##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727374.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13199909
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14572812585299	33.0	31.0	34.0	30.0	34.0
2	32.2918203451251	34.0	31.0	34.0	30.0	34.0
3	32.377514117710966	34.0	31.0	34.0	30.0	34.0
4	35.9484911600527	37.0	35.0	37.0	35.0	37.0
5	35.83107307785228	37.0	35.0	37.0	35.0	37.0
6	35.84496461301362	37.0	35.0	37.0	35.0	37.0
7	35.79281092013589	37.0	35.0	37.0	35.0	37.0
8	35.76736907807471	37.0	35.0	37.0	35.0	37.0
9	37.49502560964625	39.0	37.0	39.0	35.0	39.0
10	37.33895703371894	39.0	37.0	39.0	34.0	39.0
11	37.44194941040882	39.0	37.0	39.0	35.0	39.0
12	37.34936392364523	39.0	37.0	39.0	34.0	39.0
13	37.40773629575779	39.0	37.0	39.0	34.0	39.0
14	38.59442235548745	40.0	38.0	41.0	34.0	41.0
15	38.55778142106889	40.0	38.0	41.0	34.0	41.0
16	38.4997518543499	40.0	38.0	41.0	34.0	41.0
17	38.457031408322585	40.0	38.0	41.0	34.0	41.0
18	38.411068818732005	40.0	38.0	41.0	34.0	41.0
19	38.415943700823995	40.0	38.0	41.0	34.0	41.0
20	38.304788919378154	40.0	38.0	41.0	34.0	41.0
21	38.230373103329725	40.0	38.0	41.0	34.0	41.0
22	38.16056716754638	40.0	38.0	41.0	34.0	41.0
23	38.05656690512033	40.0	38.0	41.0	33.0	41.0
24	37.95576204351106	40.0	37.0	41.0	33.0	41.0
25	37.874239208770305	40.0	37.0	41.0	33.0	41.0
26	37.53322913059476	40.0	37.0	41.0	32.0	41.0
27	37.330733946726454	39.0	37.0	41.0	31.0	41.0
28	37.19636953557786	39.0	37.0	41.0	31.0	41.0
29	37.170550948495176	39.0	37.0	40.0	31.0	41.0
30	37.10157850330635	39.0	36.0	40.0	31.0	41.0
31	36.907255496988654	39.0	36.0	40.0	30.0	41.0
32	36.81069036157749	39.0	36.0	40.0	30.0	41.0
33	36.648940155572284	39.0	36.0	40.0	30.0	41.0
34	36.52827439946745	39.0	36.0	40.0	30.0	41.0
35	36.38888745369381	39.0	35.0	40.0	30.0	41.0
36	36.253305382635595	39.0	35.0	40.0	29.0	41.0
37	36.027884737690236	39.0	35.0	40.0	28.0	41.0
38	35.93559970754344	38.0	35.0	40.0	28.0	41.0
39	35.78649330082503	38.0	35.0	40.0	27.0	41.0
40	35.66443291389357	38.0	35.0	40.0	27.0	41.0
41	35.546722481192866	38.0	35.0	40.0	26.0	41.0
42	35.36405713099992	38.0	34.0	40.0	26.0	41.0
43	31.934503942413542	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	12.0
10	52.0
11	68.0
12	57.0
13	51.0
14	71.0
15	133.0
16	216.0
17	505.0
18	1019.0
19	2163.0
20	4907.0
21	9346.0
22	17168.0
23	28372.0
24	43905.0
25	62864.0
26	88385.0
27	117727.0
28	155493.0
29	200063.0
30	254807.0
31	321550.0
32	404984.0
33	510202.0
34	659912.0
35	876900.0
36	1233538.0
37	1957367.0
38	3608946.0
39	2639126.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.506876373162875	18.521673141837567	12.446835807731704	26.52461467726785
2	18.651613431577445	20.93330340383407	34.43316162255361	25.981921542034875
3	18.486195624530442	21.819392845814317	30.70165104926102	28.99276048039422
4	14.806617227436947	15.557410282146641	33.432594118641276	36.203378371775145
5	14.54636543327685	36.8682768949392	34.106000276214026	14.479357395569926
6	36.56062325884216	35.17474249254294	14.058983285415074	14.205650963199822
7	30.535755966196433	30.867303706411914	19.919478232766604	18.677462094625046
8	28.273808554286244	35.00520344496314	18.504180597002602	18.21680740374801
9	27.14849019034904	13.526479614367037	17.93629031836507	41.38873987691885
10	15.634175962879743	27.615372196884085	33.657830519892215	23.09262132034395
11	37.672479408759564	20.810287404254073	21.070804351757275	20.446428835229092
12	21.86318102647526	24.75775401178902	29.888804536455517	23.490260425280205
13	31.904553281390047	19.408656529374557	23.49523773232073	25.191552456914668
14	22.631906022988492	19.74071942465664	25.37749313271781	32.24988141963706
15	25.701419608271543	27.835373713561207	21.34150318763561	25.121703490531637
16	25.3536975141268	25.967709322844573	23.457623836649176	25.220969326379443
