FastQCFastQC Report
Thu 9 Feb 2017
SRR2727370.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727370.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15868531
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT521410.32858113961525487No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT219310.13820434922426028No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT175930.11086722520187912No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158850.1001037840238646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC110600.020.89195337
GGTATCA133450.016.7463471
TATACCG6600.016.5378785
AACGCAG263350.016.164235
TCTTATA193050.015.68738737
TCGTTAC26050.014.8426123
TAGATCG4850.014.4948455
CTCGTTA26250.014.44761922
ACGCAGA295750.014.4246836
TCAACGC297850.014.267083
CAACGCA298050.014.25134
ATCAACG298150.014.2279062
CGCAGAG301100.014.1991037
CGTTACG22300.014.18609824
TACTTAC34550.014.0824931
AGAGTAC302900.014.04143210
CAGAGTA312400.013.8217039
TATCAAC305550.013.7803961
GTACTTA38500.013.40649330
GCAGAGT326200.013.3674138
Produced by FastQC (version 0.11.5)