FastQCFastQC Report
Thu 9 Feb 2017
SRR2727373.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727373.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6323014
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT182030.28788485997342406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT74860.11839290566176194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC36500.019.81780837
TATACCG2750.016.8181825
GGTATCA51600.016.4563961
TCTTATA65850.016.2946137
AACGCAG91650.016.0070925
ACGCAGA103150.014.3839076
CAACGCA102800.014.3608944
TCAACGC104600.014.1491393
ATCAACG105350.014.0308492
CGCAGAG105800.014.0236297
AGAGTAC108600.013.73020210
TATCAAC108650.013.5365861
CAGAGTA113050.013.4679359
TAGTACT5900.013.1694924
CTCTTAT103400.013.09671237
TAGACAG12350.012.882595
ATTAGAC4200.012.7738093
GCAGAGT119850.012.6883598
CTAGTAC4450.012.47190953
GTATCAA67750.012.3424352
Produced by FastQC (version 0.11.5)