17	24.728382597183057	26.10902090309865	24.804254332359413	24.35834216735888
18	24.77196623097932	25.419152510824127	25.169347758382273	24.639533499814277
19	25.640244944112872	25.274136359576417	25.3231973038602	23.76242139245051
20	25.373561287430086	25.065097039684137	25.020717945858568	24.540623727027207
21	25.019528543719506	25.197211586837454	25.180128135731845	24.603131733711194
22	25.615070528137736	24.813928641477755	24.71484462506522	24.856156205319294
23	25.06232429329626	24.8962322391768	24.861875941720506	25.17956752580643
24	25.034596829417538	24.79089060386704	24.93042186881743	25.24409069789799
25	25.267204493606737	24.460032262343624	25.01252091965179	25.26024232439784
26	24.904148960420862	24.823512040878466	25.221522360495065	25.050816638205614
27	25.126923223485857	25.1743250654228	24.846125833140214	24.85262587795113
28	25.04468780807504	25.203143445913152	24.849951617090692	24.902217128921116
29	24.997626877579233	25.465175555376934	24.973134284486356	24.564063282557477
30	25.22271176263412	25.247795268891625	24.706662750478053	24.8228302179962
31	25.039369589593385	24.733981120627423	25.044005985192776	25.182643304586417
32	24.613828777152932	25.12442320625089	25.0634606647667	25.198287351829475
33	24.738799335662087	24.706253656748693	25.093968450843107	25.46097855674611
34	24.983543447155583	24.533600951339892	25.54259275575309	24.940262845751437
35	24.792913345084425	24.556631413140803	25.52412293145354	25.12633231032123
36	24.66524579828543	24.266349108921887	25.81029914675927	25.258105946033417
37	24.37134225698071	23.889748027808373	26.130899841809512	25.6080098734014
38	24.01891558494835	23.893210172888313	26.528046519108578	25.559827723054756
39	23.81652025025324	23.691686056320542	27.090694337362475	25.401099356063746
40	24.00374123791308	23.56478366631164	27.32752172761191	25.103953368163374
41	23.53873045639936	23.476820938689805	27.704675842841038	25.279772762069797
42	23.13086400822915	23.710640732447473	27.70607736765458	25.452417891668798
43	23.222319184170136	22.900945756519988	27.652145177667514	26.22458988164237
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1388.0
1	1508.0
2	1628.0
3	3557.0
4	5486.0
5	5486.0
6	6299.5
7	7113.0
8	7470.0
9	7827.0
10	11892.5
11	15958.0
12	15958.0
13	28272.0
14	40586.0
15	64527.5
16	88469.0
17	70145.0
18	51821.0
19	51821.0
20	61545.0
21	71269.0
22	66896.0
23	62523.0
24	76267.0
25	90011.0
26	90011.0
27	110789.0
28	131567.0
29	154613.5
30	177660.0
31	210819.5
32	243979.0
33	243979.0
34	285039.5
35	326100.0
36	381766.5
37	437433.0
38	502420.5
39	567408.0
40	567408.0
41	641537.0
42	715666.0
43	792500.0
44	869334.0
45	946240.0
46	1023146.0
47	1023146.0
48	1091318.5
49	1159491.0
50	1193079.5
51	1226668.0
52	1219374.0
53	1212080.0
54	1212080.0
55	1168458.0
56	1124836.0
57	1050052.0
58	975268.0
59	891816.5
60	808365.0
61	808365.0
62	707166.5
63	605968.0
64	514666.0
65	423364.0
66	349258.5
67	275153.0
68	275153.0
69	224284.0
70	173415.0
71	140296.5
72	107178.0
73	87296.0
74	67414.0
75	67414.0
76	54618.5
77	41823.0
78	34163.5
79	26504.0
80	21240.5
81	15977.0
82	15977.0
83	12606.0
84	9235.0
85	7196.5
86	5158.0
87	4180.0
88	3202.0
89	3202.0
90	2317.0
91	1432.0
92	1016.5
93	601.0
94	436.0
95	271.0
96	271.0
97	180.5
98	90.0
99	67.0
100	44.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1.3199909E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.4409749400953
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.68590641996313	37.631810460880224
2	10.765594754100839	10.429918113972402
3	4.020057032666666	5.842064459314796
4	2.053710541036663	3.979349634102662
5	1.1880365256425047	2.8774823783283225
6	0.7943210366302036	2.308661125787653
7	0.5489195952382235	1.8613140249943423
8	0.4151602900919391	1.6088615366773054
9	0.3295923004358638	1.4369195129275834
