##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727371.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12065859
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.152925788375285	33.0	31.0	34.0	30.0	34.0
2	32.299106429140274	34.0	31.0	34.0	30.0	34.0
3	32.383877600426125	34.0	31.0	34.0	30.0	34.0
4	35.95915326045166	37.0	35.0	37.0	35.0	37.0
5	35.84115701998507	37.0	35.0	37.0	35.0	37.0
6	35.85289012576726	37.0	35.0	37.0	35.0	37.0
7	35.80229894945731	37.0	35.0	37.0	35.0	37.0
8	35.77026981667861	37.0	35.0	37.0	35.0	37.0
9	37.50352005605237	39.0	37.0	39.0	35.0	39.0
10	37.342054386678974	39.0	37.0	39.0	34.0	39.0
11	37.446329018099746	39.0	37.0	39.0	35.0	39.0
12	37.35258227366987	39.0	37.0	39.0	34.0	39.0
13	37.41303234191614	39.0	37.0	39.0	35.0	39.0
14	38.598485031194215	40.0	38.0	41.0	34.0	41.0
15	38.56121076833402	40.0	38.0	41.0	34.0	41.0
16	38.49992951185655	40.0	38.0	41.0	34.0	41.0
17	38.4563907965442	40.0	38.0	41.0	34.0	41.0
18	38.4059376957745	40.0	38.0	41.0	34.0	41.0
19	38.41038155675447	40.0	38.0	41.0	34.0	41.0
20	38.29823860862289	40.0	38.0	41.0	34.0	41.0
21	38.22249969935833	40.0	38.0	41.0	34.0	41.0
22	38.15397751622989	40.0	38.0	41.0	33.0	41.0
23	38.04680843692935	40.0	38.0	41.0	33.0	41.0
24	37.94397323887176	40.0	37.0	41.0	33.0	41.0
25	37.86436340752863	40.0	37.0	41.0	33.0	41.0
26	37.51811114318508	40.0	37.0	41.0	32.0	41.0
27	37.31901591092686	39.0	37.0	41.0	31.0	41.0
28	37.17703969522601	39.0	37.0	41.0	31.0	41.0
29	37.148118173766164	39.0	37.0	40.0	31.0	41.0
30	37.07976522848477	39.0	36.0	40.0	31.0	41.0
31	36.884049697580586	39.0	36.0	40.0	30.0	41.0
32	36.79125050276155	39.0	36.0	40.0	30.0	41.0
33	36.62787448452696	39.0	36.0	40.0	30.0	41.0
34	36.503928646936785	39.0	36.0	40.0	30.0	41.0
35	36.363292576185415	39.0	35.0	40.0	30.0	41.0
36	36.22382111377234	39.0	35.0	40.0	29.0	41.0
37	36.00413265230432	39.0	35.0	40.0	28.0	41.0
38	35.91393136617957	38.0	35.0	40.0	27.0	41.0
39	35.76547421944844	38.0	35.0	40.0	27.0	41.0
40	35.64600406817285	38.0	35.0	40.0	27.0	41.0
41	35.52472550856098	38.0	35.0	40.0	26.0	41.0
42	35.34403708844932	38.0	34.0	40.0	26.0	41.0
43	31.91888443251326	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	16.0
11	22.0
12	25.0
13	35.0
14	49.0
15	101.0
16	224.0
17	491.0
18	981.0
19	2237.0
20	4612.0
21	9007.0
22	16199.0
23	26733.0
24	40960.0
25	58796.0
26	81282.0
27	108781.0
28	142249.0
29	182853.0
30	233899.0
31	294326.0
32	371454.0
33	467107.0
34	604708.0
35	802403.0
36	1130329.0
37	1779363.0
38	3281647.0
39	2424964.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.4442304522206	18.563875145565685	12.448405041033547	26.543489361180168
2	18.62014963045731	20.874899996759453	34.68567799441383	25.819272378369412
3	18.340617108156163	21.82190260966915	30.794442401490024	29.043037880684665
4	14.72748852775422	15.511850420264317	33.62867078091995	36.13199027106152
5	14.484588291641732	36.93321793334399	34.202256134436844	14.379937640577435
6	36.5586818145314	35.3373265840418	14.045473264688408	14.058518336738397
7	30.69290798110603	30.961898361318497	19.85711087789108	18.488082779684397
8	28.22920440227256	35.298854395696154	18.45070458721588	18.021236614815408
9	27.338683470443335	13.527532519649036	17.801360019207916	41.33242399069971
10	15.565041825865858	27.64423983406403	33.627336437463754	23.163381902606353
11	37.85885447525949	20.78418121743342	21.017691322267236	20.339272985039855
12	21.90149909757772	24.714377981708555	29.84740663719011	23.536716283523617
13	31.960418234623827	19.386584908708116	23.40735955890086	25.2456372977672
14	22.653206870725075	19.64384798463168	25.324877408230943	32.378067736412305
15	25.793505460324045	27.82698687262962	21.228169498748496	25.151338168297837
16	25.37496915884729	26.031126337544634	23.408006011010073	25.185898492597996
17	24.903556389976046	26.07307942186296	24.603602611301856	24.419761576859138
