##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727364.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	936950
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09028443353434	33.0	31.0	34.0	30.0	34.0
2	32.23798388387854	33.0	31.0	34.0	30.0	34.0
3	32.319690485084585	34.0	31.0	34.0	30.0	34.0
4	35.89170393297401	37.0	35.0	37.0	35.0	37.0
5	35.78352099898607	37.0	35.0	37.0	35.0	37.0
6	35.813420139815356	37.0	35.0	37.0	35.0	37.0
7	35.75820908266183	37.0	35.0	37.0	35.0	37.0
8	35.74620310582208	37.0	35.0	37.0	35.0	37.0
9	37.46073429745451	39.0	37.0	39.0	35.0	39.0
10	37.30622658626394	39.0	37.0	39.0	34.0	39.0
11	37.422241314904745	39.0	37.0	39.0	35.0	39.0
12	37.33044025828486	39.0	37.0	39.0	34.0	39.0
13	37.39385666257538	39.0	37.0	39.0	34.0	39.0
14	38.545611825604354	40.0	38.0	41.0	34.0	41.0
15	38.52373232296281	40.0	38.0	41.0	34.0	41.0
16	38.46512514008218	40.0	38.0	41.0	34.0	41.0
17	38.44270985644912	40.0	38.0	41.0	34.0	41.0
18	38.39912908906558	40.0	38.0	41.0	34.0	41.0
19	38.42620630770052	40.0	38.0	41.0	34.0	41.0
20	38.31876941138801	40.0	38.0	41.0	34.0	41.0
21	38.25238806766637	40.0	38.0	41.0	34.0	41.0
22	38.18820214525855	40.0	38.0	41.0	34.0	41.0
23	38.08738993542878	40.0	38.0	41.0	33.0	41.0
24	37.99401782378996	40.0	37.0	41.0	33.0	41.0
25	37.91979828165857	40.0	37.0	41.0	33.0	41.0
26	37.58513474571749	40.0	37.0	41.0	32.0	41.0
27	37.38852019851646	39.0	37.0	41.0	32.0	41.0
28	37.241503815571804	39.0	36.0	41.0	31.0	41.0
29	37.200386359997864	39.0	36.0	40.0	31.0	41.0
30	37.12555419179252	39.0	36.0	40.0	31.0	41.0
31	36.909843641603075	39.0	36.0	40.0	31.0	41.0
32	36.81265062169806	39.0	36.0	40.0	30.0	41.0
33	36.64624686482737	39.0	35.0	40.0	30.0	41.0
34	36.51951651635626	39.0	35.0	40.0	30.0	41.0
35	36.36805058967928	39.0	35.0	40.0	30.0	41.0
36	36.22042691712471	39.0	35.0	40.0	30.0	41.0
37	35.995341266876565	38.0	35.0	40.0	28.0	41.0
38	35.898978600779124	38.0	35.0	40.0	28.0	41.0
39	35.738734190725225	38.0	35.0	40.0	27.0	41.0
40	35.60217941192166	38.0	35.0	40.0	27.0	41.0
41	35.456116121457924	38.0	35.0	40.0	26.0	41.0
42	35.25116068093281	38.0	34.0	40.0	25.0	41.0
43	32.029573616521695	35.0	30.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	4.0
13	7.0
14	2.0
15	7.0
16	10.0
17	32.0
18	56.0
19	119.0
20	309.0
21	607.0
22	1075.0
23	1884.0
24	2924.0
25	4222.0
26	5902.0
27	8253.0
28	11109.0
29	14199.0
30	18097.0
31	22921.0
32	29177.0
33	36863.0
34	47869.0
35	63528.0
36	89850.0
37	143281.0
38	255054.0
39	179586.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.25844495437323	20.392443566892577	13.899461017130049	24.44965046160414
2	18.315171567319492	22.56214312396606	35.17231442446235	23.950370884252095
3	18.37878221890176	22.998986071828806	32.09851112652756	26.523720582741877
4	14.522012914243025	16.292651688990876	34.52649554405251	34.65883985271359
5	13.857409680345803	38.358610384759054	34.2910507497732	13.492929185121938
6	35.004856182293615	36.57591120123806	14.38902822989487	14.030204386573455
7	28.904317199423662	32.26276749026095	20.736858957254924	18.09605635306046
8	27.714819360691607	36.050909867122044	19.106569187256525	17.127701584929824
9	26.91947275735098	14.002881690591815	19.045413309141363	40.032232242915846
10	15.223971396552644	28.98489780671327	34.186989700624366	21.604141096109718
11	36.49927957735205	21.873845989647258	21.6076631623886	20.019211270612093
12	20.897913442552966	26.125193446822138	30.876247398473772	22.100645712151127
13	31.3367842467581	20.34921820801537	24.591493676290092	23.722503868936442
14	21.572549228880945	21.021612679438604	27.15491755163029	30.250920540050164
