FastQCFastQC Report
Thu 9 Feb 2017
SRR2727364.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727364.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936950
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT78770.8407065478414003No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33330.3557286941672448No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29810.3181599871924862No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23540.2512407278936976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC6350.019.22834637
CCGACAT701.2188923E-418.514
CGGTAGT701.2188923E-418.51
GGTCTAC1552.0008883E-1117.9032251
GTTCTAA852.7222462E-517.4117641
TAGGAGT1301.3920726E-817.0769234
CGACGTC700.002592014415.85714236
AACGCAG30400.015.5180925
TCTTATA10850.015.516129537
GTACTTA2750.015.47272830
TACTTAC2650.015.35849131
AGTGTAC3200.015.0312527
TTACCCT1001.0930213E-414.7999994
GGAGTGT3650.014.698636
TTATGAC1405.990896E-714.5357143
GTCTAAA1405.990896E-714.5357141
TTAGGCT800.006297476613.8754
CGACCGA800.006297476613.87518
CGACAAA800.006297476613.87514
CATTACG1601.781791E-713.87524
Produced by FastQC (version 0.11.5)