##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727375.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8616702
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.061694718002315	33.0	31.0	34.0	30.0	34.0
2	32.21361896929939	33.0	31.0	34.0	30.0	34.0
3	32.297659243641014	34.0	31.0	34.0	30.0	34.0
4	35.891826478390456	37.0	35.0	37.0	35.0	37.0
5	35.76958968756259	37.0	35.0	37.0	35.0	37.0
6	35.78550134378559	37.0	35.0	37.0	35.0	37.0
7	35.737517091806126	37.0	35.0	37.0	35.0	37.0
8	35.70752000011141	37.0	35.0	37.0	33.0	37.0
9	37.431295059292985	39.0	37.0	39.0	35.0	39.0
10	37.267624666606785	39.0	37.0	39.0	34.0	39.0
11	37.37577985173446	39.0	37.0	39.0	34.0	39.0
12	37.275377516827206	39.0	37.0	39.0	34.0	39.0
13	37.33592434785374	39.0	37.0	39.0	34.0	39.0
14	38.49667158037959	40.0	38.0	41.0	34.0	41.0
15	38.45569441765539	40.0	38.0	41.0	34.0	41.0
16	38.39330210096624	40.0	38.0	41.0	34.0	41.0
17	38.353191511090905	40.0	38.0	41.0	34.0	41.0
18	38.30632938216965	40.0	38.0	41.0	34.0	41.0
19	38.313735463986106	40.0	38.0	41.0	34.0	41.0
20	38.20021488499892	40.0	38.0	41.0	34.0	41.0
21	38.12407101928325	40.0	38.0	41.0	33.0	41.0
22	38.050809810992654	40.0	38.0	41.0	33.0	41.0
23	37.942477063730415	40.0	37.0	41.0	33.0	41.0
24	37.83379998519155	40.0	37.0	41.0	33.0	41.0
25	37.758845669723755	40.0	37.0	41.0	32.0	41.0
26	37.40312012647066	39.0	37.0	41.0	32.0	41.0
27	37.19726712145784	39.0	36.0	41.0	31.0	41.0
28	37.06241854482144	39.0	36.0	40.0	31.0	41.0
29	37.030232332509584	39.0	36.0	40.0	31.0	41.0
30	36.95950805772325	39.0	36.0	40.0	31.0	41.0
31	36.75408387106807	39.0	36.0	40.0	30.0	41.0
32	36.659994044125	39.0	36.0	40.0	30.0	41.0
33	36.49053442953	39.0	35.0	40.0	30.0	41.0
34	36.362680176243764	39.0	35.0	40.0	30.0	41.0
35	36.21684050347801	39.0	35.0	40.0	29.0	41.0
36	36.08114241388411	39.0	35.0	40.0	29.0	41.0
37	35.84605293301312	38.0	35.0	40.0	27.0	41.0
38	35.75370901767289	38.0	35.0	40.0	27.0	41.0
39	35.599537502863626	38.0	35.0	40.0	26.0	41.0
40	35.47394165424312	38.0	34.0	40.0	26.0	41.0
41	35.35163917703084	38.0	34.0	40.0	26.0	41.0
42	35.16021814378634	38.0	34.0	40.0	25.0	41.0
43	31.780180166379203	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	11.0
10	33.0
11	42.0
12	29.0
13	30.0
14	43.0
15	104.0
16	183.0
17	341.0
18	813.0
19	1726.0
20	3614.0
21	6904.0
22	12488.0
23	20007.0
24	31149.0
25	44635.0
26	61529.0
27	82589.0
28	107988.0
29	138595.0
30	176140.0
31	220518.0
32	275475.0
33	347753.0
34	446742.0
35	591564.0
36	830730.0
37	1303906.0
38	2331573.0
39	1579445.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.21176501171794	18.748785788344545	12.646845626087568	26.39260357384995
2	18.72585358063909	21.21277955301228	34.31374323958285	25.747623626765787
3	18.553502256431752	22.004671857051573	30.782925996512354	28.65889989000432
4	14.770430728601266	15.74746347268363	33.522570468376415	35.95953533033868
5	14.53843941684417	36.96041710621999	34.046576056593345	14.454567420342492
6	36.23862122654352	35.43239629268832	14.073992578599098	14.254989902169065
7	30.31865323879136	30.952515243070955	19.947434644948846	18.78139687318884
8	28.17902951732577	34.928061803692415	18.541084512380724	18.351824166601098
9	26.99737091987166	13.56590955565134	18.047345724617145	41.389373799859854
10	15.597394455558518	27.722335065086384	33.602647509453156	23.07762296990194
11	37.56164481491875	20.89731082727475	21.090458971425495	20.450585386381007
12	21.83066096518134	25.020152722004312	29.819343874257225	23.32984243855712
13	31.953501467266708	19.536070761179857	23.448286827141057	25.062140944412377
14	22.55914153698248	19.950127090387948	25.54469215716175	31.94603921546782
15	25.69814994182229	27.984465518245845	21.37107677624223	24.946307763689635
16	25.17473622738723	26.032198862163273	23.562692547566343	25.230372362883152