18	24.89989316135718	25.470486601907083	24.99365358073553	24.635966656000207
19	25.782225699803057	25.338486053914604	25.093488992370954	23.78579925391139
20	25.427240613370337	25.103111183381145	24.809605350103958	24.660042853144564
21	25.085416628853363	25.279053899104902	25.017083325770674	24.61844614627106
22	25.78081676571888	24.85603387210144	24.429566100515512	24.93358326166417
23	25.170524535385336	24.980865431959714	24.643790384091176	25.20481964856377
24	25.08947767415482	24.824059356238127	24.753380592297656	25.333082377309395
25	25.303262701810127	24.487299246576644	24.797505092675127	25.41193295893811
26	24.89314685344823	24.862747028620173	25.083651317324364	25.160454800607233
27	25.256278894026522	25.21609940908476	24.618595327527032	24.909026369361683
28	25.099738029426668	25.303146671944365	24.638055193583813	24.959060105045154
29	25.099365076286734	25.536888836509693	24.752941336377294	24.61080475082628
30	25.323882866524468	25.32090752925258	24.440124818299303	24.915084785923653
31	25.091930877030805	24.836640308825093	24.816542278506653	25.254886535637453
32	24.675209614168374	25.155523531312607	24.882016274183215	25.287250580335808
33	24.82685236086382	24.73040667887798	24.956698068492265	25.48604289176593
34	25.041383294798987	24.537739086790257	25.470635783163054	24.950241835247702
35	24.84114061004691	24.502300250649373	25.41978155057174	25.236777588731975
36	24.62956014984097	24.190420259344982	25.774451698797407	25.405567892016638
37	24.277484097899702	23.842736766607334	26.135238278517924	25.74454085697504
38	24.01036677123444	23.75787749550198	26.503135831439767	25.72861990182382
39	23.720847392630727	23.555264486349458	27.180518187722896	25.543369933296916
40	23.916490321990334	23.38675596988163	27.469880097223083	25.22687361090495
41	23.388778204684805	23.373155612045522	27.868641594436006	25.369424588833667
42	22.939477413087623	23.579962272060364	27.91173840171678	25.56882191313524
43	22.952505909442504	22.748616571766668	27.85388093794234	26.44499658084849
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	820.0
1	1001.0
2	1182.0
3	2630.0
4	4078.0
5	4078.0
6	4842.0
7	5606.0
8	6078.5
9	6551.0
10	9924.5
11	13298.0
12	13298.0
13	22776.0
14	32254.0
15	52013.5
16	71773.0
17	58296.0
18	44819.0
19	44819.0
20	53246.0
21	61673.0
22	59919.0
23	58165.0
24	71485.5
25	84806.0
26	84806.0
27	104201.5
28	123597.0
29	145540.5
30	167484.0
31	200446.0
32	233408.0
33	233408.0
34	272084.0
35	310760.0
36	361380.5
37	412001.0
38	469890.5
39	527780.0
40	527780.0
41	590814.5
42	653849.0
43	722069.0
44	790289.0
45	858351.5
46	926414.0
47	926414.0
48	987520.0
49	1048626.0
50	1078682.0
51	1108738.0
52	1104060.0
53	1099382.0
54	1099382.0
55	1057207.5
56	1015033.0
57	949370.0
58	883707.0
59	807646.0
60	731585.0
61	731585.0
62	641696.0
63	551807.0
64	472170.5
65	392534.0
66	326086.0
67	259638.0
68	259638.0
69	212153.0
70	164668.0
71	135313.0
72	105958.0
73	86380.5
74	66803.0
75	66803.0
76	54846.0
77	42889.0
78	34910.0
79	26931.0
80	21531.5
81	16132.0
82	16132.0
83	12775.5
84	9419.0
85	7406.5
86	5394.0
87	4357.0
88	3320.0
89	3320.0
90	2448.0
91	1576.0
92	1134.0
93	692.0
94	487.0
95	282.0
96	282.0
97	188.0
98	94.0
99	69.0
100	44.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1.2065859E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.4996788016144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.77269935063556	40.56768714893871
2	10.593873039894525	10.911621176393007
3	3.6733619295320605	5.675308784789388
4	1.8791232752380391	3.870969804135867
5	1.1688942026372102	3.0098837994442755
6	0.745943252947662	2.3049502758621596
7	0.5210249563372158	1.8782832529294247
8	0.38481952353252985	1.5854465486812463
9	0.2944721800816323	1.3648700421193696
>10	1.7560299933597472	16.89726508629864
>50	0.1462257410041047	5.172471634443033