15	24.945301243396127	29.361652169272638	22.469822295746837	23.223224291584398
16	23.750466940605154	27.224291584396177	25.031431773307006	23.99380970169166
17	23.537328566092107	27.27349378301937	26.13010299375634	23.059074657132182
18	23.33464966113453	26.56449116815198	26.94636853620791	23.154490634505574
19	24.408239500506966	26.326591600405568	27.221516623085545	22.04365227600192
20	23.829233150114735	26.164896739420463	26.940498425743105	23.065371684721704
21	23.603073803297935	26.45221196435242	27.194087197822725	22.75062703452692
22	24.141309568280057	25.85580874112813	26.443673621858153	23.559208068733657
23	23.48086877634879	26.12081754629382	26.903997011580127	23.494316665777255
24	23.411814931426438	25.996691392283473	26.897593254709427	23.69390042158066
25	23.74822562570041	25.720689471156412	26.797267730401835	23.733817172741343
26	23.29985591547041	26.16937936922995	27.149901275414912	23.380863439884735
27	23.849084796413898	26.432787235177972	26.737926250066707	22.980201718341426
28	23.466780511233257	26.62223170926944	26.59266769838305	23.318320081114255
29	23.356315705213724	26.765142216767167	26.91402956401089	22.964512514008216
30	23.89455147019585	26.54335877047868	26.391056086237256	23.17103367308821
31	23.500186776242064	26.012167138054327	26.985858370243875	23.501787715459738
32	23.10165964032232	26.521692726399486	26.822989487165806	23.553658146112387
33	23.358663749399646	25.9075724424996	26.752441432306956	23.9813223757938
34	23.41266876567586	25.954106409093335	27.382250920540052	23.250973904690753
35	23.350659053311276	25.879289182987353	27.053418005229734	23.716633758471637
36	23.200917871818135	25.70030417845136	27.40583809168045	23.692939858050053
37	22.774320934948502	25.450771119056515	27.907145525374887	23.867762420620096
38	22.95127808314211	25.308821175089385	27.96520625433588	23.774694487432626
39	22.365227600192114	25.331767970542717	28.567906505149686	23.735097924115482
40	22.741875233470303	25.12578045786862	28.671967554298522	23.460376754362557
41	22.327018517530284	25.115107529750784	28.900048028176528	23.657825924542397
42	21.992955867442234	25.37424622445168	28.731095576071297	23.901702332034795
43	21.97822722663963	24.584769731575857	28.63450557660494	24.80249746517957
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	788.0
1	680.5
2	573.0
3	839.0
4	1105.0
5	1105.0
6	1183.5
7	1262.0
8	1294.0
9	1326.0
10	1967.5
11	2609.0
12	2609.0
13	4545.0
14	6481.0
15	9429.5
16	12378.0
17	9512.5
18	6647.0
19	6647.0
20	7857.5
21	9068.0
22	8072.5
23	7077.0
24	8611.5
25	10146.0
26	10146.0
27	12186.0
28	14226.0
29	16225.5
30	18225.0
31	21142.5
32	24060.0
33	24060.0
34	27399.0
35	30738.0
36	34596.5
37	38455.0
38	43097.0
39	47739.0
40	47739.0
41	52654.5
42	57570.0
43	61767.0
44	65964.0
45	70378.5
46	74793.0
47	74793.0
48	78442.5
49	82092.0
50	82863.0
51	83634.0
52	81562.5
53	79491.0
54	79491.0
55	73973.0
56	68455.0
57	62617.0
58	56779.0
59	51003.5
60	45228.0
61	45228.0
62	38964.5
63	32701.0
64	27307.0
65	21913.0
66	17758.0
67	13603.0
68	13603.0
69	10971.5
70	8340.0
71	6781.5
72	5223.0
73	4223.5
74	3224.0
75	3224.0
76	2587.0
77	1950.0
78	1596.5
79	1243.0
80	1013.5
81	784.0
82	784.0
83	609.0
84	434.0
85	359.0
86	284.0
87	235.0
88	186.0
89	186.0
90	142.5
91	99.0
92	67.0
93	35.0
94	26.5
95	18.0
96	18.0
97	10.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	936950.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.79481529351382
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.43643756165841	62.921245069302635
