FastQCFastQC Report
Thu 9 Feb 2017
SRR2727378.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727378.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8050869
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT368310.45747856535735454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT134940.16760923572349767No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114670.14243182940872595No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT103580.12865691889906544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC43500.018.1172437
TCTTATA74800.015.97727437
AACGCAG188550.014.9040045
ACGCAGA211850.013.2386126
GAGTACT110900.013.19522111
CGCAGAG214300.013.07862957
AGAGTAC213150.013.01900110
CTCTTAT117100.012.89154637
CAGAGTA220500.012.8367359
TCGTTAC6850.012.69343123
AGTACTT116250.012.61978512
TTATACT12350.012.5829954
GCAGAGT228650.012.4600938
GTACTTT120300.012.2718213
ACCGTAT3051.8189894E-1212.1311478
GTATTAC5650.012.1150451
TAATACT11000.012.1090924
TCTATAC9100.011.9945063
GGTATCA129400.011.937791
CAACGCA235150.011.9268554
Produced by FastQC (version 0.11.5)