FastQCFastQC Report
Thu 9 Feb 2017
SRR2727368.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727368.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11037368
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC43850.019.66020437
GGTATCA48050.018.519251
TCTTATA79200.016.95833437
AACGCAG103050.014.6851035
TCAACGC103350.014.5887753
CAACGCA104500.014.375124
TATACTG12400.014.1733885
ATCAACG106700.014.1654182
GTATCAA64650.013.7641142
ACGCAGA110000.013.7236366
TATCAAC109600.013.5711691
AGAGTAC112650.013.46648910
TAGACAG22050.013.3401375
TTAGACA16500.013.2303044
GTCTTAG16300.013.1656441
TTATACT11950.013.0041854
CAGAGTA118950.012.9554429
CTCTTAT129800.012.92719637
CGCAGAG118450.012.9008037
GTATAAG11450.012.2794751
Produced by FastQC (version 0.11.5)