##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727380.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5768690
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12954102231183	33.0	31.0	34.0	30.0	34.0
2	32.268331978317434	34.0	31.0	34.0	30.0	34.0
3	32.34736482632972	34.0	31.0	34.0	30.0	34.0
4	35.92213778171474	37.0	35.0	37.0	35.0	37.0
5	35.809153724675795	37.0	35.0	37.0	35.0	37.0
6	35.83170702533851	37.0	35.0	37.0	35.0	37.0
7	35.778027593786454	37.0	35.0	37.0	35.0	37.0
8	35.758927590146115	37.0	35.0	37.0	35.0	37.0
9	37.48392824020705	39.0	37.0	39.0	35.0	39.0
10	37.32507779062491	39.0	37.0	39.0	34.0	39.0
11	37.4382681336664	39.0	37.0	39.0	35.0	39.0
12	37.34639961585733	39.0	37.0	39.0	34.0	39.0
13	37.4072175831948	39.0	37.0	39.0	34.0	39.0
14	38.559095739240625	40.0	38.0	41.0	34.0	41.0
15	38.53440174459019	40.0	38.0	41.0	34.0	41.0
16	38.46502082795227	40.0	38.0	41.0	34.0	41.0
17	38.44472939263507	40.0	38.0	41.0	34.0	41.0
18	38.40352645054597	40.0	38.0	41.0	34.0	41.0
19	38.4238983200692	40.0	38.0	41.0	34.0	41.0
20	38.31170144348197	40.0	38.0	41.0	34.0	41.0
21	38.2372587190506	40.0	38.0	41.0	34.0	41.0
22	38.16905761966755	40.0	38.0	41.0	34.0	41.0
23	38.05558783709993	40.0	38.0	41.0	33.0	41.0
24	37.95109288243951	40.0	37.0	41.0	33.0	41.0
25	37.87109811759689	40.0	37.0	41.0	33.0	41.0
26	37.52154371269734	40.0	37.0	41.0	32.0	41.0
27	37.31455113725993	39.0	37.0	41.0	31.0	41.0
28	37.15886882463783	39.0	36.0	41.0	31.0	41.0
29	37.114105628834274	39.0	36.0	40.0	31.0	41.0
30	37.026916509640834	39.0	36.0	40.0	31.0	41.0
31	36.80718984726168	39.0	36.0	40.0	30.0	41.0
32	36.69785947936187	39.0	36.0	40.0	30.0	41.0
33	36.51917246376561	39.0	35.0	40.0	30.0	41.0
34	36.376585325264486	39.0	35.0	40.0	30.0	41.0
35	36.22591888279661	39.0	35.0	40.0	29.0	41.0
36	36.06867729068471	39.0	35.0	40.0	29.0	41.0
37	35.83633008534	38.0	35.0	40.0	27.0	41.0
38	35.72476073423949	38.0	35.0	40.0	27.0	41.0
39	35.546737300843	38.0	35.0	40.0	26.0	41.0
40	35.40781997299214	38.0	34.0	40.0	26.0	41.0
41	35.25762365459056	38.0	34.0	40.0	25.0	41.0
42	35.05437248318076	38.0	34.0	40.0	24.0	41.0
43	31.73829656299784	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	8.0
10	26.0
11	44.0
12	31.0
13	27.0
14	29.0
15	60.0
16	128.0
17	267.0
18	522.0
19	1141.0
20	2433.0
21	4454.0
22	8057.0
23	13084.0
24	20049.0
25	29495.0
26	39763.0
27	54211.0
28	70544.0
29	90230.0
30	115360.0
31	144295.0
32	180191.0
33	229305.0
34	294600.0
35	390560.0
36	549275.0
37	870918.0
38	1547813.0
39	1111770.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.17536390410995	18.835818877422778	13.088690846622022	25.900126371845257
2	18.576245213384666	21.864045389854542	34.49150500373568	25.068204393025106
3	18.752732422785762	22.372774408054514	31.30787059107007	27.566622578089657
4	14.821319918387015	15.879445766716533	33.254534391690314	36.04469992320614
5	14.457043106840548	37.5391986742224	33.747644612555014	14.256113606382037
6	35.850444381653375	35.31630231473697	14.12587606544987	14.707377238159792
7	29.55369416626652	31.42169192658992	20.17763131664208	18.846982590501483
8	28.318561059790003	35.1594036080982	18.62870079688803	17.89333453522377
9	27.337783794934378	13.597679889194946	18.244246094000545	40.82029022187013
10	15.699907604672811	28.24828513926039	33.34762311720685	22.704184138859947
11	37.18861647965136	21.30013919971432	20.908646503798956	20.602597816835363
12	21.498329776777744	25.491610746980687	29.898001105970334	23.112058370271242
13	32.046929198830234	19.768630312947998	23.62896948874008	24.555470999481685
14	22.255971459724826	20.49120337546306	26.231345418110525	31.02147974670159
15	25.75983455515897	28.393604093823726	21.829184788920884	24.01737656209642
16	24.344643237892832	26.382176889380432	24.344053849314143	24.929126023412596
