FastQCFastQC Report
Thu 9 Feb 2017
SRR2727365.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727365.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5283556
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT366430.6935291307596626No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT144230.27297903154617836No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124840.23628026276242745No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT104690.19814306879684818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC37150.019.5208637
TCTTATA62000.014.68064437
CTCGTTA7150.014.23076922
AACGCAG175450.013.7076095
TTTACGC950.001246111713.6315793
TACTTAC8850.013.5875731
GAGTACT108000.013.583796511
TATACAC12350.013.331984537
CTCTTAT97150.013.29181837
AGTACTT110650.013.24175312
TAAGACT8300.012.9277114
TCGTTAC6600.012.8939423
TATACCG2451.1459633E-1012.8367345
GTACTTA9200.012.66847930
TAGTACT3800.012.6578954
GTACTTT117300.012.45950513
ATACCGT2552.237357E-1012.3333336
TAGACAG9550.012.2041885
CGCAGAG198050.012.1714217
GTATTAG5800.012.1206891
Produced by FastQC (version 0.11.5)