##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727376.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4832297
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46624410709855	31.0	31.0	34.0	30.0	34.0
2	31.635761626406655	31.0	31.0	34.0	30.0	34.0
3	31.69620120617586	31.0	31.0	34.0	30.0	34.0
4	35.44016830919126	37.0	35.0	37.0	33.0	37.0
5	35.1084674638169	37.0	35.0	37.0	32.0	37.0
6	35.121055473204564	37.0	35.0	37.0	32.0	37.0
7	35.077031896011356	36.0	35.0	37.0	32.0	37.0
8	35.023985487646975	36.0	35.0	37.0	32.0	37.0
9	36.64319887622801	38.0	35.0	39.0	32.0	39.0
10	36.36155993723068	38.0	35.0	39.0	32.0	39.0
11	36.52228743390565	38.0	35.0	39.0	32.0	39.0
12	36.39865761562255	38.0	35.0	39.0	32.0	39.0
13	36.492401439729385	38.0	35.0	39.0	32.0	39.0
14	37.422097606997255	39.0	36.0	40.0	32.0	41.0
15	37.365190094896896	39.0	36.0	40.0	32.0	41.0
16	37.27682652783966	39.0	36.0	40.0	32.0	41.0
17	37.23089040263874	39.0	36.0	40.0	32.0	41.0
18	37.17179345557609	39.0	36.0	40.0	32.0	41.0
19	37.18184002349194	39.0	36.0	40.0	32.0	41.0
20	37.04370406040854	39.0	36.0	40.0	31.0	41.0
21	36.942272174082014	39.0	36.0	40.0	31.0	41.0
22	36.85570526811576	39.0	36.0	40.0	31.0	41.0
23	36.72100742152231	38.0	36.0	40.0	31.0	41.0
24	36.58445228014752	38.0	36.0	40.0	30.0	41.0
25	36.492768139044436	38.0	35.0	40.0	30.0	41.0
26	36.08172821331139	38.0	35.0	40.0	29.0	41.0
27	35.805468082777196	38.0	34.0	40.0	28.0	41.0
28	35.63989196028307	38.0	34.0	40.0	27.0	41.0
29	35.55370996443306	38.0	34.0	40.0	27.0	41.0
30	35.45164856381965	38.0	34.0	40.0	27.0	41.0
31	35.19299227675782	38.0	34.0	40.0	26.0	41.0
32	35.07011530955155	38.0	34.0	40.0	26.0	41.0
33	34.85826988697094	38.0	33.0	40.0	25.0	41.0
34	34.678817961727106	38.0	33.0	40.0	25.0	40.0
35	34.50096134405646	38.0	33.0	40.0	24.0	40.0
36	34.31978063434429	37.0	33.0	40.0	24.0	40.0
37	34.01987129516253	37.0	33.0	39.0	23.0	40.0
38	33.88104642574742	37.0	32.0	39.0	23.0	40.0
39	33.66228027788855	37.0	32.0	39.0	22.0	40.0
40	33.497756656927336	37.0	31.0	39.0	21.0	40.0
41	33.31164309644047	37.0	31.0	39.0	20.0	40.0
42	33.058744319730344	37.0	31.0	39.0	19.0	40.0
43	30.040680239645866	33.0	25.0	38.0	10.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	6.0
10	17.0
11	33.0
12	41.0
13	49.0
14	113.0
15	222.0
16	383.0
17	702.0
18	1399.0
19	2662.0
20	5047.0
21	8797.0
22	14335.0
23	21731.0
24	31543.0
25	43331.0
26	58493.0
27	75840.0
28	97016.0
29	120940.0
30	149612.0
31	182068.0
32	225963.0
33	279969.0
34	357066.0
35	471253.0
36	661345.0
37	916836.0
38	918305.0
39	187177.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.1523132787575	20.084361536552905	13.669027379732663	25.094297804956938
2	19.166412991585574	22.859749721509253	33.739565262648384	24.234272024256786
3	19.203517499027896	23.10367098711027	31.295986153168982	26.396825360692855
4	15.2511528161452	16.65733708006772	33.32450385396427	34.767006249822806
5	14.427941825595573	37.775823795598654	33.644744931861595	14.151489446944176
6	35.327671291727306	36.16255375031791	13.961890173555144	14.547884784399635
7	29.24056613242108	31.613412834517412	20.250431627029545	18.895589406031952
8	28.206834141196207	35.171575753725406	18.528331350494394	18.093258754584
9	26.960697986899397	13.76991107955492	18.510741372063844	40.75864956148184
10	15.587411121460457	28.69883618494476	33.355089722341155	22.35866297125363
11	37.10744600342239	21.513247219696968	20.89979154840855	20.479515228472092
12	21.433388717622282	26.130554475438906	29.829789021659884	22.606267785278927
13	32.0928742583496	20.107559613988958	23.631825610056666	24.167740517604773
14	22.052328323362573	21.001565094198472	26.36350787213617	30.582598710302783
15	25.494935431328	28.871693937686366	21.947243722809258	23.68612690817638
