##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727381.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6944706
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.122016108385296	33.0	31.0	34.0	30.0	34.0
2	32.26767166241451	34.0	31.0	34.0	30.0	34.0
3	32.349260861438914	34.0	31.0	34.0	30.0	34.0
4	35.92920175454512	37.0	35.0	37.0	35.0	37.0
5	35.81402841243387	37.0	35.0	37.0	35.0	37.0
6	35.83169856290532	37.0	35.0	37.0	35.0	37.0
7	35.78240864336086	37.0	35.0	37.0	35.0	37.0
8	35.759060930729106	37.0	35.0	37.0	35.0	37.0
9	37.48176380684798	39.0	37.0	39.0	35.0	39.0
10	37.32174680972816	39.0	37.0	39.0	34.0	39.0
11	37.43253753290636	39.0	37.0	39.0	35.0	39.0
12	37.33513139361119	39.0	37.0	39.0	34.0	39.0
13	37.396582375121426	39.0	37.0	39.0	34.0	39.0
14	38.56442518948966	40.0	38.0	41.0	34.0	41.0
15	38.53293616749219	40.0	38.0	41.0	34.0	41.0
16	38.46837807100833	40.0	38.0	41.0	34.0	41.0
17	38.43316678920605	40.0	38.0	41.0	34.0	41.0
18	38.388299519086914	40.0	38.0	41.0	34.0	41.0
19	38.40235151783243	40.0	38.0	41.0	34.0	41.0
20	38.28897594800989	40.0	38.0	41.0	34.0	41.0
21	38.21329225456052	40.0	38.0	41.0	34.0	41.0
22	38.142565574410206	40.0	38.0	41.0	33.0	41.0
23	38.03600425993555	40.0	38.0	41.0	33.0	41.0
24	37.93361173244771	40.0	37.0	41.0	33.0	41.0
25	37.85139687698803	40.0	37.0	41.0	33.0	41.0
26	37.49900067763848	40.0	37.0	41.0	32.0	41.0
27	37.2916649027331	39.0	37.0	41.0	31.0	41.0
28	37.14073914720076	39.0	36.0	41.0	31.0	41.0
29	37.10884766036172	39.0	36.0	40.0	31.0	41.0
30	37.03180537808224	39.0	36.0	40.0	31.0	41.0
31	36.82347920847909	39.0	36.0	40.0	30.0	41.0
32	36.713465768025316	39.0	36.0	40.0	30.0	41.0
33	36.540760400800266	39.0	36.0	40.0	30.0	41.0
34	36.411416696401545	39.0	35.0	40.0	30.0	41.0
35	36.257439407802146	39.0	35.0	40.0	29.0	41.0
36	36.111797245268555	39.0	35.0	40.0	29.0	41.0
37	35.884803042778195	38.0	35.0	40.0	27.0	41.0
38	35.7839287941059	38.0	35.0	40.0	27.0	41.0
39	35.61951679451945	38.0	35.0	40.0	26.0	41.0
40	35.499443892945216	38.0	35.0	40.0	26.0	41.0
41	35.364282663657754	38.0	34.0	40.0	25.0	41.0
42	35.17265554510155	38.0	34.0	40.0	25.0	41.0
43	31.807671627855807	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	19.0
11	30.0
12	41.0
13	25.0
14	57.0
15	80.0
16	158.0
17	305.0
18	652.0
19	1316.0
20	2710.0
21	5232.0
22	9455.0
23	15838.0
24	24125.0
25	35168.0
26	48234.0
27	64799.0
28	85424.0
29	109067.0
30	138535.0
31	174007.0
32	217700.0
33	273302.0
34	353390.0
35	464953.0
36	655035.0
37	1034754.0
38	1879538.0
39	1350752.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.10258864810116	18.756272763742626	12.766083978213045	26.37505460994317
2	18.524671886758057	21.37073909248282	34.73033991647739	25.374249104281738
3	18.37022330390948	22.16936181315667	31.243108059578045	28.217306823355802
4	14.733697870003423	15.807839813521262	33.721355518865735	35.73710679760958
5	14.251935215112058	37.34025601659739	34.196206434080864	14.211602334209683
6	35.910879452636294	35.65210679904952	14.183465793944336	14.253547954369846
7	29.914254109533218	31.462930180197695	20.092902420923217	18.52991328934587
8	28.06905000730053	35.3833841202205	18.637736428295167	17.909829444183814
9	27.41326990660224	13.520097179059848	18.025212298403993	41.04142061593392
10	15.432273158863744	28.051525867329737	33.70206024560291	22.814140728203615
11	37.45561007190225	21.070366405719696	21.085802624329958	20.388220898048097
12	21.591684370799857	25.20989369456389	29.94273623678238	23.255685697853874
13	31.8636814863005	19.730180082497373	23.58370534332195	24.822433087880178
14	22.295069078518225	20.120318988305623	25.875854211826965	31.708757721349183
15	25.71597703344101	28.254241432250694	21.55780244692864	24.47197908737965
16	24.772250977939166	26.369813207355357	23.96491658538173	24.893019229323745
17	24.43298535603955	26.294705636206917	25.015313823220165	24.25699518453337
