##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727373.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6323014
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1072844374534	33.0	31.0	34.0	30.0	34.0
2	32.26069877435033	34.0	31.0	34.0	30.0	34.0
3	32.34661602836875	34.0	31.0	34.0	30.0	34.0
4	35.928501818911045	37.0	35.0	37.0	35.0	37.0
5	35.80940592571834	37.0	35.0	37.0	35.0	37.0
6	35.82173422358388	37.0	35.0	37.0	35.0	37.0
7	35.77300999175393	37.0	35.0	37.0	35.0	37.0
8	35.7430962512498	37.0	35.0	37.0	35.0	37.0
9	37.469137344943405	39.0	37.0	39.0	35.0	39.0
10	37.309487690522275	39.0	37.0	39.0	34.0	39.0
11	37.41123631862906	39.0	37.0	39.0	35.0	39.0
12	37.31602681885569	39.0	37.0	39.0	34.0	39.0
13	37.37605689312091	39.0	37.0	39.0	34.0	39.0
14	38.55950943648077	40.0	38.0	41.0	34.0	41.0
15	38.52262275554032	40.0	38.0	41.0	34.0	41.0
16	38.462718728758155	40.0	38.0	41.0	34.0	41.0
17	38.41568467189856	40.0	38.0	41.0	34.0	41.0
18	38.36525413355087	40.0	38.0	41.0	34.0	41.0
19	38.36775863535966	40.0	38.0	41.0	34.0	41.0
20	38.25420867326879	40.0	38.0	41.0	34.0	41.0
21	38.17909939785046	40.0	38.0	41.0	34.0	41.0
22	38.109309262955925	40.0	38.0	41.0	33.0	41.0
23	38.00381020823297	40.0	37.0	41.0	33.0	41.0
24	37.90282023731088	40.0	37.0	41.0	33.0	41.0
25	37.82331685490495	40.0	37.0	41.0	33.0	41.0
26	37.48006662645378	39.0	37.0	41.0	32.0	41.0
27	37.27613413476548	39.0	37.0	41.0	31.0	41.0
28	37.14202641335287	39.0	36.0	41.0	31.0	41.0
29	37.11674574815112	39.0	36.0	40.0	31.0	41.0
30	37.05382448939699	39.0	36.0	40.0	31.0	41.0
31	36.864482191562445	39.0	36.0	40.0	30.0	41.0
32	36.773212743163306	39.0	36.0	40.0	30.0	41.0
33	36.61078213649377	39.0	36.0	40.0	30.0	41.0
34	36.49855527759388	39.0	36.0	40.0	30.0	41.0
35	36.356250199667436	39.0	35.0	40.0	30.0	41.0
36	36.22211843908617	39.0	35.0	40.0	29.0	41.0
37	36.001901466610704	38.0	35.0	40.0	28.0	41.0
38	35.916916995597354	38.0	35.0	40.0	27.0	41.0
39	35.772305264546304	38.0	35.0	40.0	27.0	41.0
40	35.65658813977005	38.0	35.0	40.0	27.0	41.0
41	35.54946438518086	38.0	35.0	40.0	26.0	41.0
42	35.3693018867268	38.0	34.0	40.0	26.0	41.0
43	31.942863166205232	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	10.0
11	14.0
12	19.0
13	16.0
14	22.0
15	56.0
16	108.0
17	200.0
18	486.0
19	1091.0
20	2331.0
21	4606.0
22	8446.0
23	14026.0
24	21492.0
25	31516.0
26	43197.0
27	57914.0
28	76096.0
29	98125.0
30	124442.0
31	156732.0
32	196804.0
33	247777.0
34	319755.0
35	423914.0
36	592164.0
37	930711.0
38	1726043.0
39	1244894.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.255876706899585	18.691956082969295	12.31948877544791	26.732678434683205
2	18.78148617099377	20.815421253218798	34.309175972091786	26.093916603695643
3	18.486816571970266	21.851746651201466	30.638078612509794	29.023358164318473
4	14.844787628178588	15.619449838320776	33.4781640527761	36.057598480724536
5	14.550798084584345	36.76893645973266	34.19771014266298	14.482555313020026
6	36.580339692431494	35.20561554979951	14.112067441255071	14.101977316513928
7	30.51592167912328	30.831008756267188	19.970112354646062	18.682957209963476
8	28.168639196433855	35.00197848684188	18.509748673654684	18.319633643069587
9	27.151276274257814	13.457253139088415	17.940447387907096	41.451023198746675
10	15.572652535642021	27.608147000781592	33.787510196877626	23.031690266698764
11	37.74870655038879	20.76485992281529	21.02868979888389	20.457743727912035
12	21.828608951364018	24.656374317690897	29.890760956720957	23.624255774224128
13	31.904515789463694	19.49037278740803	23.332717593223737	25.272393829904537
14	22.6642863672293	19.712450423168445	25.16103870717351	32.46222450242875
15	25.741584630367733	27.840646881376507	21.19367440907137	25.22409407918439
