##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727377.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1329569
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06241045030382	33.0	31.0	34.0	30.0	34.0
2	32.22111300729785	33.0	31.0	34.0	30.0	34.0
3	32.28909142737233	34.0	31.0	34.0	30.0	34.0
4	35.89426874423215	37.0	35.0	37.0	35.0	37.0
5	35.765219405687105	37.0	35.0	37.0	35.0	37.0
6	35.77706835824241	37.0	35.0	37.0	35.0	37.0
7	35.72439339364862	37.0	35.0	37.0	35.0	37.0
8	35.67933292668526	37.0	35.0	37.0	33.0	37.0
9	37.41389277277072	39.0	37.0	39.0	35.0	39.0
10	37.23692414609546	39.0	37.0	39.0	34.0	39.0
11	37.33941149349902	39.0	37.0	39.0	34.0	39.0
12	37.22800471430968	39.0	37.0	39.0	34.0	39.0
13	37.282657763530885	39.0	37.0	39.0	34.0	39.0
14	38.44264946008819	40.0	38.0	41.0	34.0	41.0
15	38.386330457464034	40.0	38.0	41.0	34.0	41.0
16	38.30140444008547	40.0	38.0	41.0	34.0	41.0
17	38.24333675048079	40.0	38.0	41.0	33.0	41.0
18	38.17735897873672	40.0	38.0	41.0	33.0	41.0
19	38.153303062872254	40.0	38.0	41.0	33.0	41.0
20	38.013584853437465	40.0	37.0	41.0	33.0	41.0
21	37.91869094420824	40.0	37.0	41.0	33.0	41.0
22	37.8349186841751	40.0	37.0	41.0	33.0	41.0
23	37.701217462200155	40.0	37.0	41.0	32.0	41.0
24	37.5551761510685	39.0	37.0	41.0	32.0	41.0
25	37.46012880865905	39.0	37.0	41.0	32.0	41.0
26	37.04833145177121	39.0	36.0	40.0	31.0	41.0
27	36.79801950857759	39.0	36.0	40.0	30.0	41.0
28	36.630826230154284	39.0	36.0	40.0	30.0	41.0
29	36.58866670327001	39.0	36.0	40.0	30.0	41.0
30	36.488434221917025	39.0	36.0	40.0	30.0	41.0
31	36.26097028435531	39.0	35.0	40.0	29.0	41.0
32	36.15546616986407	39.0	35.0	40.0	29.0	41.0
33	35.95591879774574	39.0	35.0	40.0	27.0	41.0
34	35.786032165310715	38.0	35.0	40.0	27.0	41.0
35	35.625937427843155	38.0	35.0	40.0	26.0	41.0
36	35.462511535693146	38.0	34.0	40.0	26.0	41.0
37	35.19357852055816	38.0	34.0	40.0	25.0	41.0
38	35.08220934754044	38.0	34.0	40.0	24.0	41.0
39	34.89550222666142	38.0	33.0	40.0	24.0	41.0
40	34.76340001910393	38.0	33.0	40.0	24.0	41.0
41	34.609642673678465	38.0	33.0	40.0	23.0	41.0
42	34.40558180884181	38.0	33.0	40.0	23.0	41.0
43	30.78138103400425	35.0	27.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	1.0
12	7.0
13	5.0
14	10.0
15	25.0
16	46.0
17	90.0
18	210.0
19	449.0
20	888.0
21	1659.0
22	2756.0
23	4552.0
24	6823.0
25	9301.0
26	12556.0
27	15965.0
28	19945.0
29	25117.0
30	30813.0
31	38000.0
32	46709.0
33	57518.0
34	72357.0
35	94502.0
36	129337.0
37	195489.0
38	345243.0
39	219191.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.049890603646745	17.356752451358297	11.72688292221013	27.866474022784825
2	20.772295382939884	19.90968501822771	31.854834160543753	27.46318543828865
3	19.601540047940347	20.43143304333961	29.22097311233941	30.74605379638063
4	16.020229111840003	15.004185566901757	30.883090685778626	38.09249463547962
5	16.023688879629415	35.513162536130125	32.750688380971575	15.71246020326888
6	39.049195641595134	32.961508579095934	12.940885354577311	15.048410424731623
7	32.11732523847954	29.510841483217494	18.32142596585811	20.05040731244486
8	29.708198671900444	32.991894365768154	17.362242952415407	19.937664009915995
9	27.927471233159018	12.700431493213213	16.228040816234433	43.14405645739334
10	16.9484246398645	26.205935908553823	31.620322074296258	25.22531737728542
