##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727378.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8050869
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07908512733222	33.0	31.0	34.0	30.0	34.0
2	32.228128044314225	33.0	31.0	34.0	30.0	34.0
3	32.309383247945036	34.0	31.0	34.0	30.0	34.0
4	35.904232077307434	37.0	35.0	37.0	35.0	37.0
5	35.781084253140875	37.0	35.0	37.0	35.0	37.0
6	35.79538544224232	37.0	35.0	37.0	35.0	37.0
7	35.74739099592851	37.0	35.0	37.0	35.0	37.0
8	35.71859758244731	37.0	35.0	37.0	33.0	37.0
9	37.43602597433892	39.0	37.0	39.0	35.0	39.0
10	37.27703531134341	39.0	37.0	39.0	34.0	39.0
11	37.37653761351725	39.0	37.0	39.0	34.0	39.0
12	37.28464219204163	39.0	37.0	39.0	34.0	39.0
13	37.34140798962199	39.0	37.0	39.0	34.0	39.0
14	38.50163913982453	40.0	38.0	41.0	34.0	41.0
15	38.4634081115964	40.0	38.0	41.0	34.0	41.0
16	38.39942135439044	40.0	38.0	41.0	34.0	41.0
17	38.359530753760865	40.0	38.0	41.0	34.0	41.0
18	38.313461938083954	40.0	38.0	41.0	34.0	41.0
19	38.320056629911626	40.0	38.0	41.0	34.0	41.0
20	38.20446028869679	40.0	38.0	41.0	34.0	41.0
21	38.12569313946109	40.0	38.0	41.0	33.0	41.0
22	38.050715022192016	40.0	38.0	41.0	33.0	41.0
23	37.941574505808006	40.0	37.0	41.0	33.0	41.0
24	37.83639207146458	40.0	37.0	41.0	33.0	41.0
25	37.75345866390324	40.0	37.0	41.0	32.0	41.0
26	37.401692165156334	39.0	37.0	41.0	32.0	41.0
27	37.1856410780004	39.0	36.0	41.0	31.0	41.0
28	37.04646902092184	39.0	36.0	40.0	31.0	41.0
29	37.00754005561387	39.0	36.0	40.0	31.0	41.0
30	36.930577804706544	39.0	36.0	40.0	30.0	41.0
31	36.72509762610719	39.0	36.0	40.0	30.0	41.0
32	36.623509213725875	39.0	36.0	40.0	30.0	41.0
33	36.448246270061034	39.0	35.0	40.0	30.0	41.0
34	36.31638758499238	39.0	35.0	40.0	30.0	41.0
35	36.16485400520118	39.0	35.0	40.0	29.0	41.0
36	36.018843307474015	38.0	35.0	40.0	28.0	41.0
37	35.787915813808425	38.0	35.0	40.0	27.0	41.0
38	35.680642549270146	38.0	35.0	40.0	27.0	41.0
39	35.51692059577668	38.0	35.0	40.0	26.0	41.0
40	35.3888947888731	38.0	34.0	40.0	26.0	41.0
41	35.25847495469122	38.0	34.0	40.0	25.0	41.0
42	35.06400489189428	38.0	34.0	40.0	24.0	41.0
43	31.690587314238996	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	9.0
10	25.0
11	28.0
12	37.0
13	24.0
14	38.0
15	92.0
16	179.0
17	351.0
18	799.0
19	1649.0
20	3522.0
21	6734.0
22	11836.0
23	19428.0
24	29725.0
25	42772.0
26	58758.0
27	78453.0
28	101977.0
29	130515.0
30	165464.0
31	207016.0
32	258279.0
33	324208.0
34	418390.0
35	553023.0
36	775641.0
37	1223788.0
38	2185859.0
39	1452247.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.41696144850947	18.75037340689558	12.584827799334459	26.247837345260493
2	18.806889045145315	21.32898945443032	34.29272045042591	25.571401049998453
3	18.62739289386028	22.10141538758114	30.838149273078468	28.433042445480112
4	14.805656383180498	15.652124012948168	33.55665084104585	35.98556876282547
5	14.581208562702983	37.02538198050422	34.022898646096465	14.370510810696334
6	36.3296310000821	35.394415186733255	13.958865806908547	14.317088006276094
7	30.285078542452993	30.976531850164253	19.954032291420965	18.784357315961792
8	28.2679795187327	34.92726064726677	18.544731009782918	18.26002882421761
9	27.122401320900885	13.496195255443855	17.99664110793506	41.3847623157202
10	15.600564361437256	27.7612640324914	33.56761860117212	23.07055300489922
11	37.57514871997048	20.98936152109791	20.86676605966387	20.568723699267743
12	21.82327150025668	25.06022641779415	29.741857680208184	23.37464440174098
13	32.14077884014757	19.47102853120576	23.317482870482678	25.070709758163996
14	22.54641082844597	20.02340368474509	25.475051202547206	31.95513428426174
15	25.87677181183795	27.97891755535955	21.32405830973029	24.820252323072207
16	25.189032388925963	26.07062666154424	23.52980777603014	25.210533173499655
17	24.607492681845898	26.17065313073657	24.811098031777686	24.410756155639845