18	24.300884731477474	25.81269819053535	25.60577798397801	24.280639094009164
19	25.55450151525493	25.44076019920786	25.799105102505422	23.205633183031793
20	24.923373286068554	25.31516812950757	25.43775071255716	24.323707871866716
21	24.65979121362373	25.511346340651425	25.7885503000415	24.040312145683345
22	25.365638228601757	25.01722895108879	24.96465653117641	24.652476289133045
23	24.60851474490065	25.212672789892043	25.410478139751348	24.768334325455964
24	24.442373802433103	24.975499322793507	25.564609934531425	25.01751694024196
25	24.758485096417328	24.735575559282136	25.453546917608893	25.05239242669164
26	24.282813412115644	25.135333302806483	25.835564529297567	24.746288755780302
27	24.936361596876814	25.453575716524213	25.33158351123863	24.27847917536034
28	24.483628248625642	25.73481152405876	25.146492882492073	24.635067344823526
29	24.49557979848247	25.867315333435283	25.48858079809282	24.148524069989428
30	25.048821361192253	25.55044086819514	24.998610452335924	24.402127318276683
31	24.642252674195277	25.03767618096432	25.602178119563305	24.717893025277096
32	24.180202876838848	25.5025770709372	25.47069667168056	24.846523380543395
33	24.3526363823033	24.885517111883498	25.602883692988588	25.15896281282462
34	24.573567261162676	24.879296546174885	26.156211652444323	24.390924540218116
35	24.44676563701905	24.894142387021134	25.8786045082398	24.780487467720018
36	24.14669533886676	24.529375325607734	26.417691404070958	24.90623793145455
37	23.852917603711372	24.280955882077656	26.71555858520145	25.150567929009522
38	23.783713810203054	24.086577603141155	26.965173183717205	25.164535402938583
39	23.2573560349423	24.026402269584917	27.742859092955126	24.973382602517656
40	23.693357213393913	23.67951933458378	27.83654196448345	24.790581487538855
41	23.07046547398839	23.78388660369496	28.21393735026364	24.931710572053014
42	22.665480727333886	23.978538472326978	28.23690448522947	25.119076315109666
43	22.66710786604933	23.187360271262744	28.0995912569949	26.04594060569303
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1108.0
1	1180.5
2	1253.0
3	2499.5
4	3746.0
5	3746.0
6	4347.5
7	4949.0
8	5304.0
9	5659.0
10	8274.5
11	10890.0
12	10890.0
13	19061.5
14	27233.0
15	43116.0
16	58999.0
17	46807.0
18	34615.0
19	34615.0
20	40619.0
21	46623.0
22	43127.0
23	39631.0
24	48335.0
25	57039.0
26	57039.0
27	70114.0
28	83189.0
29	96079.5
30	108970.0
31	127879.5
32	146789.0
33	146789.0
34	170097.0
35	193405.0
36	221232.0
37	249059.0
38	282059.5
39	315060.0
40	315060.0
41	351343.0
42	387626.0
43	423943.0
44	460260.0
45	497037.5
46	533815.0
47	533815.0
48	566979.5
49	600144.0
50	616974.5
51	633805.0
52	625905.0
53	618005.0
54	618005.0
55	587758.0
56	557511.0
57	519634.0
58	481757.0
59	440599.0
60	399441.0
61	399441.0
62	348311.5
63	297182.0
64	251969.0
65	206756.0
66	171542.5
67	136329.0
68	136329.0
69	112190.0
70	88051.0
71	72731.5
72	57412.0
73	47175.0
74	36938.0
75	36938.0
76	30380.0
77	23822.0
78	19718.0
79	15614.0
80	12367.5
81	9121.0
82	9121.0
83	7359.0
84	5597.0
85	4402.5
86	3208.0
87	2701.0
88	2194.0
89	2194.0
90	1665.5
91	1137.0
92	794.5
93	452.0
94	329.5
95	207.0
96	207.0
97	138.5
98	70.0
99	52.5
100	35.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	6944706.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.08820345770761
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.8940893618258	45.37204142652066
2	9.906323955795857	11.112558271012817
3	3.3401490183161924	5.620288731551427
4	1.6663563306230502	3.7385173162009884
5	0.9554971420540211	2.6796059053392036
6	0.629660621084969	2.1189919814832163
7	0.42360980637757095	1.6631659104749725
8	0.3108345275326389	1.3947320177544853
9	0.24323852858557585	1.2278530872055085
>10	1.4804444197667044	15.488667819029518
>50	0.10723504341862122	4.092785296863396
>100	0.041277475905683744	3.8040134169089406
>500	9.500059247958065E-4	0.35718699749966876