11	39.99672074183439	19.425844014112844	18.88942958206757	21.688005661985198
12	23.447448007587422	23.493854023371483	27.509817091102455	25.54888087793864
13	34.08698608346013	18.049232495643324	21.278324028312934	26.58545739258361
14	23.980402671843283	18.67612737661603	22.970526539051377	34.372943412489306
15	27.925515712234567	25.983457797226016	19.39275058308369	26.698275907455727
16	27.496278869317802	24.2707975291241	21.33871953994114	26.89420406161696
17	27.205507950320744	24.311487406821307	22.0631648301066	26.41983981275135
18	27.067192451087536	23.42563642804548	22.52098236345763	26.98618875740936
19	28.357309774821765	23.501676107069283	22.29519490902691	25.845819209082038
20	27.647305254559935	23.135993694197143	22.33107119675624	26.88562985448668
21	27.31035395680856	23.408638438471417	22.5379803530317	26.74302725168833
22	28.036829980241716	23.112076169044254	21.744038857705018	27.10705499300901
23	27.38263301866996	23.03024513959035	22.07835772344271	27.50876411829698
24	27.146240623841262	23.249113058442248	22.097461658627722	27.507184659088775
25	27.424902355575377	22.68990928639281	22.33979582857302	27.545392529458795
26	27.170458998367142	22.892456126759875	22.459233029650964	27.477851845222023
27	27.582923488739585	23.309207720697458	22.192605272836534	26.915263517726423
28	27.290272261161324	23.198419939093046	22.166807439102445	27.34450036064319
29	27.2775613751524	23.53807888120135	22.15379570372053	27.03056403992572
30	27.34231920268899	23.496787304758158	22.12829871935943	27.03259477319342
31	27.18459891889778	22.626730918064425	22.348595672733044	27.84007449030475
32	26.90661409825289	23.292510580496387	22.40237249815542	27.398502823095306
33	27.06328140923863	22.809421699814	22.53279070134758	27.594506189599787
34	27.173768341470055	22.7546671139294	22.80513459624886	27.266429948351682
35	27.113748891558092	23.016030006716463	22.72232580633273	27.147895295392715
36	26.809214113746638	22.515792711773514	22.988051015028176	27.68694215945167
37	26.580493377929237	22.29406672387819	23.285816681947306	27.83962321624526
38	26.276485086520516	22.082419189978104	23.745965797939032	27.895129925562344
39	25.93825517893393	21.872952814032217	24.37052909627105	27.818262910762808
40	26.29792060434622	21.41724122629213	24.940262596375216	27.344575572986436
41	25.881770709154623	21.317434446801933	25.193051282032	27.60774356201145
42	25.195382864672688	21.346992897698428	25.56836087483989	27.889263362788995
43	25.594083496230734	20.238137321192056	25.3141431546614	28.85363602791581
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	23.5
2	27.0
3	63.5
4	100.0
5	100.0
6	115.5
7	131.0
8	135.0
9	139.0
10	192.0
11	245.0
12	245.0
13	505.5
14	766.0
15	1275.0
16	1784.0
17	1376.5
18	969.0
19	969.0
20	1239.0
21	1509.0
22	1478.5
23	1448.0
24	1933.5
25	2419.0
26	2419.0
27	3164.0
28	3909.0
29	4926.0
30	5943.0
31	7772.5
32	9602.0
33	9602.0
34	12133.0
35	14664.0
36	18168.0
37	21672.0
38	27272.0
39	32872.0
40	32872.0
41	41210.0
42	49548.0
43	58524.0
44	67500.0
45	78174.0
46	88848.0
47	88848.0
48	100830.5
49	112813.0
50	121459.0
51	130105.0
52	134204.0
53	138303.0
54	138303.0
55	137325.0
56	136347.0
57	131651.5
58	126956.0
59	118380.5
60	109805.0
61	109805.0
62	97927.5
63	86050.0
64	74954.0
65	63858.0
66	52989.0
67	42120.0
68	42120.0
69	34646.0
70	27172.0