18	24.640569856496235	25.422485448465252	25.262900191271275	24.674044503767234
19	25.62501016970963	25.375844520634978	25.38001798315188	23.619127326503513
20	25.341438296909313	25.253621689782808	25.025584194699974	24.379355818607902
21	25.0207772601939	25.449364534437212	25.09065791531324	24.439200290055645
22	25.52422601833417	25.0829693043074	24.616448236830085	24.776356440528346
23	24.977030429882788	25.129622653157067	24.756557335611845	25.1367895813483
24	24.87935153335621	25.008915186671153	24.923185310803095	25.18854796916954
25	25.144254614005025	24.701668850902927	24.979390423567942	25.174686111524107
26	24.753551448918124	25.029012396053147	25.196422895466313	25.021013259562412
27	25.08131730872779	25.36872727652133	24.93527096267496	24.61468445207592
28	24.92402994012199	25.470604477603597	24.83466070557104	24.770704876703373
29	24.808042461006384	25.68519000868105	25.030341445128467	24.476426085184098
30	25.036291113418933	25.569898106651596	24.748558199121113	24.645252580808357
31	24.968025190821017	25.03612964016679	24.98050831531354	25.01533685369865
32	24.444827011841827	25.44976200705787	25.021336206066696	25.084074775033606
33	24.61760339163387	25.079093946255988	25.01293959695531	25.290363065154832
34	24.83128218829545	24.84976466515602	25.533492098803244	24.78546104774528
35	24.637787548151636	24.961553839715936	25.439763086444454	24.960895525687974
36	24.498982656406408	24.714648816171273	25.695909348419406	25.09045917900291
37	24.213535209677364	24.36698448328994	26.048479983961982	25.371000323070714
38	23.895768270481113	24.408321635838316	26.351242331728415	25.344667761952156
39	23.651831870571986	24.142114348152976	27.009519096634165	25.196534684640877
40	23.894762167910073	23.936298056768777	27.198889958338658	24.97004981698249
41	23.524975999485275	23.85336291026472	27.53557411007433	25.086086980175683
42	23.04609601770939	23.970766385591418	27.59405226938856	25.389085327310628
43	23.106201330564442	23.15938813561617	27.575843054954692	26.158567478864704
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	469.0
1	593.0
2	717.0
3	1785.5
4	2854.0
5	2854.0
6	3407.0
7	3960.0
8	4265.0
9	4570.0
10	6851.0
11	9132.0
12	9132.0
13	17372.0
14	25612.0
15	41983.0
16	58354.0
17	45584.0
18	32814.0
19	32814.0
20	39484.0
21	46154.0
22	43395.5
23	40637.0
24	50404.0
25	60171.0
26	60171.0
27	74675.0
28	89179.0
29	103837.0
30	118495.0
31	139679.5
32	160864.0
33	160864.0
34	186492.5
35	212121.0
36	245310.0
37	278499.0
38	318224.0
39	357949.0
40	357949.0
41	401280.0
42	444611.0
43	488191.0
44	531771.0
45	572978.0
46	614185.0
47	614185.0
48	654631.0
49	695077.0
50	714455.0
51	733833.0
52	731037.0
53	728241.0
54	728241.0
55	696837.0
56	665433.0
57	623178.0
58	580923.0
59	531163.0
60	481403.0
61	481403.0
62	420585.5
63	359768.0
64	306189.5
65	252611.0
66	209473.5
67	166336.0
68	166336.0
69	136461.5
70	106587.0
71	87768.5
72	68950.0
73	56390.0
74	43830.0
75	43830.0
76	36558.5
77	29287.0
78	23937.0
79	18587.0
80	15059.5
81	11532.0
82	11532.0
83	9140.5
84	6749.0
85	5330.5
86	3912.0
87	3258.0
88	2604.0
89	2604.0
90	1939.5
91	1275.0
92	889.5
93	504.0
94	356.5
95	209.0
96	209.0
97	138.5
98	68.0
99	50.0
100	32.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	8050869.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.80821728505987
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.6605376954853	45.015208140394066
2	9.96199920350237	11.119228322853072
3	3.4153859228071717	5.71819799077072
4	1.6725928729777775	3.7337770593834536
5	0.979604836246187	2.7334999777361344
6	0.630528009374342	2.1113186490887728
7	0.4382075183100915	1.711890627845751
8	0.3340679282804194	1.4914988423554756
9	0.24336496312424527	1.2223588267447618
>10	1.5093287309931287	15.717120014816613
>50	0.11254404257277123	4.2609269685504545
