##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727365.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5283556
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07688912542992	33.0	31.0	34.0	30.0	34.0
2	32.22572676432312	33.0	31.0	34.0	30.0	34.0
3	32.30276408540006	34.0	31.0	34.0	30.0	34.0
4	35.88772315463298	37.0	35.0	37.0	35.0	37.0
5	35.769374262333926	37.0	35.0	37.0	35.0	37.0
6	35.79064667810846	37.0	35.0	37.0	35.0	37.0
7	35.73674245148533	37.0	35.0	37.0	35.0	37.0
8	35.71605335497532	37.0	35.0	37.0	33.0	37.0
9	37.42955729058233	39.0	37.0	39.0	35.0	39.0
10	37.2686696232613	39.0	37.0	39.0	34.0	39.0
11	37.37573974800305	39.0	37.0	39.0	34.0	39.0
12	37.28252071142995	39.0	37.0	39.0	34.0	39.0
13	37.343260864463254	39.0	37.0	39.0	34.0	39.0
14	38.48572968659744	40.0	38.0	41.0	34.0	41.0
15	38.455417714887474	40.0	38.0	41.0	34.0	41.0
16	38.3847798717379	40.0	38.0	41.0	34.0	41.0
17	38.35849870806707	40.0	38.0	41.0	34.0	41.0
18	38.31548733466627	40.0	38.0	41.0	34.0	41.0
19	38.33175289520921	40.0	38.0	41.0	34.0	41.0
20	38.21830808644784	40.0	38.0	41.0	34.0	41.0
21	38.14046695066732	40.0	38.0	41.0	33.0	41.0
22	38.072642742879985	40.0	38.0	41.0	33.0	41.0
23	37.95700887811164	40.0	37.0	41.0	33.0	41.0
24	37.854476606285616	40.0	37.0	41.0	33.0	41.0
25	37.770895775496655	40.0	37.0	41.0	33.0	41.0
26	37.40998164872294	39.0	37.0	41.0	32.0	41.0
27	37.19377006697762	39.0	36.0	41.0	31.0	41.0
28	37.0423559436107	39.0	36.0	40.0	31.0	41.0
29	36.99491687038048	39.0	36.0	40.0	31.0	41.0
30	36.91525082728375	39.0	36.0	40.0	30.0	41.0
31	36.69978306277061	39.0	36.0	40.0	30.0	41.0
32	36.58900823611977	39.0	36.0	40.0	30.0	41.0
33	36.40655668265842	39.0	35.0	40.0	30.0	41.0
34	36.27016577471688	39.0	35.0	40.0	30.0	41.0
35	36.11299851842206	39.0	35.0	40.0	29.0	41.0
36	35.95946101451371	38.0	35.0	40.0	28.0	41.0
37	35.72361833583291	38.0	35.0	40.0	27.0	41.0
38	35.61866932043495	38.0	35.0	40.0	27.0	41.0
39	35.44064660240187	38.0	34.0	40.0	26.0	41.0
40	35.29838938018259	38.0	34.0	40.0	25.0	41.0
41	35.15583406327102	38.0	34.0	40.0	25.0	41.0
42	34.9514616671045	38.0	34.0	40.0	24.0	41.0
43	31.58533854850786	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	25.0
11	32.0
12	29.0
13	21.0
14	29.0
15	67.0
16	120.0
17	218.0
18	481.0
19	1063.0
20	2138.0
21	4206.0
22	7633.0
23	12656.0
24	19379.0
25	28153.0
26	39020.0
27	51910.0
28	68589.0
29	87622.0
30	109822.0
31	137804.0
32	171632.0
33	215627.0
34	277665.0
35	364636.0
36	511154.0
37	806770.0
38	1411531.0
39	953518.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.10232653917173	18.862996815023823	13.044964414118068	25.989712231686386
2	18.530777377962874	21.605941150240483	34.49636949054765	25.36691198124899
3	18.823099442875215	22.322844690204853	30.983829829758598	27.870226037161338
4	14.937439860578747	15.758761712755575	33.13298846458711	36.17080996207857
5	14.576716892941041	37.27871910508756	33.74269147521101	14.401872526760387
6	36.140167720376205	35.183955654108715	13.998261776727643	14.677614848787446
7	29.770764235299108	31.14839702654803	20.098755459391366	18.9820832787615
8	28.248077620451074	35.22847112815687	18.5759174313663	17.947533820025754
9	27.06107401908866	13.756189959943644	18.225168806765744	40.95756721420195
10	15.808614501294205	28.130940601367715	33.330525880675815	22.729919016662265
11	37.19474157177476	21.283147183449934	20.83666757766928	20.685443667106018
12	21.59873009768421	25.363202358411645	29.834149576535197	23.203917967368945
13	32.118709444926864	19.61612595759371	23.623143201283376	24.642021396196046
14	22.399630097608505	20.35364061628191	25.921992688257685	31.3247365978519
15	25.83405948569486	28.187720542755674	21.659806387970526	24.318413583578938