17	24.254570795102527	26.39552480719193	25.325489842581245	24.024414555124302
18	24.069537451310435	25.72150696258596	26.070719695459456	24.13823589064415
19	25.384619384990355	25.582307248266073	26.190382911891607	22.84269045485197
20	24.57750373134975	25.347141205368985	25.959862637791247	24.115492425490014
21	24.26176480275418	25.77900701892457	26.352291421449237	23.60693675687201
22	25.02110531160454	25.169613898476083	25.39113732927233	24.418143460647045
23	24.255714902343513	25.416810402361715	25.901669183124763	24.425805512170008
24	24.040397386581702	25.263846731233606	26.020881690643805	24.674874191540887
25	24.407829160519977	25.00661328655206	25.897231433826395	24.688326119101564
26	24.003768619911973	25.304861242327114	26.2954327585639	24.39593737919701
27	24.63275024312279	25.747162700717148	25.809291190894296	23.81079586526577
28	24.173287176117974	26.05225796497992	25.52643667799795	24.248018180904158
29	24.134196845384306	26.093601840279163	26.032478777677426	23.7397225366591
30	24.63462241860804	25.812220798829543	25.53489613759796	24.018260644964453
31	24.284109563869787	25.259426316893435	26.047854885597943	24.408609233638835
32	23.853110498224034	25.795475229211483	25.83408018111564	24.517334091448838
33	24.00234715333984	25.141375251573585	25.97425065309455	24.88202694199203
34	24.25011571084596	25.20379843604007	26.560917643347103	23.985168209766865
35	24.07073356342601	25.15371774180967	26.291619067760617	24.483929627003704
36	23.732961902962373	24.792699902404188	26.891547301033686	24.58279089359976
37	23.408278135937273	24.648403710374453	27.175147217132483	24.768170936555787
38	23.465206138655397	24.376868925180588	27.364774324846714	24.793150611317298
39	22.913885128166015	24.394949286579795	28.123768134533144	24.56739745072105
40	23.376399147813455	24.045407189500562	28.167746923478294	24.41044673920769
41	22.75450752250511	24.17789827499831	28.50851753170997	24.55907667078661
42	22.31246608848803	24.444128562983973	28.477089252499265	24.766316096028735
43	22.319642761181484	23.612969322324478	28.252844233266135	25.81454368322791
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1654.0
1	1589.5
2	1525.0
3	2989.0
4	4453.0
5	4453.0
6	5015.5
7	5578.0
8	5959.0
9	6340.0
10	9504.0
11	12668.0
12	12668.0
13	22971.0
14	33274.0
15	52379.5
16	71485.0
17	54785.0
18	38085.0
19	38085.0
20	44410.0
21	50735.0
22	44357.5
23	37980.0
24	45581.5
25	53183.0
26	53183.0
27	64306.5
28	75430.0
29	85421.5
30	95413.0
31	110014.5
32	124616.0
33	124616.0
34	141565.0
35	158514.0
36	180532.5
37	202551.0
38	227939.5
39	253328.0
40	253328.0
41	282989.5
42	312651.0
43	342642.0
44	372633.0
45	404151.5
46	435670.0
47	435670.0
48	465624.0
49	495578.0
50	508154.0
51	520730.0
52	514247.0
53	507764.0
54	507764.0
55	479251.5
56	450739.0
57	420959.5
58	391180.0
59	359348.0
60	327516.0
61	327516.0
62	285984.0
63	244452.0
64	206642.5
65	168833.0
66	139615.5
67	110398.0
68	110398.0
69	91044.0
70	71690.0
71	58994.0
72	46298.0
73	38521.5
74	30745.0
75	30745.0
76	25640.0
77	20535.0
78	16970.5
79	13406.0
80	11060.0
81	8714.0
82	8714.0
83	6918.0
84	5122.0
85	4103.0
86	3084.0
87	2660.0
88	2236.0
89	2236.0
90	1704.5
91	1173.0
92	826.0
93	479.0
94	329.0
95	179.0
96	179.0
97	114.0
98	49.0
99	36.5
100	24.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5768690.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.69123643298646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.69996616238544	45.749808618458
2	10.15292829932152	11.511641174079907
3	3.399970015385404	5.782455120218358
4	1.6238209457987998	3.6822566865246182
5	0.952723093485814	2.7005525073985273
6	0.6003825410733863	2.0421857151737126
7	0.42250104039398984	1.676647446191091
8	0.3242916827768441	1.470759716924249
