FastQCFastQC Report
Thu 9 Feb 2017
SRR2727366.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727366.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5297595
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT676971.2778817557778577No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238830.4508272149909534No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT230840.435744899336397No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT176710.3335664579870677No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57760.10903060728500386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC48150.017.09761237
AACGCAG260050.015.6010385
TAGTACT4500.014.3888894
TATTCCG1551.212702E-714.322585
CGCAGAG291800.013.8147717
TATACAC17850.013.78431337
TCTTATA75100.013.52396837
ATACCCG3700.013.5000015
ACGCAGA300650.013.4696486
GAGTACT190700.013.46512911
AGAGTAC300600.013.35495810
AGTACTT192700.013.31577712
CAGAGTA306150.013.2155819
GCAGAGT308050.013.1821138
ACCCGTT5250.013.03809457
GTACTTT202600.012.655972513
TACTTTT206550.012.3960314
CATACGT1359.9731755E-512.3333344
TACCCGT4050.012.3333336
TATACTG6750.012.3333335
Produced by FastQC (version 0.11.5)