2	7.793326989862795	11.346275974980362
3	2.4195747850102345	5.283974986909901
4	1.1115335899333654	3.2365552948694263
5	0.6710624518147245	2.442493361513269
6	0.40037356412755915	1.7487071789442883
7	0.2715123686353182	1.3835284907298695
8	0.17581239769072624	1.0238584812964968
9	0.1349956829063928	0.8844287222333359
>10	0.5667797163930951	6.542434399228878
>50	0.013322627849371736	0.6591116501731915
>100	0.004390220257818963	0.6243630334815452
>500	2.9268128676287797E-4	0.140510960983444
>1k	4.39021930144317E-4	0.9233881802915033
>5k	1.4634064338143898E-4	0.8391242150618564
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7877	0.8407065478414003	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3333	0.3557286941672448	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2981	0.3181599871924862	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2354	0.2512407278936976	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.201878435348738E-4
2	0.0	0.0	0.0	0.0	3.201878435348738E-4
3	0.0	0.0	0.0	0.0	3.201878435348738E-4
4	0.0	0.0	0.0	0.0	3.201878435348738E-4
5	0.0	0.0	0.0	0.0	3.201878435348738E-4
6	0.0	0.0	0.0	0.0	3.201878435348738E-4
7	0.0	0.0	0.0	0.0	3.201878435348738E-4
8	0.0	0.0	0.0	0.0	3.201878435348738E-4
9	0.0	0.0	0.0	0.0	5.336464058914563E-4
10	0.0	0.0	0.0	0.0	5.336464058914563E-4
11	1.0672928117829126E-4	0.0	0.0	0.0	5.336464058914563E-4
12	1.0672928117829126E-4	0.0	0.0	0.0	5.336464058914563E-4
13	1.0672928117829126E-4	0.0	0.0	0.0	5.336464058914563E-4
14	1.0672928117829126E-4	0.0	0.0	1.0672928117829126E-4	5.336464058914563E-4
15	1.0672928117829126E-4	0.0	0.0	3.201878435348738E-4	5.336464058914563E-4
16	1.0672928117829126E-4	0.0	0.0	6.403756870697476E-4	5.336464058914563E-4
17	1.0672928117829126E-4	0.0	0.0	9.605635306046213E-4	5.336464058914563E-4
18	1.0672928117829126E-4	0.0	0.0	0.0010672928117829127	5.336464058914563E-4
19	1.0672928117829126E-4	0.0	0.0	0.0010672928117829127	6.403756870697476E-4
20	1.0672928117829126E-4	0.0	0.0	0.002027856342387534	6.403756870697476E-4
21	1.0672928117829126E-4	0.0	0.0	0.002454773467100699	6.403756870697476E-4
22	1.0672928117829126E-4	0.0	0.0	0.00501627621537969	6.403756870697476E-4
23	1.0672928117829126E-4	0.0	0.0	0.0062970275895191845	7.471049682480389E-4
24	1.0672928117829126E-4	0.0	0.0	0.008751801056619884	7.471049682480389E-4
25	1.0672928117829126E-4	0.0	0.0	0.009925823149581087	7.471049682480389E-4
26	1.0672928117829126E-4	0.0	0.0	0.01109984524254229	7.471049682480389E-4
27	1.0672928117829126E-4	0.0	0.0	0.016649767863813437	7.471049682480389E-4
28	1.0672928117829126E-4	0.0	0.0	0.04365227600192113	7.471049682480389E-4
29	1.0672928117829126E-4	0.0	0.0	0.10107262927584183	7.471049682480389E-4
30	1.0672928117829126E-4	0.0	0.0	0.18336090506430439	7.471049682480389E-4
31	1.0672928117829126E-4	0.0	0.0	0.5216927263994877	7.471049682480389E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	635	0.0	19.228346	37
CCGACAT	70	1.2188923E-4	18.5	14
CGGTAGT	70	1.2188923E-4	18.5	1
GGTCTAC	155	2.0008883E-11	17.903225	1
GTTCTAA	85	2.7222462E-5	17.411764	1
TAGGAGT	130	1.3920726E-8	17.076923	4
CGACGTC	70	0.0025920144	15.857142	36
AACGCAG	3040	0.0	15.518092	5
TCTTATA	1085	0.0	15.5161295	37
GTACTTA	275	0.0	15.472728	30
TACTTAC	265	0.0	15.358491	31
AGTGTAC	320	0.0	15.03125	27
TTACCCT	100	1.0930213E-4	14.799999	4
GGAGTGT	365	0.0	14.69863	6
TTATGAC	140	5.990896E-7	14.535714	3
GTCTAAA	140	5.990896E-7	14.535714	1
TTAGGCT	80	0.0062974766	13.875	4
CGACCGA	80	0.0062974766	13.875	18
CGACAAA	80	0.0062974766	13.875	14
CATTACG	160	1.781791E-7	13.875	24
>>END_MODULE