FastQCFastQC Report
Thu 9 Feb 2017
SRR2727377.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727377.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1329569
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAA905.456968E-1226.72222320
CGACTAT451.3233644E-424.66666836
ATTACGG851.9772415E-923.94117525
CGTGTTA400.001931345223.12500235
TATACCG652.6833713E-622.769235
CATACCG450.003826118320.5555575
TTACGGG656.903171E-519.92307726
ATACCGA759.2675655E-619.7333346
CGCGCAT609.237854E-418.510
CATTACG1103.8529834E-818.524
TATATGA650.001580110217.0769232
GGGGTAA1201.04153514E-716.95833436
TCGTTAC904.446799E-516.44444523
TTTATGC904.446799E-516.4444453
TATATAC803.3830633E-416.18753
TACCGAC803.3830633E-416.18757
ACCCGTT1151.2424771E-616.0869567
TTATGCT1403.473906E-815.8571424
TTCTAGG1302.5896588E-715.6538462
TACCCGT957.061008E-515.5789486
Produced by FastQC (version 0.11.5)