##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727379.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5265878
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1473596615797	33.0	31.0	34.0	30.0	34.0
2	32.28326026542962	34.0	31.0	34.0	30.0	34.0
3	32.36108945174955	34.0	31.0	34.0	30.0	34.0
4	35.93262225216763	37.0	35.0	37.0	35.0	37.0
5	35.82212842758606	37.0	35.0	37.0	35.0	37.0
6	35.84500438483383	37.0	35.0	37.0	35.0	37.0
7	35.78803192933828	37.0	35.0	37.0	35.0	37.0
8	35.76561211634603	37.0	35.0	37.0	35.0	37.0
9	37.49503691502158	39.0	37.0	39.0	35.0	39.0
10	37.33522671812754	39.0	37.0	39.0	34.0	39.0
11	37.44727735811578	39.0	37.0	39.0	35.0	39.0
12	37.35341456828282	39.0	37.0	39.0	34.0	39.0
13	37.416313290964965	39.0	37.0	39.0	35.0	39.0
14	38.55826378810903	40.0	38.0	41.0	34.0	41.0
15	38.53251632491296	40.0	38.0	41.0	34.0	41.0
16	38.46935629727844	40.0	38.0	41.0	34.0	41.0
17	38.44538004868324	40.0	38.0	41.0	34.0	41.0
18	38.40809338917461	40.0	38.0	41.0	34.0	41.0
19	38.422421674030424	40.0	38.0	41.0	34.0	41.0
20	38.307893384541	40.0	38.0	41.0	34.0	41.0
21	38.23404473100212	40.0	38.0	41.0	34.0	41.0
22	38.16143936490743	40.0	38.0	41.0	34.0	41.0
23	38.053754188760166	40.0	38.0	41.0	33.0	41.0
24	37.945868286352244	40.0	37.0	41.0	33.0	41.0
25	37.86160161705227	40.0	37.0	41.0	33.0	41.0
26	37.50490668412751	40.0	37.0	41.0	32.0	41.0
27	37.28636231222979	39.0	37.0	41.0	31.0	41.0
28	37.12667972938226	39.0	36.0	41.0	31.0	41.0
29	37.07092967212685	39.0	36.0	40.0	31.0	41.0
30	36.97179976444574	39.0	36.0	40.0	31.0	41.0
31	36.751437651992696	39.0	36.0	40.0	30.0	41.0
32	36.632042937569004	39.0	36.0	40.0	30.0	41.0
33	36.4425550686894	39.0	35.0	40.0	30.0	41.0
34	36.29658738770629	39.0	35.0	40.0	30.0	41.0
35	36.13359481552744	39.0	35.0	40.0	29.0	41.0
36	35.969463022120905	38.0	35.0	40.0	28.0	41.0
37	35.728729757886526	38.0	35.0	40.0	27.0	41.0
38	35.613057879426755	38.0	35.0	40.0	26.0	41.0
39	35.43082312199409	38.0	35.0	40.0	26.0	41.0
40	35.283563348790075	38.0	34.0	40.0	25.0	41.0
41	35.11927735507735	38.0	34.0	40.0	24.0	41.0
42	34.897314369987306	38.0	34.0	40.0	24.0	41.0
43	31.61974641265901	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	10.0
11	20.0
12	24.0
13	30.0
14	29.0
15	78.0
16	164.0
17	284.0
18	552.0
19	1081.0
20	2320.0
21	4452.0
22	7854.0
23	12670.0
24	19569.0
25	27524.0
26	38075.0
27	50518.0
28	66162.0
29	84320.0
30	106339.0
31	132789.0
32	166386.0
33	209669.0
34	270422.0
35	357037.0
36	503110.0
37	799055.0
38	1393232.0
39	1012099.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.396747512950355	18.683854810157012	13.165990552762521	25.753407124130106
2	18.597259564311972	22.140790196810485	34.31646156633329	24.945488672544254
3	18.63457527880441	22.429497986850436	31.318006228021233	27.617920506323923
4	14.605446613081426	15.743319537596578	33.53476856091235	36.11646528840964
5	14.523086178601174	37.54029242606836	33.76454980536959	14.172071589960877
6	35.806393539690816	35.63939384847124	13.962059128601156	14.592153483236794
7	29.86643442935822	31.244837043319272	20.114043659955662	18.774684867366847
8	28.584331046028794	34.94763836154199	18.481058619284383	17.98697197314484
9	27.342847669467467	13.608025100467577	18.23154657210061	40.817580657964356
10	15.67831613265632	28.25844047279485	33.11423090318462	22.94901249136421
11	37.11553894716133	21.38663296035343	20.891862667536166	20.605965424949076
12	21.669282881221328	25.852687813884028	29.61646661772263	22.861562687172015
13	32.31385155523922	19.521284009997952	23.568890126204973	24.595974308557853
14	22.25571879941009	20.504045099411723	26.294817312516543	30.945418788661645
15	25.697006273217877	28.450735091090223	21.726443339553253	24.12581529613865
