FastQCFastQC Report
Thu 9 Feb 2017
SRR2727371.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727371.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12065859
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT434050.35973402308115815No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT169450.1404375768024473No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT136440.1130793920267094No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128010.10609273653869153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC83950.021.5080437
TCTTATA152650.016.13069237
AACGCAG205350.015.5315315
TATACCG4650.015.1182795
GGTATCA128500.015.1023331
TATACTG15450.013.8899675
ACGCAGA233300.013.6311626
CGCAGAG237350.013.4531287
AGAGTAC236650.013.36784410
CAGAGTA245200.013.1884179
CTCTTAT238700.013.11353237
TCAACGC248300.012.8821993
CAACGCA247900.012.8731354
ATCAACG248150.012.8601662
GCAGAGT256850.012.6766618
TATCAAC250850.012.6553731
GTATTAG9500.012.4631581
GAGTACT133450.012.35181611
TAGATCG4000.012.0250015
CTATACT13700.011.8832124
Produced by FastQC (version 0.11.5)