16	24.298506486666692	26.686563346582382	24.327022945816452	24.687907220934473
17	24.268810464257474	26.843424565998326	25.394631166089336	23.493133803654867
18	24.012389966924633	25.751169681830397	26.123311543143974	24.113128808100992
19	25.191456568170373	25.800918279650443	26.45925943707516	22.54836571510402
20	24.556975699134387	25.745892688301236	26.075942765935125	23.621188846629256
21	24.212977803309688	26.04817129410713	26.345359153214297	23.393491749368884
22	24.686624187213656	25.82028795001632	25.542490455367293	23.950597407402732
23	24.13022212831703	25.740160424742104	25.815755943808917	24.313861503131946
24	23.9974488323048	25.876472410532713	25.932263683295957	24.193815073866528
25	24.23685878579069	25.548864235786834	26.04719867177038	24.167078306652094
26	23.902359478318488	25.846238341724444	26.206005135859822	24.045397044097246
27	24.315806747805443	26.19189176493084	25.903871388699823	23.588430098563894
28	23.971125946935796	26.266721602583615	25.951654047754104	23.810498402726488
29	23.97458186034509	26.37544836337667	26.0212896682468	23.62868010803144
30	24.038754240478184	26.44733963992693	25.814886791933528	23.699019327661357
31	24.016590867655694	25.638407573044454	26.1266019038151	24.218399655484752
32	23.6223063276119	26.285118650612745	25.850935900670013	24.241639121105347
33	23.856170264369098	25.728219933501606	26.018392495328825	24.397217306800474
34	24.013942023844976	25.767042050602434	26.371226768553342	23.847789156999248
35	23.883507160259395	25.888661230880473	26.257202320138852	23.970629288721284
36	23.654278700170952	25.54294572539726	26.531585289563118	24.271190284868666
37	23.451787007296943	25.398480267251784	26.721412197967137	24.428320527484136
38	23.185143628382114	25.29629283961644	27.142681834332617	24.37588169766883
39	22.991881500661073	25.165940752399944	27.579451345809247	24.26272640112973
40	23.2227034058544	24.903829379692517	27.803485588737615	24.06998162571547
41	22.85670768994538	24.768241687131397	28.061685778005778	24.313364844917437
42	22.425111701536558	24.986274643301105	27.968376115954797	24.620237539207544
43	22.718574624034904	24.207079987012385	27.78192234459099	25.292423044361716
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1283.0
1	1179.0
2	1075.0
3	1903.0
4	2731.0
5	2731.0
6	3523.0
7	4315.0
8	4960.5
9	5606.0
10	8208.0
11	10810.0
12	10810.0
13	19769.5
14	28729.0
15	46230.5
16	63732.0
17	49474.0
18	35216.0
19	35216.0
20	41110.0
21	47004.0
22	42496.5
23	37989.0
24	45170.5
25	52352.0
26	52352.0
27	62326.0
28	72300.0
29	81510.5
30	90721.0
31	103038.5
32	115356.0
33	115356.0
34	129992.5
35	144629.0
36	163472.0
37	182315.0
38	201827.5
39	221340.0
40	221340.0
41	246914.5
42	272489.0
43	294907.0
44	317325.0
45	338468.5
46	359612.0
47	359612.0
48	381115.0
49	402618.0
50	409119.5
51	415621.0
52	410870.0
53	406119.0
54	406119.0
55	384882.5
56	363646.0
57	340307.5
58	316969.0
59	288815.5
60	260662.0
61	260662.0
62	228513.5
63	196365.0
64	167036.5
65	137708.0
66	114086.5
67	90465.0
68	90465.0
69	74859.5
70	59254.0
71	49109.0
72	38964.0
73	32491.0
74	26018.0
75	26018.0
76	22053.5
77	18089.0
78	15148.5
79	12208.0
80	10112.0
81	8016.0
82	8016.0
83	6511.5
84	5007.0
85	4138.5
86	3270.0
87	2826.5
88	2383.0
89	2383.0
90	1788.5
91	1194.0
92	858.5
93	523.0
94	360.5
95	198.0
96	198.0
97	126.0
98	54.0
99	35.5
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4832297.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.60902299815151
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.60095395021902	50.6697214063623
2	8.869523819320305	10.751463462956739
3	2.794984057927398	5.0820375913916545
4	1.3541687154702342	3.2829937127725084
5	0.761740703540557	2.3084179909758866