16	24.694940301569623	26.205722055373315	24.142925711395886	24.956411931661176
17	24.319833082113636	26.457446462193264	25.30405658613252	23.91866386956058
18	24.241060376761407	25.6727665988588	25.923468966733772	24.162704057646025
19	25.316377833413707	25.67971267835526	25.93948091020517	23.06442857802586
20	24.721967553670293	25.437224475334414	25.686318078203392	24.1544898927919
21	24.407028145438414	25.80765681294946	25.88315520834832	23.90215983326381
22	25.10188214149713	25.25062666128645	25.106651656573714	24.540839540642704
23	24.454723296204296	25.313841662698376	25.526198643489344	24.705236397607973
24	24.283228946565533	25.272770838427753	25.62149431178547	24.82250590322124
25	24.672417591485736	24.943731078084532	25.542097027077975	24.841754303351756
26	24.228985175892902	25.235750316642807	26.020278766800235	24.514985740664052
27	24.78991421686455	25.646061099759326	25.565774262636754	23.998250420739364
28	24.517351571555217	25.835819663877892	25.358300356805152	24.288528407761742
29	24.272383977760434	25.940256902737474	25.818312515283264	23.969046604218825
30	24.663219240980887	25.684065807195	25.44348919553422	24.209225756289893
31	24.383748369469348	25.175279679064634	25.729149080657045	24.711822870808977
32	24.000464838453496	25.67515135639709	25.635197204307097	24.689186600842312
33	24.228985175892902	25.13280828290644	25.6515309007797	24.98667564042096
34	24.421450250550954	25.077996712819928	26.155282540773676	24.34527049585544
35	24.189333850156977	25.083561147075944	26.089644928529193	24.63746007423788
36	23.958826214768994	24.708302514442927	26.54004235026562	24.792828920522467
37	23.54577106781872	24.38757155218947	26.93284598478752	25.13381139520429
38	23.50117988718204	24.254782195930165	27.181674614596684	25.062363302291107
39	23.07497072047689	24.180551885888974	27.87009355063143	24.874383843002704
40	23.470140185889957	23.922259932515146	28.102569557320866	24.50503032427403
41	22.946326299938903	23.96533698138148	28.31592965040969	24.77240706826993
42	22.47929235537581	24.222322996103383	28.36678555124617	24.93159909727464
43	22.519076167641643	23.46143392821047	28.189348234408797	25.830141669739092
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1233.0
1	1181.0
2	1129.0
3	2351.0
4	3573.0
5	3573.0
6	4059.0
7	4545.0
8	4838.0
9	5131.0
10	7870.0
11	10609.0
12	10609.0
13	19207.0
14	27805.0
15	43029.5
16	58254.0
17	44493.5
18	30733.0
19	30733.0
20	35687.0
21	40641.0
22	35931.0
23	31221.0
24	38044.5
25	44868.0
26	44868.0
27	54200.5
28	63533.0
29	72544.0
30	81555.0
31	94992.0
32	108429.0
33	108429.0
34	124336.5
35	140244.0
36	161072.0
37	181900.0
38	206536.5
39	231173.0
40	231173.0
41	258493.5
42	285814.0
43	314168.0
44	342522.0
45	372865.0
46	403208.0
47	403208.0
48	431829.0
49	460450.0
50	475068.5
51	489687.0
52	484395.0
53	479103.0
54	479103.0
55	451699.5
56	424296.0
57	393576.5
58	362857.0
59	333323.0
60	303789.0
61	303789.0
62	265143.0
63	226497.0
64	191492.5
65	156488.0
66	129775.0
67	103062.0
68	103062.0
69	84333.0
70	65604.0
71	53766.5
72	41929.0
73	34425.0
74	26921.0
75	26921.0
76	21979.5
77	17038.0
78	14118.5
79	11199.0
80	9095.5
81	6992.0
82	6992.0
83	5565.0
84	4138.0
85	3256.0
86	2374.0
87	1996.0
88	1618.0
89	1618.0
90	1219.5
91	821.0
92	603.5
93	386.0
94	255.5
95	125.0
96	125.0
97	79.5
98	34.0
99	31.0
100	28.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5283556.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.48299929769832
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.25921134211596	46.71023188510372
2	9.70553888471529	11.158069697877458
3	3.3110311508928354	5.709840039642904
4	1.6172929134369745	3.7186738962908037
5	0.9468176315729321	2.7212958625377626
6	0.6104364035114406	2.105382921260258
7	0.4296802608076198	1.728951709116747
