FastQCFastQC Report
Thu 9 Feb 2017
SRR2727375.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727375.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8616702
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT401780.46628048643204795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT154430.17922170222435452No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128030.1485835299862987No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT119630.1388350206378264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC53250.019.00375637
TCTTATA92850.015.48142137
AACGCAG182400.014.9703945
GGTATCA115350.014.1616811
TAGTACT8000.013.41254
TATACCG4150.013.3734945
ACGCAGA207650.013.1500126
CTCTTAT147850.013.07575237
CGCAGAG210500.012.9983377
AGAGTAC213750.012.77473610
CAGAGTA221900.012.455619
TAATACT11900.012.4369754
CAACGCA220500.012.3752844
TCAACGC221500.012.3361183
ATCAACG222550.012.3111662
GCAGAGT228100.012.2549318
TATCAAC222550.012.2197261
GTCTTAG13050.012.1915711
GAGTACT124550.012.12043411
TATACTG11050.011.7194585
Produced by FastQC (version 0.11.5)