9	0.25091860646519315	1.2802397440098374
>10	1.453581499874872	15.031055265076729
>50	0.08411854962687691	3.2282074553420967
>100	0.032567624686340294	3.153702318249548
>500	0.001527360706059634	0.5890911907645212
>1k	5.803905379953619E-4	0.5197124472946859
>5k	0.0	0.0
>10k+	1.2218748162682488E-4	1.5816845942940083
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	44878	0.7779582539536706	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	18199	0.3154789042226225	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15194	0.2633873548413938	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13064	0.2264638938823199	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.466991639349662E-5
2	1.733495819674831E-5	0.0	0.0	0.0	5.2004874590244924E-5
3	1.733495819674831E-5	0.0	0.0	0.0	5.2004874590244924E-5
4	1.733495819674831E-5	0.0	0.0	0.0	5.2004874590244924E-5
5	3.466991639349662E-5	0.0	0.0	0.0	5.2004874590244924E-5
6	3.466991639349662E-5	0.0	0.0	0.0	1.2134470737723816E-4
7	5.2004874590244924E-5	0.0	0.0	0.0	1.2134470737723816E-4
8	6.933983278699324E-5	0.0	0.0	0.0	1.3867966557398647E-4
9	8.667479098374155E-5	0.0	0.0	0.0	1.3867966557398647E-4
10	1.2134470737723816E-4	0.0	0.0	3.466991639349662E-5	1.5601462377073477E-4
11	1.2134470737723816E-4	0.0	0.0	3.466991639349662E-5	1.5601462377073477E-4
12	1.3867966557398647E-4	0.0	0.0	5.2004874590244924E-5	1.5601462377073477E-4
13	1.3867966557398647E-4	0.0	0.0	6.933983278699324E-5	1.906845401642314E-4
14	1.5601462377073477E-4	0.0	0.0	8.667479098374155E-5	2.600243729512246E-4
15	1.5601462377073477E-4	0.0	0.0	1.5601462377073477E-4	2.7735933114797295E-4
16	1.5601462377073477E-4	0.0	0.0	2.600243729512246E-4	3.6403412213171447E-4
17	1.5601462377073477E-4	1.733495819674831E-5	0.0	4.5070891311545604E-4	3.6403412213171447E-4
18	1.5601462377073477E-4	1.733495819674831E-5	0.0	5.547186622959459E-4	3.6403412213171447E-4
19	1.5601462377073477E-4	1.733495819674831E-5	0.0	7.627381606569256E-4	3.6403412213171447E-4
20	1.906845401642314E-4	1.733495819674831E-5	0.0	0.0011614421991821367	3.6403412213171447E-4
21	2.080194983609797E-4	1.733495819674831E-5	0.0	0.0018201706106585724	3.813690803284628E-4
22	2.2535445655772802E-4	1.733495819674831E-5	0.0	0.003189632308201689	3.813690803284628E-4
23	2.2535445655772802E-4	1.733495819674831E-5	0.0	0.0038483607196781247	4.333739549187077E-4
24	2.2535445655772802E-4	1.733495819674831E-5	0.0	0.005113812668040751	4.333739549187077E-4
25	2.2535445655772802E-4	1.733495819674831E-5	0.0	0.005720536204926942	4.680438713122043E-4
26	2.2535445655772802E-4	1.733495819674831E-5	0.0	0.006812638571322085	4.680438713122043E-4
27	2.2535445655772802E-4	1.733495819674831E-5	0.0	0.012983883689364484	4.680438713122043E-4
28	2.2535445655772802E-4	1.733495819674831E-5	0.0	0.04519223601892284	4.680438713122043E-4
29	2.2535445655772802E-4	1.733495819674831E-5	0.0	0.11714964749362507	4.680438713122043E-4
30	2.2535445655772802E-4	1.733495819674831E-5	0.0	0.2106717469650822	4.680438713122043E-4
31	2.2535445655772802E-4	1.733495819674831E-5	0.0	0.573093717984499	5.027137877057009E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	3975	0.0	18.662893	37
TCTTATA	6760	0.0	15.462279	37
AACGCAG	19055	0.0	15.427184	5
TACTTAC	1465	0.0	15.027304	31
GTACTTA	1685	0.0	13.943621	30
CGCAGAG	21610	0.0	13.645997	7
ACGCAGA	21765	0.0	13.5318165	6
CTCGTTA	990	0.0	13.267677	22
AGAGTAC	22050	0.0	13.247846	10
TCGTTAC	845	0.0	13.136095	23
CAGAGTA	22615	0.0	12.965951	9
TTATACT	815	0.0	12.93865	4
GAGTACT	13150	0.0	12.858556	11
GCAGAGT	23070	0.0	12.758345	8
CGCTACG	510	0.0	12.696078	10
CTCTTAT	10245	0.0	12.676428	37
TATACTG	730	0.0	12.671232	5
AGTACTT	13485	0.0	12.497961	12
ATCAACG	23790	0.0	12.3799925	2
CAACGCA	23900	0.0	12.353974	4
>>END_MODULE