8	0.3152368580012187	1.4496608069674144
9	0.23667191300712265	1.2244150268256018
>10	1.4526006260989597	15.353203547867702
>50	0.0865846793256087	3.3270396280483685
>100	0.02731789633124461	2.616075090299818
>500	0.0010529623893058481	0.4051171496615032
>1k	3.948583451475743E-4	0.3719954372954284
>5k	0.0	0.0
>10k+	1.3161944837971698E-4	1.4000473012046135
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	36643	0.6935291307596626	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14423	0.27297903154617836	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12484	0.23628026276242745	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10469	0.19814306879684818	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.677994138795917E-5
2	0.0	0.0	0.0	0.0	5.677994138795917E-5
3	0.0	0.0	0.0	0.0	5.677994138795917E-5
4	0.0	0.0	0.0	0.0	5.677994138795917E-5
5	0.0	0.0	0.0	0.0	5.677994138795917E-5
6	0.0	0.0	0.0	0.0	5.677994138795917E-5
7	0.0	0.0	0.0	0.0	5.677994138795917E-5
8	1.8926647129319723E-5	0.0	0.0	0.0	7.570658851727889E-5
9	1.8926647129319723E-5	0.0	0.0	0.0	1.3248652990523806E-4
10	1.8926647129319723E-5	0.0	0.0	0.0	1.3248652990523806E-4
11	3.7853294258639446E-5	0.0	0.0	1.8926647129319723E-5	1.3248652990523806E-4
12	3.7853294258639446E-5	0.0	0.0	7.570658851727889E-5	1.3248652990523806E-4
13	5.677994138795917E-5	0.0	0.0	7.570658851727889E-5	1.3248652990523806E-4
14	7.570658851727889E-5	0.0	0.0	9.463323564659862E-5	2.0819311842251695E-4
15	7.570658851727889E-5	1.8926647129319723E-5	0.0	1.8926647129319723E-4	2.0819311842251695E-4
16	9.463323564659862E-5	1.8926647129319723E-5	0.0	3.7853294258639446E-4	2.649730598104761E-4
17	9.463323564659862E-5	1.8926647129319723E-5	0.0	7.002859437848297E-4	2.838997069397959E-4
18	9.463323564659862E-5	1.8926647129319723E-5	0.0	7.570658851727889E-4	3.7853294258639446E-4
19	9.463323564659862E-5	1.8926647129319723E-5	0.0	0.0011355988277591835	3.974595897157142E-4
20	9.463323564659862E-5	1.8926647129319723E-5	0.0	0.0016466183002508158	3.974595897157142E-4
21	1.1355988277591834E-4	1.8926647129319723E-5	0.0	0.0023469042440356458	4.163862368450339E-4
22	1.1355988277591834E-4	1.8926647129319723E-5	0.0	0.0037474761316053052	4.3531288397435366E-4
23	1.1355988277591834E-4	1.8926647129319723E-5	0.0	0.004637028546683332	5.488727667502719E-4
24	1.1355988277591834E-4	1.8926647129319723E-5	0.0	0.005980820492865032	5.488727667502719E-4
25	1.1355988277591834E-4	1.8926647129319723E-5	0.0	0.006908226202201699	5.677994138795917E-4
26	1.1355988277591834E-4	1.8926647129319723E-5	0.0	0.00821416485412476	5.677994138795917E-4
27	1.1355988277591834E-4	1.8926647129319723E-5	0.0	0.01440317846541231	5.677994138795917E-4
28	1.1355988277591834E-4	1.8926647129319723E-5	0.0	0.04850899659244645	5.677994138795917E-4
29	1.1355988277591834E-4	1.8926647129319723E-5	0.0	0.12031669580108548	5.677994138795917E-4
30	1.3248652990523806E-4	1.8926647129319723E-5	0.0	0.21519597786036526	5.677994138795917E-4
31	1.3248652990523806E-4	1.8926647129319723E-5	0.0	0.5895650580783094	5.677994138795917E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	3715	0.0	19.52086	37
TCTTATA	6200	0.0	14.680644	37
CTCGTTA	715	0.0	14.230769	22
AACGCAG	17545	0.0	13.707609	5
TTTACGC	95	0.0012461117	13.631579	3
TACTTAC	885	0.0	13.58757	31
GAGTACT	10800	0.0	13.5837965	11
TATACAC	1235	0.0	13.3319845	37
CTCTTAT	9715	0.0	13.291818	37
AGTACTT	11065	0.0	13.241753	12
TAAGACT	830	0.0	12.927711	4
TCGTTAC	660	0.0	12.89394	23
TATACCG	245	1.1459633E-10	12.836734	5
GTACTTA	920	0.0	12.668479	30
TAGTACT	380	0.0	12.657895	4
GTACTTT	11730	0.0	12.459505	13
ATACCGT	255	2.237357E-10	12.333333	6
TAGACAG	955	0.0	12.204188	5
CGCAGAG	19805	0.0	12.171421	7
GTATTAG	580	0.0	12.120689	1
>>END_MODULE