17	24.687879423008944	26.192817159047628	24.878671677400472	24.24063174054296
18	24.6858252728248	25.34307209417246	25.2992154074726	24.67188722553014
19	25.576583709173185	25.346495677812698	25.402398736778874	23.674521876235247
20	25.27136252361983	25.171602777953794	25.11863587716043	24.438398821265956
21	24.95986283383132	25.26970295595693	25.23280949022027	24.537624719991477
22	25.48274270132587	24.98772732305237	24.771484496040365	24.7580454795814
23	24.994481647386667	25.00882588257085	24.89155363618238	25.105138833860103
24	24.91852451204649	25.003127646749302	24.960187784142935	25.118160057061274
25	25.089552824270818	24.66594527697488	25.128941444185955	25.115560454568346
26	24.75093138883067	25.018713656338587	25.26218267731668	24.968172277514064
27	25.019131449596376	25.298553901481103	24.994307566862588	24.688007082059933
28	24.901766360261735	25.279114909625516	25.057904984993094	24.761213745119655
29	24.83873760517655	25.581829335632122	25.079084782089485	24.500348277101843
30	24.91392878621078	25.481500926920763	24.898690937669656	24.7058793491988
31	24.89901588798127	24.82922120319352	25.136879516083997	25.134883392741216
32	24.439861097668224	25.29074348863405	25.17899539754305	25.090400016154675
33	24.63918329773967	24.866845807131313	25.20725446928535	25.286716425843668
34	24.8556234160123	24.69189488043105	25.6122934273461	24.84018827621055
35	24.656150346153318	24.779167249836423	25.59933023098629	24.965352173023973
36	24.45859216205922	24.470928668532345	25.893282603947544	25.17719656546089
37	24.212546749324744	24.07677554591072	26.232704809798456	25.47797289496608
38	23.794672253955166	24.08645442304956	26.64220022927565	25.476673093719615
39	23.653388500611953	23.857863484196155	27.213161137521062	25.27558687767083
40	23.77558142314774	23.688448318161637	27.521550588612676	25.01441967007795
41	23.37719234110684	23.563574555555014	27.90050067879799	25.158732424540155
42	22.92692726289014	23.805070663926873	27.90352967991698	25.36447239326601
43	23.019039070864935	22.992044984264282	27.8638277150585	26.125088229812288
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	837.0
1	931.5
2	1026.0
3	2279.0
4	3532.0
5	3532.0
6	4034.0
7	4536.0
8	4903.5
9	5271.0
10	7920.5
11	10570.0
12	10570.0
13	19521.5
14	28473.0
15	46190.0
16	63907.0
17	50242.5
18	36578.0
19	36578.0
20	43488.0
21	50398.0
22	47795.0
23	45192.0
24	55474.5
25	65757.0
26	65757.0
27	80743.0
28	95729.0
29	111564.5
30	127400.0
31	150670.0
32	173940.0
33	173940.0
34	200813.0
35	227686.0
36	262841.0
37	297996.0
38	338941.5
39	379887.0
40	379887.0
41	426569.5
42	473252.0
43	519404.5
44	565557.0
45	610536.5
46	655516.0
47	655516.0
48	697499.5
49	739483.0
50	758495.5
51	777508.0
52	774218.0
53	770928.0
54	770928.0
55	742643.0
56	714358.0
57	669107.5
58	623857.0
59	570475.0
60	517093.0
61	517093.0
62	454079.0
63	391065.0
64	333147.5
65	275230.0
66	227976.0
67	180722.0
68	180722.0
69	147654.5
70	114587.0
71	93839.5
72	73092.0
73	60146.0
74	47200.0
75	47200.0
76	38855.0
77	30510.0
78	25118.0
79	19726.0
80	15989.5
81	12253.0
82	12253.0
83	9682.5
84	7112.0
85	5556.5
86	4001.0
87	3374.5
88	2748.0
89	2748.0
90	2009.5
91	1271.0
92	917.5
93	564.0
94	413.0
95	262.0
96	262.0
97	162.5
98	63.0
99	46.0
100	29.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	8616702.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.71530554467432
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.34064257150531	43.15522163383878
2	9.882651766256902	10.616993184322096
3	3.557855047915032	5.733338129472535
4	1.688553692315859	3.628047100453374
5	1.0215732788057683	2.7437060403663303
6	0.6469230048474575	2.0849800121556
7	0.4599544565677914	1.7294615919821485
8	0.32889458909279906	1.4133338676087848
9	0.25537672851817045	1.234587510121758
>10	1.6277529148633636	16.541770720609662
