FastQCFastQC Report
Thu 9 Feb 2017
SRR2727369.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727369.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9675246
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT165490.17104474656251634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC50750.019.97635337
GGTATCA68500.017.7437951
TATACTG11900.014.9243715
TCTTATA87450.014.91423637
AACGCAG137400.014.8780935
ACGCAGA148900.013.8408346
TCAACGC148300.013.7596093
CAACGCA149100.013.7230044
AGAGTAC149600.013.62767310
ATCAACG151450.013.6200062
TATCAAC151100.013.4556591
CGCAGAG154800.013.3730627
TCGTTAC10550.013.15165923
CAGAGTA159550.013.1372619
TCTATAC8350.013.07185653
CTCTTAT135400.013.03471337
GTATCAA93600.012.8274562
TAGACAG19500.012.712825
GCAGAGT168550.012.5784648
GTACTAA4300.012.0465121
Produced by FastQC (version 0.11.5)