##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727368.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11037368
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.92058025065396	31.0	31.0	34.0	30.0	34.0
2	32.076041588900544	33.0	31.0	34.0	30.0	34.0
3	32.15905621702565	34.0	31.0	34.0	30.0	34.0
4	35.798681261692096	37.0	35.0	37.0	35.0	37.0
5	35.63912682806263	37.0	35.0	37.0	33.0	37.0
6	35.647241624996106	37.0	35.0	37.0	33.0	37.0
7	35.593706669923485	37.0	35.0	37.0	33.0	37.0
8	35.551171982305924	37.0	35.0	37.0	33.0	37.0
9	37.255586295573366	39.0	37.0	39.0	34.0	39.0
10	37.04973404891456	39.0	37.0	39.0	33.0	39.0
11	37.17232813112691	39.0	37.0	39.0	34.0	39.0
12	37.05316575473428	39.0	37.0	39.0	33.0	39.0
13	37.12130428196287	39.0	37.0	39.0	33.0	39.0
14	38.25959359151566	40.0	38.0	41.0	34.0	41.0
15	38.202036844291136	40.0	38.0	41.0	33.0	41.0
16	38.131399261128195	40.0	38.0	41.0	33.0	41.0
17	38.06142605737165	40.0	37.0	41.0	33.0	41.0
18	37.99184071782331	40.0	37.0	41.0	33.0	41.0
19	37.98181921632041	40.0	37.0	41.0	33.0	41.0
20	37.853009974841825	40.0	37.0	41.0	33.0	41.0
21	37.7590036863861	40.0	37.0	41.0	32.0	41.0
22	37.67832086417704	39.0	37.0	41.0	32.0	41.0
23	37.558188147754066	39.0	37.0	40.0	32.0	41.0
24	37.43328590656758	39.0	37.0	41.0	32.0	41.0
25	37.3435296349637	39.0	37.0	40.0	32.0	41.0
26	36.953299192343685	39.0	36.0	40.0	31.0	41.0
27	36.72111566815567	39.0	36.0	40.0	30.0	41.0
28	36.56684736795946	39.0	36.0	40.0	30.0	41.0
29	36.53493930799444	39.0	36.0	40.0	30.0	41.0
30	36.46454109349258	39.0	36.0	40.0	30.0	41.0
31	36.25771143990125	39.0	35.0	40.0	29.0	41.0
32	36.1590701696274	38.0	35.0	40.0	29.0	41.0
33	35.98193826644178	38.0	35.0	40.0	28.0	41.0
34	35.84365928543834	38.0	35.0	40.0	27.0	41.0
35	35.688037582873015	38.0	35.0	40.0	27.0	41.0
36	35.542879878608744	38.0	34.0	40.0	26.0	41.0
37	35.29048048411541	38.0	34.0	40.0	25.0	41.0
38	35.1936791452455	38.0	34.0	40.0	25.0	41.0
39	35.02773269859264	38.0	34.0	40.0	25.0	41.0
40	34.90554994632778	38.0	33.0	40.0	24.0	41.0
41	34.786330309907214	38.0	33.0	40.0	24.0	41.0
42	34.59945858469157	38.0	33.0	40.0	24.0	41.0
43	31.064911217964283	35.0	27.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	10.0
11	20.0
12	13.0
13	39.0
14	64.0
15	164.0
16	299.0
17	699.0
18	1397.0
19	3047.0
20	6433.0
21	12196.0
22	21599.0
23	34931.0
24	51542.0
25	72348.0
26	97630.0
27	128545.0
28	165615.0
29	208287.0
30	259963.0
31	323030.0
32	398370.0
33	493289.0
34	628105.0
35	822308.0
36	1146773.0
37	1768435.0
38	2963186.0
39	1429027.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.76518641038334	18.092012516027374	11.79308327854974	27.349717795039542
2	19.398338444455234	20.256350970629956	33.50546072215768	26.839849862757138
3	18.78774903582086	21.150558720158646	29.923746313432698	30.1379459305878
4	15.235117647613091	15.232689532504489	32.78002509293883	36.7521677269436
5	14.90856334589913	36.46128316098548	33.893542373507884	14.7366111196075
6	37.88718469838099	34.706498868208435	13.460437307155113	13.945879126255461
7	31.861826116516184	30.412766884278934	18.9581338594491	18.767273139755783
8	28.97187989020571	34.63265880054013	17.843194138312686	18.552267170941477
9	27.72499748128358	12.966234341375587	16.649449397718733	42.6593187796221
10	15.704033787765345	27.07199759942769	33.216904609867136	24.00706400293983
11	39.38158082615348	19.893492723989993	20.18626179719658	20.538664652659946
12	22.800879702479797	23.84270416642808	28.887484769919787	24.468931361172338
13	32.80588270681923	18.660218631833242	22.437831193088787	26.096067468258738
14	23.43180004508321	18.677949308204635	23.86567159851878	34.024579048193374
15	26.680409677379608	26.809018236956494	20.124426403106245	26.386145682557654