>10	1.9349208190990397	17.695801491589986
>50	0.17752405900595056	5.914632703924584
>100	0.08430418389534337	6.80622114880331
>500	0.0016078458987160227	0.5083715097562006
>1k	2.81944501191592E-4	0.24023476979007077
>5k	0.0	0.0
>10k+	6.265179360536723E-5	0.8583571291502735
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	55007	0.4167225698298375	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23002	0.17425877708702386	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17660	0.1337888011197653	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	17462	0.13228879077878491	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.818228822638096E-5
2	0.0	0.0	0.0	0.0	6.818228822638096E-5
3	0.0	0.0	0.0	1.5151619605862434E-5	6.818228822638096E-5
4	0.0	0.0	0.0	1.5151619605862434E-5	6.818228822638096E-5
5	7.575809802931217E-6	0.0	0.0	1.5151619605862434E-5	7.575809802931217E-5
6	7.575809802931217E-6	0.0	0.0	1.5151619605862434E-5	9.848552743810582E-5
7	7.575809802931217E-6	0.0	0.0	1.5151619605862434E-5	9.848552743810582E-5
8	2.2727429408793653E-5	0.0	0.0	1.5151619605862434E-5	1.1363714704396825E-4
9	3.030323921172487E-5	0.0	0.0	2.2727429408793653E-5	1.6666781566448678E-4
10	3.7879049014656084E-5	0.0	0.0	2.2727429408793653E-5	1.74243625467418E-4
11	4.5454858817587306E-5	0.0	0.0	2.2727429408793653E-5	1.8181943527034923E-4
12	4.5454858817587306E-5	0.0	0.0	3.7879049014656084E-5	2.0454686467914285E-4
13	5.303066862051852E-5	0.0	0.0	3.7879049014656084E-5	2.0454686467914285E-4
14	5.303066862051852E-5	0.0	0.0	3.7879049014656084E-5	2.9545658231431746E-4
15	5.303066862051852E-5	1.5151619605862434E-5	0.0	4.5454858817587306E-5	3.106082019201799E-4
16	7.575809802931217E-5	1.5151619605862434E-5	0.0	8.333390783224339E-5	3.7879049014656087E-4
17	7.575809802931217E-5	1.5151619605862434E-5	0.0	9.848552743810582E-5	3.7879049014656087E-4
18	7.575809802931217E-5	1.5151619605862434E-5	0.0	1.0606133724103704E-4	4.015179195553545E-4
19	7.575809802931217E-5	1.5151619605862434E-5	0.0	1.8939524507328043E-4	4.090937293582857E-4
20	7.575809802931217E-5	1.5151619605862434E-5	0.0	3.6363887054069845E-4	4.1666953916121697E-4
21	8.333390783224339E-5	1.5151619605862434E-5	0.0	5.757615450227725E-4	4.469727783729418E-4
22	8.333390783224339E-5	1.5151619605862434E-5	0.0	0.0010303101331986455	4.54548588175873E-4
23	8.333390783224339E-5	1.5151619605862434E-5	0.0	0.0012348569978777883	5.606099254169101E-4
24	9.090971763517461E-5	1.5151619605862434E-5	0.0	0.0016212232978272804	5.606099254169101E-4
25	9.848552743810582E-5	1.5151619605862434E-5	0.0	0.0018030427330976296	5.606099254169101E-4
26	1.0606133724103704E-4	1.5151619605862434E-5	0.0	0.0022954703702881586	5.606099254169101E-4
27	1.0606133724103704E-4	1.5151619605862434E-5	0.0	0.006447014142294466	5.681857352198413E-4
28	1.0606133724103704E-4	1.5151619605862434E-5	0.0	0.03178809793309939	5.833373548257037E-4
29	1.0606133724103704E-4	1.5151619605862434E-5	0.0	0.0828036011460382	5.833373548257037E-4
30	1.1363714704396825E-4	1.5151619605862434E-5	0.0	0.1516298332056683	5.833373548257037E-4
31	1.1363714704396825E-4	1.5151619605862434E-5	0.0	0.48568516646592036	5.833373548257037E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	7680	0.0	21.511066	37
TCTTATA	13930	0.0	15.98995	37
AACGCAG	24240	0.0	15.58457	5
GGTATCA	14090	0.0	14.968064	1
ACGCAGA	27605	0.0	13.758558	6
CGCAGAG	28160	0.0	13.566229	7
AGAGTAC	28220	0.0	13.465273	10
CTCTTAT	22060	0.0	13.44311	37
TCAACGC	28470	0.0	13.32104	3
CAACGCA	28595	0.0	13.301626	4
ATCAACG	28535	0.0	13.284211	2
TATCAAC	28785	0.0	13.123848	1
CAGAGTA	29605	0.0	12.979056	9
GCAGAGT	30840	0.0	12.591278	8
GTATTAG	1205	0.0	12.589211	1
TCTATAC	1225	0.0	12.383674	3
GAGTACT	17530	0.0	12.379065	11
TATACTG	1585	0.0	12.372239	5
TTATACT	2020	0.0	11.905941	4
TAGACAG	2695	0.0	11.875696	5
>>END_MODULE