FastQCFastQC Report
Thu 9 Feb 2017
SRR2727363.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727363.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2751646
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT201960.7339606911644885No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT74210.2696931218623326No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73320.2664586941779575No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT57180.2078028932500765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC21300.017.7183137
GTACTAA908.2791847E-414.3888881
TCTTATA36450.014.36351137
GTTCTAG3250.014.2307691
TATACAC8100.014.16049437
TATACTG4100.013.9878045
GTACTAG2102.044544E-913.2142851
TATACTA2102.044544E-913.2142855
TACTTAC4500.013.15555631
AGTGTAC6650.013.07518827
CGATAGA850.00940908413.05882525
GTACTTA4750.012.85263230
GTCTAGA2501.6007107E-1012.581
CTCTTAT55050.012.56857437
TCGTTAC2653.274181E-1112.56603823
GTCTAAG4150.012.4819281
TTTATAC4300.012.4767443
AACGCAG104650.012.4452945
GTATTAG2401.1295924E-912.3333341
GGACCGT1653.8083926E-612.3333346
Produced by FastQC (version 0.11.5)