16	26.690656685543146	25.141011878918963	22.12475836630617	26.043573069231723
17	26.052388576696906	25.10486195622	23.31715314738079	25.525596319702302
18	26.125458533230024	24.563664090931823	23.51476366467078	25.796113711167372
19	26.898713533878727	24.489615640250463	23.517744447770518	25.093926378100285
20	26.73429027644997	24.220049562540634	23.223688835961617	25.821971325047784
21	26.47643894812604	24.261481541613904	23.2954178931064	25.96666161715365
22	27.037206696379062	24.01023504879062	22.88705060844216	26.06550764638816
23	26.608925243771886	23.91889080802597	23.009063392649406	26.463120555552738
24	26.46156221302035	23.797503172857876	23.142129536679395	26.59880507744238
25	26.63099572289336	23.383246803042173	23.296178944110586	26.689578529953877
26	26.282416242712937	23.704401266678797	23.386961456753095	26.626221033855174
27	26.5254814372412	24.063164334105743	23.094536668524597	26.316817560128463
28	26.542586964573438	24.110594119902498	23.082749438090673	26.264069477433388
29	26.44664017725965	24.38741736254513	23.099864025553916	26.066078434641305
30	26.633931205338087	24.247574240525456	22.88588185154287	26.23261270259359
31	26.477091277558202	23.75504739898135	23.160503482351952	26.6073578411085
32	26.08003103638476	24.13777451290924	23.247761604034586	26.534432846671418
33	26.14332511156645	23.814219114557023	23.345855642395904	26.69660013148062
34	26.368813651950358	23.60201272622241	23.680935527382978	26.348238094444255
35	26.195882931510482	23.659263694025604	23.716632443531825	26.428220930932085
36	26.06390400320076	23.440117245343274	23.90113295126157	26.594845800194395
37	25.899063979745897	23.050051425303568	24.207365379137492	26.843519215813043
38	25.49592439066995	23.124208597556954	24.49689092544527	26.882976086327826
39	25.35869964650993	22.89295781385562	25.029372944709284	26.71896959492517
40	25.526592934112553	22.5855113284254	25.39961519811607	26.488280539345975
41	25.09580182521775	22.511027991455933	25.706581496603174	26.68658868672314
42	24.655751262438656	22.488676648273394	25.9506976663277	26.904874422960255
43	24.82667969392703	21.510961671296997	25.955834760605974	27.706523874170003
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	76.0
1	108.0
2	140.0
3	363.0
4	586.0
5	586.0
6	664.0
7	742.0
8	840.5
9	939.0
10	1411.5
11	1884.0
12	1884.0
13	3327.5
14	4771.0
15	7979.0
16	11187.0
17	9959.0
18	8731.0
19	8731.0
20	11500.0
21	14269.0
22	16776.5
23	19284.0
24	25929.5
25	32575.0
26	32575.0
27	43194.5
28	53814.0
29	68572.5
30	83331.0
31	107400.0
32	131469.0
33	131469.0
34	164058.0
35	196647.0
36	241231.0
37	285815.0
38	343433.0
39	401051.0
40	401051.0
41	466991.0
42	532931.0
43	606390.0
44	679849.0
45	759231.0
46	838613.0
47	838613.0
48	909770.0
49	980927.0
50	1026207.5
51	1071488.0
52	1080160.5
53	1088833.0
54	1088833.0
55	1067412.0
56	1045991.0
57	989352.0
58	932713.0
59	862015.5
60	791318.0
61	791318.0
62	700395.0
63	609472.0
64	521859.5
65	434247.0
66	361340.5
67	288434.0
68	288434.0
69	236814.0
70	185194.0
71	151820.0
72	118446.0
73	96308.5
74	74171.0
75	74171.0
76	60655.0
77	47139.0
78	38577.5
79	30016.0
80	23962.0
81	17908.0
82	17908.0
83	14151.5
84	10395.0
85	8113.5
86	5832.0
87	4609.5
88	3387.0
89	3387.0
90	2530.0
91	1673.0
92	1178.5
93	684.0
94	474.5
95	265.0
96	265.0
97	183.0
98	101.0
99	65.5
100	30.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1.1037368E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.13435979326435
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.76746632078967	40.27723962851091
2	10.294481500120915	10.528034418245733
3	3.773313565057413	5.788379203453523
4	1.9428041304358694	3.9737618165419137
5	1.1549284292411268	2.9528262918142714