>100	0.04063694214266601	3.7104606899922703
>500	8.232189023929633E-4	0.30617127182337883
>1k	2.891469910438441E-4	0.2527515824553066
>5k	0.0	0.0
>10k+	8.89682900798052E-5	0.8955910351898161
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	36831	0.45747856535735454	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13494	0.16760923572349767	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11467	0.14243182940872595	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10358	0.12865691889906544	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2421019395545996E-5
2	0.0	0.0	0.0	0.0	1.2421019395545996E-5
3	0.0	0.0	0.0	0.0	1.2421019395545996E-5
4	0.0	0.0	0.0	1.2421019395545996E-5	1.2421019395545996E-5
5	0.0	1.2421019395545996E-5	0.0	1.2421019395545996E-5	1.2421019395545996E-5
6	0.0	1.2421019395545996E-5	0.0	1.2421019395545996E-5	6.210509697772998E-5
7	0.0	1.2421019395545996E-5	0.0	1.2421019395545996E-5	6.210509697772998E-5
8	1.2421019395545996E-5	1.2421019395545996E-5	0.0	1.2421019395545996E-5	9.936815516436797E-5
9	1.2421019395545996E-5	1.2421019395545996E-5	0.0	1.2421019395545996E-5	1.6147325214209795E-4
10	3.726305818663799E-5	1.2421019395545996E-5	0.0	1.2421019395545996E-5	1.6147325214209795E-4
11	6.210509697772998E-5	1.2421019395545996E-5	0.0	3.726305818663799E-5	1.6147325214209795E-4
12	6.210509697772998E-5	1.2421019395545996E-5	0.0	4.9684077582183984E-5	1.6147325214209795E-4
13	6.210509697772998E-5	1.2421019395545996E-5	0.0	4.9684077582183984E-5	1.6147325214209795E-4
14	6.210509697772998E-5	1.2421019395545996E-5	0.0	4.9684077582183984E-5	2.484203879109199E-4
15	6.210509697772998E-5	1.2421019395545996E-5	0.0	6.210509697772998E-5	2.981044654931039E-4
16	7.452611637327598E-5	1.2421019395545996E-5	0.0	1.2421019395545996E-4	3.726305818663799E-4
17	7.452611637327598E-5	1.2421019395545996E-5	0.0	2.2357834911982793E-4	3.726305818663799E-4
18	7.452611637327598E-5	1.2421019395545996E-5	0.0	2.8568344609755794E-4	3.974726206574719E-4
19	7.452611637327598E-5	1.2421019395545996E-5	0.0	4.347356788441099E-4	3.974726206574719E-4
20	9.936815516436797E-5	1.2421019395545996E-5	0.0	7.079981055461218E-4	3.974726206574719E-4
21	9.936815516436797E-5	1.2421019395545996E-5	0.0	0.0012296809201590537	4.2231465944856387E-4
22	9.936815516436797E-5	1.2421019395545996E-5	0.0	0.0019997841226829056	4.2231465944856387E-4
23	9.936815516436797E-5	1.2421019395545996E-5	0.0	0.0023475726657581934	5.216828146129319E-4
24	9.936815516436797E-5	1.2421019395545996E-5	0.0	0.003092833829490953	5.216828146129319E-4
25	9.936815516436797E-5	1.2421019395545996E-5	0.0	0.003564832566521701	5.341038340084778E-4
26	9.936815516436797E-5	1.2421019395545996E-5	0.0	0.004397040866023283	5.341038340084778E-4
27	9.936815516436797E-5	1.2421019395545996E-5	0.0	0.008297240956224725	5.465248534040238E-4
28	9.936815516436797E-5	1.2421019395545996E-5	0.0	0.031710862516828926	5.465248534040238E-4
29	9.936815516436797E-5	1.2421019395545996E-5	0.0	0.08735702940887499	5.465248534040238E-4
30	9.936815516436797E-5	1.2421019395545996E-5	0.0	0.16015662408617007	5.465248534040238E-4
31	9.936815516436797E-5	1.2421019395545996E-5	0.0	0.4438527070804406	5.589458727995699E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	4350	0.0	18.11724	37
TCTTATA	7480	0.0	15.977274	37
AACGCAG	18855	0.0	14.904004	5
ACGCAGA	21185	0.0	13.238612	6
GAGTACT	11090	0.0	13.195221	11
CGCAGAG	21430	0.0	13.0786295	7
AGAGTAC	21315	0.0	13.019001	10
CTCTTAT	11710	0.0	12.891546	37
CAGAGTA	22050	0.0	12.836735	9
TCGTTAC	685	0.0	12.693431	23
AGTACTT	11625	0.0	12.619785	12
TTATACT	1235	0.0	12.582995	4
GCAGAGT	22865	0.0	12.460093	8
GTACTTT	12030	0.0	12.27182	13
ACCGTAT	305	1.8189894E-12	12.131147	8
GTATTAC	565	0.0	12.115045	1
TAATACT	1100	0.0	12.109092	4
TCTATAC	910	0.0	11.994506	3
GGTATCA	12940	0.0	11.93779	1
CAACGCA	23515	0.0	11.926855	4
>>END_MODULE