16	24.422840787424242	26.21135544727774	24.191730229982543	25.17407353531548
17	24.396425439404407	26.3806339607564	25.29124297980318	23.93169762003601
18	24.209998788426166	25.558396909309334	25.97623796069715	24.25536634156735
19	25.51802377495263	25.47968259044361	26.094489845757916	22.907803788845847
20	24.630574426524884	25.528753229755797	25.828152494227936	24.012519849491387
21	24.32321827433146	25.879236093202312	26.097908079146535	23.699637553319693
22	24.949609542796093	25.414185440680548	25.30888486212556	24.327320154397807
23	24.26537416932181	25.511529131514248	25.679041557742128	24.54405514142181
24	24.09463341156024	25.4967927475722	25.785880341322	24.622693499545566
25	24.425955177844987	25.125173807672716	25.763130099102185	24.685740915380112
26	23.960600682355345	25.548408071740365	26.173602958518977	24.317388287385313
27	24.556113909209444	25.992930333744912	25.63804174726418	23.812914009781462
28	24.03572585616302	26.21486863159382	25.597668612907476	24.151736899335685
29	24.09478533304418	26.320643964786118	25.837020910852853	23.747549791316853
30	24.42606911895794	26.15250486243699	25.47613142575654	23.945294592848523
31	24.121979278669198	25.42328173953138	25.968622896314724	24.4861160854847
32	23.625784721940008	26.039000523749316	25.832463266334692	24.502751487975985
33	23.915024237173743	25.44570914859782	25.867785011350435	24.771481602878
34	24.084131838982977	25.463237089807244	26.475584888218073	23.977046182991707
35	23.941306653895133	25.53439331484702	26.171627979227775	24.35267205203007
36	23.707803333081397	25.014916790704227	26.717861674729264	24.55941820148511
37	23.344748966838957	24.80158104688335	26.924569843813323	24.92910014246437
38	23.1781860498857	24.560443671501694	27.45019918805563	24.81117109055698
39	22.7773791948845	24.545764258116122	28.017246126856715	24.65961042014266
40	23.081393074431272	24.255670184535226	28.285121683411578	24.37781505762192
41	22.509560608886115	24.223652731795152	28.706115105591127	24.5606715537276
42	22.006871408718546	24.44705327392697	28.736290510338446	24.80978480701604
43	21.971872496856175	23.673013313259442	28.525879255083385	25.829234934800997
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1107.0
1	1103.5
2	1100.0
3	2594.5
4	4089.0
5	4089.0
6	4732.0
7	5375.0
8	5694.0
9	6013.0
10	9256.0
11	12499.0
12	12499.0
13	22877.0
14	33255.0
15	53959.5
16	74664.0
17	56448.5
18	38233.0
19	38233.0
20	43413.0
21	48593.0
22	41922.0
23	35251.0
24	42261.0
25	49271.0
26	49271.0
27	59894.0
28	70517.0
29	79946.0
30	89375.0
31	102073.0
32	114771.0
33	114771.0
34	130389.0
35	146007.0
36	166441.5
37	186876.0
38	210642.0
39	234408.0
40	234408.0
41	259160.5
42	283913.0
43	310594.5
44	337276.0
45	362433.5
46	387591.0
47	387591.0
48	411475.5
49	435360.0
50	448127.5
51	460895.0
52	458046.5
53	455198.0
54	455198.0
55	432513.0
56	409828.0
57	383114.0
58	356400.0
59	325396.5
60	294393.0
61	294393.0
62	258856.0
63	223319.0
64	190937.0
65	158555.0
66	132524.0
67	106493.0
68	106493.0
69	87913.5
70	69334.0
71	58366.0
72	47398.0
73	39447.5
74	31497.0
75	31497.0
76	26359.0
77	21221.0
78	17698.5
79	14176.0
80	11420.5
81	8665.0
82	8665.0
83	6980.0
84	5295.0
85	4241.0
86	3187.0
87	2818.5
88	2450.0
89	2450.0
90	1840.0
91	1230.0
92	852.5
93	475.0
94	352.0
95	229.0
96	229.0
97	143.5
98	58.0
99	48.0
100	38.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5265878.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.678230297992584
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.94896811568684	47.843496859574294
2	9.15876123292964	10.565222792744846
3	2.8609327779287894	4.950406188973572
4	1.412868491015003	3.259670168221624
5	0.8201928933954306	2.3653637297019263