6	0.4910853759498188	1.7858522906999068
7	0.36165355256731874	1.5343627939441071
8	0.25491699504726567	1.236021601235152
9	0.21691111554772113	1.1832093711707876
>10	1.193449112702018	13.208741076253158
>50	0.06790098498641053	2.78807717202363
>100	0.029880962896569836	3.14946732825945
>500	0.0019098393840459187	0.7893353507565827
>1k	7.843974870518057E-4	0.654668024871212
>5k	0.0	0.0
>10k+	1.364169542682873E-4	1.5756308263268337
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	38149	0.7894589260552486	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15292	0.31645405901168744	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12214	0.25275764300083375	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10572	0.21877794349147	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.06940922712325E-5
2	0.0	0.0	0.0	0.0	4.1388184542465E-5
3	0.0	0.0	0.0	0.0	4.1388184542465E-5
4	0.0	0.0	0.0	0.0	4.1388184542465E-5
5	0.0	2.06940922712325E-5	0.0	0.0	4.1388184542465E-5
6	0.0	2.06940922712325E-5	0.0	0.0	4.1388184542465E-5
7	0.0	2.06940922712325E-5	0.0	0.0	4.1388184542465E-5
8	0.0	2.06940922712325E-5	0.0	0.0	6.20822768136975E-5
9	0.0	2.06940922712325E-5	0.0	0.0	6.20822768136975E-5
10	2.06940922712325E-5	2.06940922712325E-5	0.0	0.0	8.277636908493E-5
11	2.06940922712325E-5	2.06940922712325E-5	0.0	0.0	8.277636908493E-5
12	2.06940922712325E-5	2.06940922712325E-5	0.0	2.06940922712325E-5	8.277636908493E-5
13	2.06940922712325E-5	2.06940922712325E-5	0.0	2.06940922712325E-5	8.277636908493E-5
14	4.1388184542465E-5	2.06940922712325E-5	0.0	2.06940922712325E-5	1.034704613561625E-4
15	4.1388184542465E-5	2.06940922712325E-5	0.0	6.20822768136975E-5	1.034704613561625E-4
16	4.1388184542465E-5	2.06940922712325E-5	0.0	8.277636908493E-5	1.6555273816986E-4
17	4.1388184542465E-5	2.06940922712325E-5	0.0	1.862468304410925E-4	1.6555273816986E-4
18	8.277636908493E-5	2.06940922712325E-5	0.0	1.862468304410925E-4	1.6555273816986E-4
19	8.277636908493E-5	2.06940922712325E-5	0.0	3.3110547633972E-4	1.6555273816986E-4
20	1.034704613561625E-4	2.06940922712325E-5	0.0	6.208227681369751E-4	1.862468304410925E-4
21	1.24164553627395E-4	2.06940922712325E-5	0.0	0.00115886916718902	2.06940922712325E-4
22	1.24164553627395E-4	2.06940922712325E-5	0.0	0.0022142678730218777	2.06940922712325E-4
23	1.24164553627395E-4	2.06940922712325E-5	0.0	0.00264884381071776	3.1041138406848754E-4
24	1.24164553627395E-4	2.06940922712325E-5	0.0	0.0037042425165506178	3.1041138406848754E-4
25	1.24164553627395E-4	2.06940922712325E-5	0.0	0.004014653900619105	3.1041138406848754E-4
26	1.24164553627395E-4	2.06940922712325E-5	0.0	0.005007970329638265	3.3110547633972E-4
27	1.24164553627395E-4	2.06940922712325E-5	0.0	0.008898459676629975	3.3110547633972E-4
28	1.24164553627395E-4	2.06940922712325E-5	0.0	0.032137925297224076	3.3110547633972E-4
29	1.24164553627395E-4	2.06940922712325E-5	0.0	0.08826030353680661	3.3110547633972E-4
30	1.24164553627395E-4	2.06940922712325E-5	0.0	0.15992394507208477	3.3110547633972E-4
31	1.24164553627395E-4	2.06940922712325E-5	0.0	0.41516487914546646	3.3110547633972E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2715	0.0	18.125229	37
AACGCAG	18720	0.0	14.1615925	5
GTACTAT	355	0.0	14.070423	1
TCGTTAC	425	0.0	13.929412	23
TCTTATA	4440	0.0	13.708333	37
TAATACC	395	0.0	13.582278	4
TAGTACT	470	0.0	13.382979	4
TAGACAG	1020	0.0	12.877451	5
ATACCCG	275	3.6379788E-12	12.781817	5
TCTATAC	435	0.0	12.75862	3
CGCAGAG	20730	0.0	12.743849	7
CTCTTAT	6750	0.0	12.634815	37
GAGTACT	11575	0.0	12.562419	11
ACGCAGA	21060	0.0	12.535375	6
AGAGTAC	20950	0.0	12.38043	10
AGTACTT	11835	0.0	12.286439	12
CGTGAAA	830	0.0	12.259036	10
CAGAGTA	21540	0.0	12.204503	9
GGTATCA	12350	0.0	12.163562	1
ACCGCTA	700	0.0	12.157143	8
>>END_MODULE