16	25.463410329314467	26.00785005378764	23.335263847272834	25.193475769625056
17	24.774688147139955	26.081643975483843	24.608944405310506	24.534723472065696
18	24.83393204569846	25.506554310966255	24.993555288664552	24.66595835467073
19	25.673326043560873	25.36186065695885	25.054839353510843	23.909973945969437
20	25.468044195378976	25.05338435119707	24.78930143124782	24.68927002217613
21	25.08648565383534	25.200387030615463	24.913308748011627	24.79981856753757
22	25.740034736598716	24.82181756991207	24.44036973506622	24.997777958422994
23	25.245840037678235	24.882326687873853	24.549779582964707	25.32205369148321
24	25.125059030392784	24.79534285389847	24.699486668857602	25.38011144685114
25	25.382483733232284	24.447075397903596	24.75020615168652	25.420234717177596
26	25.01243868825848	24.81648783317576	24.92115310831195	25.249920370253808
27	25.24319889217389	25.112153792479347	24.650491047465657	24.99415626788111
28	25.176442753408423	25.13793263782114	24.597620691651166	25.088003917119273
29	25.108721884847956	25.422907493167024	24.73301498304448	24.735355638940543
30	25.352529663859674	25.19730305831997	24.508928811481358	24.941238466338998
31	25.221911575713733	24.70538575432539	24.82618257685338	25.246520093107495
32	24.764771990066762	25.03021502087454	24.855899417587878	25.34911357147082
33	24.878119833357953	24.641950816493527	24.909386567861468	25.570542782287053
34	25.107029653896067	24.453986658894003	25.31137840276805	25.127605284441884
35	24.903202808027945	24.4681887466958	25.330040388966403	25.298568056309854
36	24.7317497636412	24.224618196322197	25.642312352938013	25.40131968709859
37	24.479370123172274	23.779387488308583	26.022969425656818	25.718272962862333
38	24.120079443126333	23.812567867159554	26.377215043332182	25.690137646381935
39	23.93222599222459	23.632147580252074	26.956812051973948	25.47881437554938
40	24.094079184388963	23.4323536212319	27.232455914220655	25.241111280158478
41	23.636512587193387	23.376636521760034	27.553647674985378	25.4332032160612
42	23.22636008713566	23.542744014167926	27.601915795220442	25.628980103475968
43	23.29216731134867	22.78739221516827	27.529750843505962	26.3906896299771
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	383.0
1	435.5
2	488.0
3	1071.5
4	1655.0
5	1655.0
6	1947.0
7	2239.0
8	2413.5
9	2588.0
10	3920.5
11	5253.0
12	5253.0
13	9305.5
14	13358.0
15	21718.0
16	30078.0
17	24539.0
18	19000.0
19	19000.0
20	23256.5
21	27513.0
22	27738.5
23	27964.0
24	34992.5
25	42021.0
26	42021.0
27	52474.0
28	62927.0
29	74550.5
30	86174.0
31	103948.5
32	121723.0
33	121723.0
34	142743.5
35	163764.0
36	190670.0
37	217576.0
38	248228.5
39	278881.0
40	278881.0
41	313312.0
42	347743.0
43	383236.5
44	418730.0
45	454086.0
46	489442.0
47	489442.0
48	522026.0
49	554610.0
50	570446.0
51	586282.0
52	583040.0
53	579798.0
54	579798.0
55	559546.0
56	539294.0
57	502385.0
58	465476.0
59	425637.0
60	385798.0
61	385798.0
62	338296.0
63	290794.0
64	247341.5
65	203889.0
66	169037.5
67	134186.0
68	134186.0
69	109717.5
70	85249.0
71	69189.0
72	53129.0
73	43213.5
74	33298.0
75	33298.0
76	27193.5
77	21089.0
78	17145.0
79	13201.0
80	10514.5
81	7828.0
82	7828.0
83	6160.0
84	4492.0
85	3520.5
86	2549.0
87	2001.0
88	1453.0
89	1453.0
90	1032.0
91	611.0
92	463.5
93	316.0
94	216.0
95	116.0
96	116.0
97	78.5
98	41.0
99	28.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	6323014.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.8858640750194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.60408154843363	46.65836908404409
2	10.114804074540379	11.710083476085936
3	3.4758405330623714	6.036060979298743
4	1.6478552936023505	3.8155011016306744
