FastQCFastQC Report
Thu 9 Feb 2017
SRR2727380.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727380.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5768690
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT448780.7779582539536706No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT181990.3154789042226225No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151940.2633873548413938No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT130640.2264638938823199No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC39750.018.66289337
TCTTATA67600.015.46227937
AACGCAG190550.015.4271845
TACTTAC14650.015.02730431
GTACTTA16850.013.94362130
CGCAGAG216100.013.6459977
ACGCAGA217650.013.53181656
CTCGTTA9900.013.26767722
AGAGTAC220500.013.24784610
TCGTTAC8450.013.13609523
CAGAGTA226150.012.9659519
TTATACT8150.012.938654
GAGTACT131500.012.85855611
GCAGAGT230700.012.7583458
CGCTACG5100.012.69607810
CTCTTAT102450.012.67642837
TATACTG7300.012.6712325
AGTACTT134850.012.49796112
ATCAACG237900.012.37999252
CAACGCA239000.012.3539744
Produced by FastQC (version 0.11.5)