71	22456.0
72	17740.0
73	14872.5
74	12005.0
75	12005.0
76	10079.0
77	8153.0
78	6803.0
79	5453.0
80	4406.5
81	3360.0
82	3360.0
83	2709.5
84	2059.0
85	1674.5
86	1290.0
87	1123.0
88	956.0
89	956.0
90	766.5
91	577.0
92	396.5
93	216.0
94	148.0
95	80.0
96	80.0
97	52.5
98	25.0
99	18.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1329569.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.09954924512064
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.1212799315493	57.11599514574692
2	8.097634903963442	10.866953040150056
3	2.5352765477468315	5.103477406966141
4	1.22355224899234	3.2839921754095864
5	0.7329903591402285	2.4591661349664213
6	0.5064744377075386	2.0390523884611067
7	0.3258822538590861	1.530658663865006
8	0.25815326888462536	1.3857574382650233
9	0.18755818839682087	1.1326562908792288
>10	0.9552845032182254	11.074183389686059
>50	0.03860108951947261	1.7433709415654493
>100	0.016975021386442666	2.088683129604761
>500	2.2483042372627924E-4	0.09066698073262312
>1k	1.1241521186313962E-4	0.08538687370160514
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	1.5042468649615026E-4	0.0	0.0	0.0	0.0
12	1.5042468649615026E-4	0.0	0.0	0.0	0.0
13	1.5042468649615026E-4	0.0	0.0	0.0	0.0
14	1.5042468649615026E-4	0.0	0.0	0.0	7.521234324807513E-5
15	1.5042468649615026E-4	0.0	0.0	0.0	7.521234324807513E-5
16	1.5042468649615026E-4	0.0	0.0	1.5042468649615026E-4	7.521234324807513E-5
17	1.5042468649615026E-4	0.0	0.0	1.5042468649615026E-4	7.521234324807513E-5
18	1.5042468649615026E-4	0.0	0.0	2.256370297442254E-4	1.5042468649615026E-4
19	1.5042468649615026E-4	0.0	0.0	2.256370297442254E-4	1.5042468649615026E-4
20	1.5042468649615026E-4	0.0	0.0	4.512740594884508E-4	1.5042468649615026E-4
21	1.5042468649615026E-4	0.0	0.0	7.521234324807512E-4	1.5042468649615026E-4
22	1.5042468649615026E-4	0.0	0.0	0.0015794592082095777	1.5042468649615026E-4
23	1.5042468649615026E-4	0.0	0.0	0.001880308581201878	1.5042468649615026E-4
24	1.5042468649615026E-4	0.0	0.0	0.002406794983938404	1.5042468649615026E-4
25	1.5042468649615026E-4	0.0	0.0	0.0026324320136826293	2.256370297442254E-4
26	1.5042468649615026E-4	0.0	0.0	0.003459767789411456	2.256370297442254E-4
27	1.5042468649615026E-4	0.0	0.0	0.006769110892326761	2.256370297442254E-4
28	1.5042468649615026E-4	0.0	0.0	0.0287311151207647	3.008493729923005E-4
29	1.5042468649615026E-4	0.0	0.0	0.08077805664843268	3.008493729923005E-4
30	1.5042468649615026E-4	0.0	0.0	0.15388445428556172	3.008493729923005E-4
31	1.5042468649615026E-4	0.0	0.0	0.3628995561719625	3.008493729923005E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATCAA	90	5.456968E-12	26.722223	20
CGACTAT	45	1.3233644E-4	24.666668	36
ATTACGG	85	1.9772415E-9	23.941175	25
CGTGTTA	40	0.0019313452	23.125002	35
TATACCG	65	2.6833713E-6	22.76923	5
CATACCG	45	0.0038261183	20.555557	5
TTACGGG	65	6.903171E-5	19.923077	26
ATACCGA	75	9.2675655E-6	19.733334	6
CGCGCAT	60	9.237854E-4	18.5	10
CATTACG	110	3.8529834E-8	18.5	24
TATATGA	65	0.0015801102	17.076923	2
GGGGTAA	120	1.04153514E-7	16.958334	36
TCGTTAC	90	4.446799E-5	16.444445	23
TTTATGC	90	4.446799E-5	16.444445	3
TATATAC	80	3.3830633E-4	16.1875	3
TACCGAC	80	3.3830633E-4	16.1875	7
ACCCGTT	115	1.2424771E-6	16.086956	7
TTATGCT	140	3.473906E-8	15.857142	4
TTCTAGG	130	2.5896588E-7	15.653846	2
TACCCGT	95	7.061008E-5	15.578948	6
>>END_MODULE