5	0.9563908262058585	2.7680754684173916
6	0.6360930551989477	2.209247767938606
7	0.44469694901718565	1.801916700176925
8	0.33302677512280554	1.542203411048062
9	0.2382415798002824	1.2411737734803332
>10	1.4275900791168463	15.291865260904103
>50	0.0920168475204201	3.6019793084996405
>100	0.028842812277763096	2.569495638053197
>500	3.8288723967850723E-4	0.14574128683882528
>1k	2.7347772305415415E-5	0.02121286236770648
>5k	8.204331690818441E-5	0.2989113934236383
>10k+	2.7347772302728138E-5	0.28816248779210296
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	18203	0.28788485997342406	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7486	0.11839290566176194	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.744572762293425E-5
2	0.0	0.0	0.0	0.0	4.744572762293425E-5
3	0.0	0.0	0.0	0.0	4.744572762293425E-5
4	0.0	0.0	0.0	0.0	4.744572762293425E-5
5	0.0	1.5815242540978085E-5	0.0	0.0	4.744572762293425E-5
6	0.0	1.5815242540978085E-5	0.0	0.0	4.744572762293425E-5
7	0.0	1.5815242540978085E-5	0.0	0.0	4.744572762293425E-5
8	0.0	1.5815242540978085E-5	0.0	0.0	6.326097016391234E-5
9	0.0	1.5815242540978085E-5	0.0	0.0	1.107066977868466E-4
10	0.0	1.5815242540978085E-5	0.0	0.0	1.107066977868466E-4
11	0.0	1.5815242540978085E-5	0.0	0.0	1.107066977868466E-4
12	0.0	1.5815242540978085E-5	0.0	0.0	1.107066977868466E-4
13	0.0	1.5815242540978085E-5	0.0	1.5815242540978085E-5	1.107066977868466E-4
14	1.5815242540978085E-5	3.163048508195617E-5	0.0	1.5815242540978085E-5	2.5304388065564937E-4
15	3.163048508195617E-5	3.163048508195617E-5	0.0	1.5815242540978085E-5	2.5304388065564937E-4
16	3.163048508195617E-5	3.163048508195617E-5	0.0	4.744572762293425E-5	2.688591231966274E-4
17	3.163048508195617E-5	3.163048508195617E-5	0.0	9.48914552458685E-5	2.688591231966274E-4
18	4.744572762293425E-5	3.163048508195617E-5	0.0	1.107066977868466E-4	2.846743657376055E-4
19	4.744572762293425E-5	3.163048508195617E-5	0.0	2.055981530327151E-4	2.846743657376055E-4
20	4.744572762293425E-5	3.163048508195617E-5	0.0	2.846743657376055E-4	2.846743657376055E-4
21	4.744572762293425E-5	3.163048508195617E-5	0.0	5.851639740161891E-4	2.846743657376055E-4
22	4.744572762293425E-5	3.163048508195617E-5	0.0	0.001107066977868466	2.846743657376055E-4
23	6.326097016391234E-5	3.163048508195617E-5	0.0	0.0013917413436060715	4.111963060654302E-4
24	6.326097016391234E-5	3.163048508195617E-5	0.0	0.0018503833772944359	4.111963060654302E-4
25	6.326097016391234E-5	3.163048508195617E-5	0.0	0.002024351045245195	4.111963060654302E-4
26	6.326097016391234E-5	3.163048508195617E-5	0.0	0.0026411455043433402	4.270115486064083E-4
27	6.326097016391234E-5	3.163048508195617E-5	0.0	0.006816369535161554	4.270115486064083E-4
28	6.326097016391234E-5	3.163048508195617E-5	0.0	0.03235798623884116	4.428267911473864E-4
29	6.326097016391234E-5	3.163048508195617E-5	0.0	0.08290350139980712	4.428267911473864E-4
30	9.48914552458685E-5	3.163048508195617E-5	0.0	0.14882143231060377	4.428267911473864E-4
31	9.48914552458685E-5	3.163048508195617E-5	0.0	0.4614255163755766	4.428267911473864E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	3650	0.0	19.817808	37
TATACCG	275	0.0	16.818182	5
GGTATCA	5160	0.0	16.456396	1
TCTTATA	6585	0.0	16.29461	37
AACGCAG	9165	0.0	16.007092	5
ACGCAGA	10315	0.0	14.383907	6
CAACGCA	10280	0.0	14.360894	4
TCAACGC	10460	0.0	14.149139	3
ATCAACG	10535	0.0	14.030849	2
CGCAGAG	10580	0.0	14.023629	7
AGAGTAC	10860	0.0	13.730202	10
TATCAAC	10865	0.0	13.536586	1
CAGAGTA	11305	0.0	13.467935	9
TAGTACT	590	0.0	13.169492	4
CTCTTAT	10340	0.0	13.096712	37
TAGACAG	1235	0.0	12.88259	5
ATTAGAC	420	0.0	12.773809	3
GCAGAGT	11985	0.0	12.688359	8
CTAGTAC	445	0.0	12.4719095	3
GTATCAA	6775	0.0	12.342435	2
>>END_MODULE