059
AGAGTAC186150.013.34703310
TATCAAC186600.013.3148451
TCTATAC6850.013.2335773
TCGTTAC10100.013.18811923
TACTTAC15050.013.029931
GCAGAGT194700.012.96995458
GTATACG2602.3646862E-1112.8076931
TATACTG10550.012.8009485
TATACCG3500.012.6857155
Produced by FastQC (version 0.11.5)