FastQCFastQC Report
Thu 9 Feb 2017
SRR2727376.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727376.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4832297
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT381490.7894589260552486No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT152920.31645405901168744No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122140.25275764300083375No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT105720.21877794349147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC27150.018.12522937
AACGCAG187200.014.16159255
GTACTAT3550.014.0704231
TCGTTAC4250.013.92941223
TCTTATA44400.013.70833337
TAATACC3950.013.5822784
TAGTACT4700.013.3829794
TAGACAG10200.012.8774515
ATACCCG2753.6379788E-1212.7818175
TCTATAC4350.012.758623
CGCAGAG207300.012.7438497
CTCTTAT67500.012.63481537
GAGTACT115750.012.56241911
ACGCAGA210600.012.5353756
AGAGTAC209500.012.3804310
AGTACTT118350.012.28643912
CGTGAAA8300.012.25903610
CAGAGTA215400.012.2045039
GGTATCA123500.012.1635621
ACCGCTA7000.012.1571438
Produced by FastQC (version 0.11.5)