6	0.7345859904386095	2.2537550600507585
7	0.5264017040389731	1.8842049891081414
8	0.4042130231719403	1.6535339327997678
9	0.31246473686885173	1.4379915849983584
>10	1.8669304109753866	17.922671515641365
>50	0.15561329106165217	5.4914603242762805
>100	0.06596188852435664	5.447364299640847
>500	6.932420048349013E-4	0.22419406230649214
>1k	1.2404649909786175E-4	0.10725137606961067
>5k	1.772077131476196E-5	0.057331496542041616
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.4360786013477126E-5
2	0.0	0.0	0.0	0.0	5.4360786013477126E-5
3	0.0	0.0	0.0	0.0	5.4360786013477126E-5
4	0.0	0.0	0.0	0.0	6.342091701572331E-5
5	0.0	1.8120262004492374E-5	0.0	0.0	6.342091701572331E-5
6	0.0	1.8120262004492374E-5	0.0	0.0	1.1778170302920044E-4
7	9.060131002246187E-6	1.8120262004492374E-5	0.0	0.0	1.1778170302920044E-4
8	9.060131002246187E-6	1.8120262004492374E-5	0.0	0.0	1.2684183403144662E-4
9	9.060131002246187E-6	1.8120262004492374E-5	0.0	9.060131002246187E-6	2.174431440539085E-4
10	9.060131002246187E-6	1.8120262004492374E-5	0.0	2.7180393006738563E-5	2.174431440539085E-4
11	1.8120262004492374E-5	1.8120262004492374E-5	0.0	3.624052400898475E-5	2.265032750561547E-4
12	1.8120262004492374E-5	1.8120262004492374E-5	0.0	3.624052400898475E-5	2.3556340605840087E-4
13	1.8120262004492374E-5	1.8120262004492374E-5	0.0	3.624052400898475E-5	2.3556340605840087E-4
14	1.8120262004492374E-5	1.8120262004492374E-5	0.0	3.624052400898475E-5	2.808640610696318E-4
15	1.8120262004492374E-5	1.8120262004492374E-5	0.0	4.530065501123094E-5	3.0804445407637036E-4
16	1.8120262004492374E-5	1.8120262004492374E-5	0.0	5.4360786013477126E-5	3.9864576409883226E-4
17	1.8120262004492374E-5	2.7180393006738563E-5	0.0	9.966144102470807E-5	4.1676602610332463E-4
18	1.8120262004492374E-5	2.7180393006738563E-5	0.0	1.2684183403144662E-4	4.530065501123094E-4
19	1.8120262004492374E-5	2.7180393006738563E-5	0.0	1.630823580404314E-4	4.530065501123094E-4
20	1.8120262004492374E-5	2.7180393006738563E-5	0.0	2.174431440539085E-4	4.530065501123094E-4
21	1.8120262004492374E-5	2.7180393006738563E-5	0.0	3.3522484708310897E-4	4.8018694311904793E-4
22	1.8120262004492374E-5	2.7180393006738563E-5	0.0	5.617281221392636E-4	5.164274671280327E-4
23	1.8120262004492374E-5	2.7180393006738563E-5	0.0	6.523294321617256E-4	6.704496941662179E-4
24	1.8120262004492374E-5	2.7180393006738563E-5	0.0	8.878928382201264E-4	6.704496941662179E-4
25	1.8120262004492374E-5	2.7180393006738563E-5	0.0	9.784941482425882E-4	6.885699561707103E-4
26	1.8120262004492374E-5	2.7180393006738563E-5	0.0	0.001250298078309974	7.066902181752027E-4
27	1.8120262004492374E-5	2.7180393006738563E-5	0.0	0.003578751745887244	7.157503491774488E-4
28	1.8120262004492374E-5	2.7180393006738563E-5	0.0	0.02115540589024485	7.24810480179695E-4
29	1.8120262004492374E-5	2.7180393006738563E-5	0.0	0.05701540439713526	7.24810480179695E-4
30	1.8120262004492374E-5	2.7180393006738563E-5	0.0	0.1062209758703343	7.24810480179695E-4
31	1.8120262004492374E-5	2.7180393006738563E-5	0.0	0.3131271875686305	7.24810480179695E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	4385	0.0	19.660204	37
GGTATCA	4805	0.0	18.51925	1
TCTTATA	7920	0.0	16.958334	37
AACGCAG	10305	0.0	14.685103	5
TCAACGC	10335	0.0	14.588775	3
CAACGCA	10450	0.0	14.37512	4
TATACTG	1240	0.0	14.173388	5
ATCAACG	10670	0.0	14.165418	2
GTATCAA	6465	0.0	13.764114	2
ACGCAGA	11000	0.0	13.723636	6
TATCAAC	10960	0.0	13.571169	1
AGAGTAC	11265	0.0	13.466489	10
TAGACAG	2205	0.0	13.340137	5
TTAGACA	1650	0.0	13.230304	4
GTCTTAG	1630	0.0	13.165644	1
TTATACT	1195	0.0	13.004185	4
CAGAGTA	11895	0.0	12.955442	9
CTCTTAT	12980	0.0	12.927196	37
CGCAGAG	11845	0.0	12.900803	7
GTATAAG	1145	0.0	12.279475	1
>>END_MODULE