6	0.5173090661396949	1.790248287122688
7	0.36331562638052606	1.466878165846479
8	0.2699691506035314	1.2457074273491142
9	0.21085978728463783	1.094581743443016
>10	1.290111902778237	14.030918693283134
>50	0.1002844185579982	3.966928231253523
>100	0.04402282954496588	4.474297520929035
>500	0.0017122320440406224	0.6657648028526564
>1k	5.597660515775739E-4	0.5527417168321611
>5k	0.0	0.0
>10k+	1.3170965919079967E-4	1.727773671871938
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	47232	0.8969444411739126	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16089	0.30553309438615933	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14599	0.2772377180025819	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13054	0.2478978814169261	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.899018549233385E-5	0.0	0.0	0.0
6	0.0	1.899018549233385E-5	0.0	0.0	1.899018549233385E-5
7	0.0	1.899018549233385E-5	0.0	0.0	3.79803709846677E-5
8	0.0	1.899018549233385E-5	0.0	0.0	3.79803709846677E-5
9	0.0	1.899018549233385E-5	0.0	0.0	5.6970556477001555E-5
10	0.0	1.899018549233385E-5	0.0	0.0	5.6970556477001555E-5
11	1.899018549233385E-5	1.899018549233385E-5	0.0	5.6970556477001555E-5	5.6970556477001555E-5
12	1.899018549233385E-5	1.899018549233385E-5	0.0	7.59607419693354E-5	5.6970556477001555E-5
13	1.899018549233385E-5	1.899018549233385E-5	0.0	1.1394111295400311E-4	5.6970556477001555E-5
14	3.79803709846677E-5	1.899018549233385E-5	0.0	1.3293129844633696E-4	7.59607419693354E-5
15	3.79803709846677E-5	1.899018549233385E-5	0.0	2.468724114003401E-4	7.59607419693354E-5
16	3.79803709846677E-5	1.899018549233385E-5	0.0	4.367742663236786E-4	9.495092746166926E-5
17	3.79803709846677E-5	1.899018549233385E-5	0.0	7.975877906780217E-4	9.495092746166926E-5
18	3.79803709846677E-5	1.899018549233385E-5	0.0	9.115289036320249E-4	1.3293129844633696E-4
19	7.59607419693354E-5	1.899018549233385E-5	0.0	0.0013483031699557035	1.3293129844633696E-4
20	7.59607419693354E-5	1.899018549233385E-5	0.0	0.0019939694766950545	1.3293129844633696E-4
21	7.59607419693354E-5	1.899018549233385E-5	0.0	0.00305741986426575	1.519214839386708E-4
22	7.59607419693354E-5	1.899018549233385E-5	0.0	0.005203310824899475	1.519214839386708E-4
23	7.59607419693354E-5	1.899018549233385E-5	0.0	0.006190800470500836	2.2788222590800622E-4
24	7.59607419693354E-5	1.899018549233385E-5	0.0	0.008108809205226554	2.2788222590800622E-4
25	7.59607419693354E-5	1.899018549233385E-5	0.0	0.009153269407304916	2.2788222590800622E-4
26	7.59607419693354E-5	1.899018549233385E-5	0.0	0.01040662164979895	2.2788222590800622E-4
27	7.59607419693354E-5	1.899018549233385E-5	0.0	0.01695823564465413	2.2788222590800622E-4
28	9.495092746166926E-5	1.899018549233385E-5	0.0	0.054539812733982826	2.2788222590800622E-4
29	9.495092746166926E-5	1.899018549233385E-5	0.0	0.1443443999272296	2.2788222590800622E-4
30	9.495092746166926E-5	1.899018549233385E-5	0.0	0.2584374343651714	2.2788222590800622E-4
31	9.495092746166926E-5	1.899018549233385E-5	0.0	0.6811399732390306	2.2788222590800622E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	4180	0.0	19.29665	37
AACGCAG	19355	0.0	16.124773	5
CGCAGAG	21710	0.0	14.409719	7
TCTTATA	6750	0.0	14.16963	37
ACGCAGA	22105	0.0	14.152227	6
TAGATCG	210	1.364242E-10	14.095238	5
GAGTACT	13215	0.0	14.027242	11
AGAGTAC	22335	0.0	13.898813	10
CAGAGTA	22655	0.0	13.816817	9
AGTACTT	13550	0.0	13.721403	12
GCAGAGT	23125	0.0	13.56	8
TACTTAC	970	0.0	13.159794	31
GTACTTT	14140	0.0	13.083451	13
GGGTAAG	2225	0.0	12.970786	1
CTCTTAT	10530	0.0	12.948243	37
TAGACAG	835	0.0	12.628742	5
TACTTTT	14750	0.0	12.592543	14
TATACAG	900	0.0	12.538889	5
GTAAGAG	2305	0.0	12.520608	3
TATACTG	630	0.0	12.333334	5
>>END_MODULE