>1k	2.3106654597660582E-4	0.21569938818514087
>5k	0.0	0.0
>10k+	1.0269624260854878E-4	1.1138924339699712
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	37223	0.5359910124345076	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15791	0.22738183589053304	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12247	0.17635015794765105	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12092	0.17411824201053291	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.3198372976480215E-5
2	0.0	0.0	0.0	0.0	4.3198372976480215E-5
3	0.0	0.0	0.0	0.0	4.3198372976480215E-5
4	0.0	0.0	0.0	0.0	4.3198372976480215E-5
5	0.0	0.0	0.0	0.0	4.3198372976480215E-5
6	0.0	0.0	0.0	0.0	5.759783063530695E-5
7	0.0	0.0	0.0	0.0	5.759783063530695E-5
8	1.4399457658826738E-5	0.0	1.4399457658826738E-5	0.0	8.639674595296043E-5
9	1.4399457658826738E-5	0.0	1.4399457658826738E-5	0.0	1.583940342470941E-4
10	2.8798915317653477E-5	0.0	1.4399457658826738E-5	2.8798915317653477E-5	1.583940342470941E-4
11	5.759783063530695E-5	0.0	1.4399457658826738E-5	2.8798915317653477E-5	1.583940342470941E-4
12	5.759783063530695E-5	0.0	1.4399457658826738E-5	5.759783063530695E-5	1.8719294956474758E-4
13	5.759783063530695E-5	0.0	1.4399457658826738E-5	5.759783063530695E-5	1.8719294956474758E-4
14	8.639674595296043E-5	0.0	1.4399457658826738E-5	5.759783063530695E-5	2.4479078020005457E-4
15	8.639674595296043E-5	1.4399457658826738E-5	1.4399457658826738E-5	1.0079620361178717E-4	2.5919023785888126E-4
16	1.0079620361178717E-4	1.4399457658826738E-5	1.4399457658826738E-5	2.303913225412278E-4	3.3118752615301497E-4
17	1.0079620361178717E-4	1.4399457658826738E-5	1.4399457658826738E-5	3.887853567883219E-4	3.3118752615301497E-4
18	1.0079620361178717E-4	1.4399457658826738E-5	1.4399457658826738E-5	4.607826450824556E-4	3.5998644147066847E-4
19	1.2959511892944063E-4	1.4399457658826738E-5	1.4399457658826738E-5	5.615788486942428E-4	3.5998644147066847E-4
20	1.4399457658826738E-4	1.4399457658826738E-5	1.4399457658826738E-5	8.351685442119509E-4	3.5998644147066847E-4
21	1.4399457658826738E-4	1.4399457658826738E-5	1.4399457658826738E-5	0.0012239539010002727	3.7438589912949517E-4
22	1.4399457658826738E-4	1.4399457658826738E-5	1.4399457658826738E-5	0.0020015246145769165	3.7438589912949517E-4
23	1.4399457658826738E-4	1.4399457658826738E-5	2.8798915317653477E-5	0.002577502920929986	4.751821027412824E-4
24	1.4399457658826738E-4	1.4399457658826738E-5	2.8798915317653477E-5	0.003239877973236016	4.895815604001091E-4
25	1.4399457658826738E-4	1.4399457658826738E-5	2.8798915317653477E-5	0.0036430627876831647	5.039810180589359E-4
26	1.4399457658826738E-4	1.4399457658826738E-5	2.8798915317653477E-5	0.004377435128283328	5.039810180589359E-4
27	1.4399457658826738E-4	1.4399457658826738E-5	2.8798915317653477E-5	0.009230052359307938	5.183804757177625E-4
28	1.4399457658826738E-4	1.4399457658826738E-5	2.8798915317653477E-5	0.038115364422914376	5.327799333765893E-4
29	1.4399457658826738E-4	2.8798915317653477E-5	2.8798915317653477E-5	0.09759952401152763	5.47179391035416E-4
30	1.4399457658826738E-4	2.8798915317653477E-5	2.8798915317653477E-5	0.17839488093520445	5.47179391035416E-4
31	1.583940342470941E-4	2.8798915317653477E-5	2.8798915317653477E-5	0.5401092573249321	5.47179391035416E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	4770	0.0	19.430819	37
ATACCCG	240	0.0	16.958334	5
GGTATCA	8645	0.0	16.60613	1
TCTTATA	8070	0.0	15.611525	37
AACGCAG	16110	0.0	15.560212	5
CAACGCA	18110	0.0	13.821369	4
CGCAGAG	18215	0.0	13.731541	7
TCAACGC	18225	0.0	13.713855	3
ACGCAGA	18275	0.0	13.676333	6
ATCAACG	18200	0.0	13.651375	2
CAGAGTA	18875	0.0	13.359205	9
AGAGTAC	18615	0.0	13.347033	10
TATCAAC	18660	0.0	13.314845	1
TCTATAC	685	0.0	13.233577	3
TCGTTAC	1010	0.0	13.188119	23
TACTTAC	1505	0.0	13.0299	31
GCAGAGT	19470	0.0	12.9699545	8
GTATACG	260	2.3646862E-11	12.807693	1
TATACTG	1055	0.0	12.800948	5
TATACCG	350	0.0	12.685715	5
>>END_MODULE