>100	0.062011197716104555	5.4129394591269095
>500	0.0011826264064140988	0.4013243758234427
>1k	2.7421137625966124E-4	0.2259891938116528
>5k	0.0	0.0
>10k+	6.451930099254756E-5	0.7209894172027608
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	43405	0.35973402308115815	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16945	0.1404375768024473	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13644	0.1130793920267094	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12801	0.10609273653869153	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.315139021598048E-5
2	0.0	0.0	0.0	0.0	3.315139021598048E-5
3	0.0	0.0	0.0	8.28784755399512E-6	3.315139021598048E-5
4	0.0	0.0	0.0	8.28784755399512E-6	4.14392377699756E-5
5	0.0	8.28784755399512E-6	0.0	8.28784755399512E-6	4.9727085323970715E-5
6	8.28784755399512E-6	8.28784755399512E-6	0.0	8.28784755399512E-6	9.116632309394632E-5
7	8.28784755399512E-6	8.28784755399512E-6	0.0	1.657569510799024E-5	1.0774201820193655E-4
8	8.28784755399512E-6	8.28784755399512E-6	0.0	1.657569510799024E-5	1.4089340841791703E-4
9	1.657569510799024E-5	8.28784755399512E-6	0.0	3.315139021598048E-5	2.154840364038731E-4
10	2.4863542661985357E-5	8.28784755399512E-6	0.0	4.14392377699756E-5	2.154840364038731E-4
11	2.4863542661985357E-5	8.28784755399512E-6	0.0	4.14392377699756E-5	2.154840364038731E-4
12	2.4863542661985357E-5	8.28784755399512E-6	0.0	7.459062798595607E-5	2.154840364038731E-4
13	2.4863542661985357E-5	8.28784755399512E-6	0.0	8.28784755399512E-5	2.154840364038731E-4
14	3.315139021598048E-5	8.28784755399512E-6	0.0	9.116632309394632E-5	2.900746643898292E-4
15	3.315139021598048E-5	1.657569510799024E-5	0.0	1.3260556086392192E-4	2.983625119438243E-4
16	3.315139021598048E-5	1.657569510799024E-5	0.0	1.740447986338975E-4	3.5637744482179014E-4
17	3.315139021598048E-5	1.657569510799024E-5	0.0	3.1493820705181456E-4	3.5637744482179014E-4
18	3.315139021598048E-5	1.657569510799024E-5	0.0	3.7295313992978036E-4	3.646652923757853E-4
19	4.9727085323970715E-5	1.657569510799024E-5	0.0	5.221343959016926E-4	3.646652923757853E-4
20	6.630278043196096E-5	1.657569510799024E-5	0.0	7.459062798595607E-4	3.646652923757853E-4
21	7.459062798595607E-5	1.657569510799024E-5	0.0	0.0012514649806532631	3.812409874837755E-4
22	7.459062798595607E-5	1.657569510799024E-5	0.0	0.0019890834129588286	3.8952883503777063E-4
23	7.459062798595607E-5	1.657569510799024E-5	0.0	0.0023951879431045897	4.7240731057772184E-4
24	7.459062798595607E-5	1.657569510799024E-5	0.0	0.002975337271884248	4.7240731057772184E-4
25	7.459062798595607E-5	2.4863542661985357E-5	0.0	0.003389729649584004	4.88983005685712E-4
26	7.459062798595607E-5	2.4863542661985357E-5	0.0	0.004036181758795623	4.972708532397071E-4
27	7.459062798595607E-5	2.4863542661985357E-5	0.0	0.009091768766732646	5.055587007937023E-4
28	7.459062798595607E-5	2.4863542661985357E-5	0.0	0.041372934989543635	5.055587007937023E-4
29	7.459062798595607E-5	2.4863542661985357E-5	0.0	0.1068137792758891	5.055587007937023E-4
30	8.28784755399512E-5	3.315139021598048E-5	0.0	0.19381960289772987	5.055587007937023E-4
31	8.28784755399512E-5	3.315139021598048E-5	0.0	0.602990636638469	5.055587007937023E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	8395	0.0	21.50804	37
TCTTATA	15265	0.0	16.130692	37
AACGCAG	20535	0.0	15.531531	5
TATACCG	465	0.0	15.118279	5
GGTATCA	12850	0.0	15.102333	1
TATACTG	1545	0.0	13.889967	5
ACGCAGA	23330	0.0	13.631162	6
CGCAGAG	23735	0.0	13.453128	7
AGAGTAC	23665	0.0	13.367844	10
CAGAGTA	24520	0.0	13.188417	9
CTCTTAT	23870	0.0	13.113532	37
TCAACGC	24830	0.0	12.882199	3
CAACGCA	24790	0.0	12.873135	4
ATCAACG	24815	0.0	12.860166	2
GCAGAGT	25685	0.0	12.676661	8
TATCAAC	25085	0.0	12.655373	1
GTATTAG	950	0.0	12.463158	1
GAGTACT	13345	0.0	12.351816	11
TAGATCG	400	0.0	12.025001	5
CTATACT	1370	0.0	11.883212	4
>>END_MODULE