>50	0.13572087278199024	5.015034410684089
>100	0.05278126533461182	4.6301813873522795
>500	0.0010386628019300962	0.36179292348233166
>1k	1.9464120499957483E-4	0.17770373934082878
>5k	0.0	0.0
>10k+	8.650718807681358E-5	0.9338477482094073
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	40178	0.46628048643204795	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15443	0.17922170222435452	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12803	0.1485835299862987	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11963	0.1388350206378264	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1605368271990838E-5	0.0
2	0.0	0.0	0.0	2.3210736543981675E-5	0.0
3	0.0	0.0	0.0	2.3210736543981675E-5	0.0
4	0.0	0.0	0.0	2.3210736543981675E-5	0.0
5	0.0	0.0	0.0	2.3210736543981675E-5	0.0
6	0.0	0.0	0.0	2.3210736543981675E-5	2.3210736543981675E-5
7	0.0	0.0	0.0	2.3210736543981675E-5	4.642147308796335E-5
8	0.0	0.0	0.0	2.3210736543981675E-5	5.802684135995419E-5
9	1.1605368271990838E-5	0.0	0.0	2.3210736543981675E-5	6.963220963194504E-5
10	2.3210736543981675E-5	0.0	0.0	2.3210736543981675E-5	8.123757790393587E-5
11	3.481610481597252E-5	0.0	0.0	2.3210736543981675E-5	8.123757790393587E-5
12	3.481610481597252E-5	0.0	0.0	2.3210736543981675E-5	9.28429461759267E-5
13	3.481610481597252E-5	1.1605368271990838E-5	0.0	2.3210736543981675E-5	9.28429461759267E-5
14	3.481610481597252E-5	1.1605368271990838E-5	0.0	2.3210736543981675E-5	1.2765905099189923E-4
15	3.481610481597252E-5	1.1605368271990838E-5	0.0	5.802684135995419E-5	1.3926441926389007E-4
16	4.642147308796335E-5	1.1605368271990838E-5	0.0	1.6247515580787174E-4	1.9729126062384425E-4
17	4.642147308796335E-5	1.1605368271990838E-5	0.0	3.017395750717618E-4	1.9729126062384425E-4
18	4.642147308796335E-5	1.1605368271990838E-5	0.0	3.4816104815972517E-4	3.1334494334375263E-4
19	4.642147308796335E-5	1.1605368271990838E-5	0.0	4.5260936260764267E-4	3.365556798877343E-4
20	4.642147308796335E-5	1.1605368271990838E-5	0.0	6.150845184155144E-4	3.365556798877343E-4
21	4.642147308796335E-5	1.1605368271990838E-5	0.0	9.516401983032487E-4	3.59766416431716E-4
22	5.802684135995419E-5	1.1605368271990838E-5	0.0	0.0016247515580787174	3.713717847037068E-4
23	5.802684135995419E-5	1.1605368271990838E-5	0.0	0.002019334079326406	4.874254674236152E-4
24	5.802684135995419E-5	1.1605368271990838E-5	1.1605368271990838E-5	0.002669234702557893	4.874254674236152E-4
25	5.802684135995419E-5	1.1605368271990838E-5	1.1605368271990838E-5	0.0030522118555335906	4.990308356956061E-4
26	5.802684135995419E-5	1.1605368271990838E-5	1.1605368271990838E-5	0.0037717446883970226	4.990308356956061E-4
27	5.802684135995419E-5	1.1605368271990838E-5	1.1605368271990838E-5	0.00859957788954521	4.990308356956061E-4
28	6.963220963194504E-5	1.1605368271990838E-5	1.1605368271990838E-5	0.036939887209746836	4.990308356956061E-4
29	6.963220963194504E-5	1.1605368271990838E-5	1.1605368271990838E-5	0.09691643043939549	4.990308356956061E-4
30	6.963220963194504E-5	1.1605368271990838E-5	1.1605368271990838E-5	0.17708631446230821	4.990308356956061E-4
31	6.963220963194504E-5	1.1605368271990838E-5	1.1605368271990838E-5	0.515661328429369	4.990308356956061E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	5325	0.0	19.003756	37
TCTTATA	9285	0.0	15.481421	37
AACGCAG	18240	0.0	14.970394	5
GGTATCA	11535	0.0	14.161681	1
TAGTACT	800	0.0	13.4125	4
TATACCG	415	0.0	13.373494	5
ACGCAGA	20765	0.0	13.150012	6
CTCTTAT	14785	0.0	13.075752	37
CGCAGAG	21050	0.0	12.998337	7
AGAGTAC	21375	0.0	12.774736	10
CAGAGTA	22190	0.0	12.45561	9
TAATACT	1190	0.0	12.436975	4
CAACGCA	22050	0.0	12.375284	4
TCAACGC	22150	0.0	12.336118	3
ATCAACG	22255	0.0	12.311166	2
GCAGAGT	22810	0.0	12.254931	8
TATCAAC	22255	0.0	12.219726	1
GTCTTAG	1305	0.0	12.191571	1
GAGTACT	12455	0.0	12.120434	11
TATACTG	1105	0.0	11.719458	5
>>END_MODULE