FastQCFastQC Report
Thu 9 Feb 2017
SRR2727374.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727374.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13199909
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT550070.4167225698298375No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT230020.17425877708702386No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176600.1337888011197653No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT174620.13228879077878491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC76800.021.51106637
TCTTATA139300.015.9899537
AACGCAG242400.015.584575
GGTATCA140900.014.9680641
ACGCAGA276050.013.7585586
CGCAGAG281600.013.5662297
AGAGTAC282200.013.46527310
CTCTTAT220600.013.4431137
TCAACGC284700.013.321043
CAACGCA285950.013.3016264
ATCAACG285350.013.2842112
TATCAAC287850.013.1238481
CAGAGTA296050.012.9790569
GCAGAGT308400.012.5912788
GTATTAG12050.012.5892111
TCTATAC12250.012.3836743
GAGTACT175300.012.37906511
TATACTG15850.012.3722395
TTATACT20200.011.9059414
TAGACAG26950.011.8756965
Produced by FastQC (version 0.11.5)